Radiation protection of in vitro mammalian cells: effects of hydroxyl radical scavengers on the slopes and shoulders of survival curves

Journal Article · Wed May 01 00:00:00 EDT 1991 · Radiation Research; (USA)

DOI:https://doi.org/10.2307/3577817· OSTI ID:5543901

Ewing, D; Walton, H L ^[1]

---

1. Hahnemann Univ., Philadelphia, PA (USA)

We have tested several chemical compounds, characterized and widely used as hydroxyl radical (.OH) scavengers, for their effects on the radiation sensitivity of Chinese hamster V79 cells irradiated in air or nitrogen. Our purpose is to reexamine the proposed relationship between the level of protection and the rates at which the scavengers react with .OH. We found that the additives can have two apparently independent effects on the shape of survival curves: a reduction in sensitivity (i.e., 'protection,' a decrease in the value of k) and an increase in the size of the shoulder of the survival curve (an increase in the value of Dq). We measured intracellular scavenger concentrations, and, using these values in our analysis, we found that neither of the two effects is correlated with the rates at which the scavengers react with .OH. Although these results could mean that .OH do not cause lethal damage, the interpretation we believe most probably correct is that these scavengers protect in multiple ways. The protection would occur in addition to or instead of simple .OH removal.

• OSTI ID: 5543901
• Journal Information: Radiation Research; (USA), Vol. 126:2; ISSN 0033-7587
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
RADIATION PROTECTION
HYDROXYL RADICALS
RADIOSENSITIVITY EFFECTS
DOSE-RESPONSE RELATIONSHIPS
HAMSTERS
LUNGS
SURVIVAL CURVES
ANIMALS
BODY
MAMMALS
ORGANS
RADICALS
RESPIRATORY SYSTEM
RODENTS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture