Structure and chromosomal localization of the human gene of the phosphotyrosyl phosphatase activator (PTPA) of protein phosphatase 2A

Journal Article · Thu Jul 20 04:00:00 EDT 1995 · Genomics

DOI:https://doi.org/10.1006/geno.1995.1140· OSTI ID:390930

Van Hoof, C; Cayla, X; Merlevede, W; Goris, J ^[1]

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1. Katholieke Universiteit Leuven, Parish (France); and others

The PTPA gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. PTPA, cloned from human genomic libraries, is encoded by one single-copy gene, composed of 10 exons and 9 introns with a total length of about 60 kb. The transcription start site was determined, and the 5{prime} flanking sequence was analyzed for its potential as a promotor. This region lacks a TATA sequence in the appropriate position relative to the transcription start, is very GC-rich, and contains upstream of the transcription start four Sp1 sites, a feature common to many TATA-less promotors. Based on the homology with DNA binding consensus sequences of transcription factors, we identified in this promotor region several putative DNA binding sites for transcription factors, such as NF-{kappa}B, Myb, Ets-1, Myc, and ATF. Transfection experiments with a construct containing the PTPA promotor region inserted 5{prime} of a luciferase reporter gene revealed that the 5{prime} flanking sequence of the PTPA gene indeed displayed promotor activity that seems to be cell-line dependent. By fluorescence in situ hybridization and G-banding, the PTPA gene was localized to the 9q34 region. The PTPA gene is positioned centromeric of c-abl in a region embracing several genes implicated in oncogenesis. 28 refs., 8 figs., 1 tab.

• OSTI ID: 390930
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 28; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACID SEQUENCE
BANDING TECHNIQUES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
FLUORESCENCE
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 9
OLIGONUCLEOTIDES
ONCOGENIC TRANSFORMATIONS
PHOSPHATASES
PHOSPHORYLATION
POLYMERASE CHAIN REACTION
RFLPS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRANSCRIPTION
TRANSCRIPTION FACTORS