Structure and transcription of the human c-mpl gene (MPL)
- Institut National de la Sante et de la Recherche Medicale, Creteil (France)
- Institut National de Transfusion Sanguine and INSERM, Paris (France)
The human c-mpl proto-oncogene encodes a member of the cytokine receptor superfamily, expressed mainly in CD 34-positive hematopoietic progenitors and in the megakaryocytic lineage. To investigate the elements required for this tissue-specific expression, the authors cloned the human c-mpl gene (MPL) as well as the 5[prime] end of the mouse gene. The human c-mpl gene contains 12 exons distributed over 17 kb of DNA. Each of the two [open quotes]cytokine receptor domains[close quotes] of Mpl is encoded by a set of four exons, the transmembrane domain by a single exon and the cytoplasmic domain by two exons. They also describe how three types of mRNA, encoding different proteins, are generated. The major species contains all 12 exons; mRNAs encoding a protein with a smaller cytoplasmic domain are produced by termination of the transcript within intron 10, and mrNAs encoding a putatitive soluble form of the c-Mpl protein lack exons 9 and 10. The promoter regions of the human and mouse genes were characterized. These promoters are GC-rich and contain putative binding sites for proteins of the Ets and GATA families. Finally, they show that a 700-bp fragment of the human c-mpl promoter is active in the HEL and K562 cell lines, which express erythroid and megakaryocytic markers, but is inactive in the nonhematopoietic HeLa cell line and the Jurkat T lymphoid cell line. 47 refs., 6 figs., 1 tab.
- OSTI ID:
- 7062275
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 20:1; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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