Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: Alternative exon usage predicts two different precursor proteins

Journal Article · Mon Feb 01 00:00:00 EST 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.2.571· OSTI ID:5113609

Rorsman, F; Bywater, M; Knott, T J; Scott, J; Betsholtz, C

The human platelet-derived growth factor (PDGF) A-chain locus was characterized by restriction endonuclease analysis, and the nucleotide sequence of its exons was determined. Seven exons were identified, spanning approximately 22 kilobase pairs of genomic DNA. Alternative exon usage, identified by cDNA cloning, occurs in a human glioblastoma cell line and may give rise to two types of A-chain precursors with different C termini. The exon-intron arrangement was similar to that of the PDGF B-chain/sis locus and seemed to divide the precursor proteins into functional domains. Southern blot analysis of genomic DNA showed that a single PDGF A-chain gene was present in the human genome.

• Research Organization: Dept. of Pathology, Univ. Hospital, S-751 85 Uppsala (SE)
• OSTI ID: 5113609
• Journal Information: Mol. Cell. Biol.; (United States), Vol. 8:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD PLATELETS
GROWTH FACTORS
STRUCTURE-ACTIVITY RELATIONSHIPS
CELL CULTURES
DNA SEQUENCING
DNA-CLONING
ENDONUCLEASES
GENETIC MAPPING
GLIOMAS
MAN
PRECURSOR
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CLONING
DISEASES
DNA-ASE
ENZYMES
ESTERASES
HYDROLASES
MAMMALS
MAPPING
MATERIALS
MITOGENS
NEOPLASMS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PRIMATES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry