Psoralen plus near-ultraviolet light: a possible new method for measuring DNA repair synthesis

Journal Article · Thu Sep 01 04:00:00 EDT 1983 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576099· OSTI ID:5578080

Heimer, Y M ^[1]; Kol, R; Shiloh, Y; Riklis, E

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1. Nuclear Research Center, Negev, Israel

A new method is proposed to inhibit semiconservative DNA synthesis in cultured cells while DNA repair synthesis is being measured. The cells are treated with the DNA-crosslinking agent Trioxalen (4,5,8-trimethylpsoralen) plus near-ultraviolet light, and consequently 99.5% inhibition of replicative DNA synthesis is achieved. Additional DNA-damaging agents induce thymidine incorporation into the double-stranded regions of the DNA. The new method gave results very similar to those obtained with the benzoylated naphthoylated DEAE (BND) cellulose method using three human fibroblast strains, of which one had deficient capacity for DNA repair synthesis following treatment with ..gamma.. rays and methyl methanesulfonate. The advantages of the new method are simplicity and rapidity, as well as the high extent to which replicative DNA synthesis is inhibited.

• OSTI ID: 5578080
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 95:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

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560121 -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTICOAGULANTS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CONNECTIVE TISSUE CELLS
COUMARINS
DNA
DNA REPLICATION
DRUGS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
GAMMA RADIATION
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
LABELLED COMPOUNDS
MAMMALS
MAN
NEAR ULTRAVIOLET RADIATION
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PRIMATES
PSORALEN
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SKIN
SOMATIC CELLS
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
VERTEBRATES