Human protein tyrosine phosphatase 1C (PTPN6) gene structure: Alternate promoter usage and exon skipping general multiple transcripts

Journal Article · Mon May 01 04:00:00 EDT 1995 · Genomics

DOI:https://doi.org/10.1006/geno.1995.1020· OSTI ID:114899

Banville, D; Stocco, R; Shen, S ^[1]

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1. Biotechnology Research Institute, Quebec (Canada)

PTPN6, a protein tyrosine phosphatase with two Src homology-2 domains, is expressed predominantly in cells of the hematopoietic lineage. In addition, cDNAs encoding isoforms of the enzyme differing from the latter at their N-termini have been detected in normal and transformed epithelial cells. To understand further the regulation and expression of the PTPN6 gene, the authors have isolated and characterized overlapping human genomic {lambda} clones containing the entire locus. The gene, located on chromosome 12, consists of 17 exons spanning 17 kb of DNA. Three nonhematopoietic PTPN6 transcripts were identified in a variety of cell lines and were shown to be transcribed from a common promoter. Alternate splicing and exon skipping result in transcripts encoding the two SH2 domains and minor transcripts from which part of or the entire N-terminal SH2 domain coding sequences are missing. The hematopoietic form of PTPN6 transcript is initiated at a downstream promotor separated by 7 kb from the first. This promoter is active exclusively in cells of the hematopoietic lineage. Characterization of the 5` ends of the PTPN6 mRNAs by RT-PCR and analysis of the flanking genomic sequences identified putative initiation sites within the two promoters. 44 refs., 5 figs., 1 tab.

• OSTI ID: 114899
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 27; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA SEQUENCING
DNA-CLONING
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 12
PHOSPHATASES
POLYMERASE CHAIN REACTION