DNA repair in baboon alveolar macrophages: a system for assessing biohazardous materials

Journal Article · Tue Jun 01 04:00:00 EDT 1976 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/0013-9351(76)90098-0· OSTI ID:7234010

Meltz, M L

The repair of DNA in the alveolar macrophages of the baboon has been investigated after treatment of the cells in vitro with ultraviolet light and with the alkylating agent, methyl methanesulfonate. Repair replication has been observed to occur after ultraviolet-light irradiation of macrophages attached to plastic dishes. The two different techniques used for measuring repair replication were a standard density-gradient procedure which separates normal-density, repair-replicated, preexisting DNA from semiconservatively synthesized DNA containing sufficient (/sup 3/H)bromodeoxyuridine to cause a density shift; and an alternative procedure applicable to nonproliferating cell systems wherein residual semiconservative synthesis is inhibited by hydroxyurea, and the increased incorporation of the DNA precursor (/sup 3/H)thymidine due to repair replication is measured. The latter technique was applied to the investigation of the concentration dependence of repair replication both after and during the treatment of the cells with the alkylating agent, methyl methanesulfonate. The labeling after treatment measures the repair of damage still present at the end of the treatment interval, while the labeling during treatment allows for greater sensitivity and detection of all repair occurring during the treatment period.

• Research Organization: Southwest Foundation for Research and Education, San Antonio
• OSTI ID: 7234010
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 11:3; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANIMAL CELLS
ANTIMITOTIC DRUGS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CONNECTIVE TISSUE CELLS
DNA
DNA REPLICATION
DRUGS
ELECTROMAGNETIC RADIATION
IRRADIATION
MACROPHAGES
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHAGOCYTES
RADIATIONS
RECOVERY
REPAIR
SOMATIC CELLS
ULTRAVIOLET RADIATION