Cloning of the gene coding for human class 1 heparin-binding growth factor and its expression in fetal tissues

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.9.6.2387· OSTI ID:5516931

Wang, W P; Lehtoma, K; Varban, M L; Krishnan, I; Chiu, I M ^[1]

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1. Dept. of Internal Medicine and Comprehensive Cancer Center, The Ohio State Univ. Columbus, OH (US)

The authors have identified four overlapping genomic DNA clones coding for human class 1 heparin-binding growth factor (HBGF-1), also known as acidic fibroblast growth factor, by screening genomic DNA libraries with an HBGF-1 cDNA probe. The exon-intron structure of the HBGF-1 gene was determined by Southern hybridization and nucleotide sequence analysis. The complete amino acid sequence of human HBGF-1 was deduced from the nucleotide sequence of these genomic DNA clones. The predicted amino acid sequence is identical to the published amino acid sequence determined by protein sequencing. Southern blot analysis of human DNA suggested that there is a single-copy gene coding for HGBF-1. A 4.5-kilobase mRNA and two minor species (3.4 and 2.0 kilobases) homologous to the HBGF-1 gene were detected in cellular RNA isolated from human adult brain and kidney. The HBGF-1 mRNAs from brain and kidney had slightly different sizes. The mechanism for the synthesis of different sizes of mRNA was not determined. They also detected HBFG-1 transcript from glioblastoma cells, fetal brain, and kidney but not from placenta or fetal liver. Since HBGF-1 is an angiogenic factor, these data suggest that it may play a role in embryonic angiogenesis during fetal development.

• OSTI ID: 5516931
• Journal Information: Molecular and Cellular Biology; (USA), Journal Name: Molecular and Cellular Biology; (USA) Vol. 9:6; ISSN 0270-7306; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACID SEQUENCE
ANIMAL CELLS
ANTICOAGULANTS
BIOLOGICAL PATHWAYS
BODY
BRAIN
CARBOHYDRATES
CELL CULTURES
CENTRAL NERVOUS SYSTEM
CLONING
CONNECTIVE TISSUE CELLS
DNA
DNA HYBRIDIZATION
DNA-CLONING
DRUGS
FIBROBLASTS
GENE REGULATION
GENES
GROWTH FACTORS
HEMATOLOGIC AGENTS
HEPARIN
HUMAN POPULATIONS
HYBRIDIZATION
KIDNEYS
MESSENGER-RNA
MITOGENS
MOLECULAR STRUCTURE
MUCOPOLYSACCHARIDES
NERVOUS SYSTEM
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHENOTYPE
POLYSACCHARIDES
POPULATIONS
PROBES
PROTEINS
RNA
SACCHARIDES
SIZE
SOMATIC CELLS
TUMOR CELLS