Psoralen plus near-ultraviolet light: a possible new method for measuring DNA repair synthesis
A new method is proposed to inhibit semiconservative DNA synthesis in cultured cells while DNA repair synthesis is being measured. The cells are treated with the DNA-crosslinking agent Trioxalen (4,5,8-trimethylpsoralen) plus near-ultraviolet light, and consequently 99.5% inhibition of replicative DNA synthesis is achieved. Additional DNA-damaging agents induce thymidine incorporation into the double-stranded regions of the DNA. The new method gave results very similar to those obtained with the benzoylated naphthoylated DEAE (BND) cellulose method using three human fibroblast strains, of which one had deficient capacity for DNA repair synthesis following treatment with gamma rays and methyl methanesulfonate. The advantages of the new method are simplicity and rapidity, as well as the high extent to which replicative DNA synthesis is inhibited.
- Research Organization:
- Radiobiology Department, Nuclear Research Center, Negev, Beer-Sheva, Israel
- OSTI ID:
- 5392054
- Journal Information:
- Radiat. Res.; (United States), Vol. 95:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
BIOLOGICAL REPAIR
PSORALEN
BIOLOGICAL EFFECTS
ULTRAVIOLET RADIATION
CELL CULTURES
FIBROBLASTS
MAN
METHYL METHANESULFONATE
THYMIDINE
ANIMAL CELLS
ANIMALS
ANTICOAGULANTS
AZINES
BIOLOGICAL RECOVERY
CONNECTIVE TISSUE CELLS
COUMARINS
DRUGS
ELECTROMAGNETIC RADIATION
ESTERS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
MAMMALS
MUTAGENS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
PYRIMIDINES
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
SOMATIC CELLS
SULFONIC ACID ESTERS
VERTEBRATES
560114* - Radiation Effects on Biochemicals- In Animals- (-1987)
560121 - Radiation Effects on Cells- External Source- (-1987)