Changes in the nuclear protein kinase activities in the regenerating liver of partially irradiated rat
- National Institute of Radiological Sciences, Tokyo (Japan)
X rays (4.8 Gy) inhibit both DNA synthesis and phosphorylation of histone H1 in the regenerating liver of the rat. To determine the cause of the inhibition of histone H1 phosphorylation, changes in the nuclear protein kinase activities during the prereplicative phase of regeneration were measured. The cAMP-dependent protein kinase activity was low during regeneration, and the changes in the activity were not statistically significant. The cAMP-independent protein kinase activity increased at 15 h, decreased at 18 h, and increased again at 24 h after partial hepatectomy. X irradiation prior to partial hepatectomy did not inhibit the increase at 15 h, but it did inhibit the increase at 24 h. The activity was not inhibited by isoquinolinesulfonamide inhibitors such as H-7, and it was activated by a commercial preparation of an inhibitor protein of the cAMP-dependent kinase. It was also inhibited by quercetin. The possibility that the radiation-sensitive nuclear protein kinase is a nuclear cAMP-independent protein kinase specific for histone H1 is considered.
- OSTI ID:
- 5466789
- Journal Information:
- Radiation Research; (USA), Vol. 119:3; ISSN 0033-7587
- Country of Publication:
- United States
- Language:
- English
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Synthesis and phosphorylation of histone H1 and high mobility group proteins in the regenerating rat liver after X irradiation
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HISTONES
PHOSPHORYLATION
LIVER
BIOLOGICAL REGENERATION
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
BIOLOGICAL RADIATION EFFECTS
CELL NUCLEI
DNA REPLICATION
HEPATECTOMY
INHIBITION
RATS
X RADIATION
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BODY
CELL CONSTITUENTS
CHEMICAL REACTIONS
DIGESTIVE SYSTEM
ELECTROMAGNETIC RADIATION
ENZYMES
GLANDS
IONIZING RADIATIONS
MAMMALS
MEDICINE
NUCLEIC ACID REPLICATION
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RECOVERY
RODENTS
SURGERY
TRANSFERASES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals