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Molecular cloning and characterization of a cDNA encoding the cerebrovascular and the neuritic plaque amyloid peptides

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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Deposits of amyloid fibers are found in large numbers in the walls of blood vessels and in neuritic plaques in the brains of patients with Alzheimer disease and adults with Down syndrome. The authors used the amino acid sequence of the amyloid peptide to synthesize oligonucleotide probes specific for the gene encoding this peptide. When a human brain cDNA library was screened with this probe, a clone was found with a 1.7-kilobase insert that contains a long open reading frame coding for 412 amino acid residues including the 28 amino acids of the amyloid peptide. RNA gel blots revealed that a 3.3-kilobase mRNA species was present in the brains of individuals with Alzheimer disease, with Down syndrome, or with not apparent neurological disorders. Southern blots showed that homologous genes are present in the genomic DNA of humans, rabbits, sheep, hamsters, and mice, suggesting that this gene has been conserved through mammalian evolution. Localization of the corresponding genomic sequences on human chromosome 21 suggest a genetic relationship between Alzheimer disease and Down syndrome, and it may explain the early appearance of large numbers of neuritic plaques in adult Down syndrome patients.

Research Organization:
Institute for Basic Research, Staten Island, NY
OSTI ID:
5356740
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:12; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EVOLUTION
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHROMOSOMES
CLONING
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
DNA-CLONING
ELECTROPHORESIS
EVEN-ODD NUCLEI
FIBERS
HAMSTERS
HETEROCHROMOSOMES
HUMAN CHROMOSOME 21
ISOTOPES
LIGHT NUCLEI
MAMMALS
MENTAL DISORDERS
MESSENGER-RNA
MICE
MOLECULAR STRUCTURE
NERVOUS SYSTEM
NERVOUS SYSTEM DISEASES
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATIENTS
PEPTIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
RNA
RODENTS
SULFUR 35
SULFUR ISOTOPES
VERTEBRATES