Antigen-specific T8/sup +/ human clone of cells with a nonspecific augmenting function on the T4 cell-B cell helper interaction
The authors isolated a T8/sup +/ T3/sup +/ Ia/sup +/ clone of cells from the peripheral blood mononuclear cells of a healthy subject. The clone was expanded and maintained with autologous feed cells, interleukin 2, and a streptococcal antigen. The T8/sup +/ clone of cells responded specifically to the streptococcal antigen, in the absence of accessory cells,and released a soluble factor. Both the cloned cells and the corresponding soluble factor expressed augmenting helper but not suppressor activity. The augmenting helper activity for B cell antibody synthesis was demonstrable only in the presence of autologous T 4 cells. Radioimmunoassay was used to measure antibodies. Although stimulation of the T8/sup +/ cloned cells was antigen-specific, the resulting soluble factor elicited nonspecific antibody synthesis in the presence of T4 and B cells. The T8/sup +/ cloned cell-derived factor was adsorbed by B cells but not by T4 cells. Preliminary studies suggest that the factor has the properties of a B cell growth factor. They suggest that the T8/sup +/ population consists of functionally heterogeneous cell subsets, some that have suppressor function and others that augment the T4/sup +/ helper-inducer activity in B cell antibody synthesis.
- Research Organization:
- Guy's Hospital, London, England
- OSTI ID:
- 5435604
- Journal Information:
- J. Immunol.; (United States), Vol. 139:10
- Country of Publication:
- United States
- Language:
- English
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