Alternatively spliced exons encode the tissue-specific 5{prime} termini of leukocyte pp52 and stromal cell S37 mRNA isoforms

Journal Article · Tue Mar 05 00:00:00 EST 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0129· OSTI ID:459001

Thompson, A A; May, W; Denny, C T ^[1]

---

1. UCLA School of Medicine, Los Angeles, CA (United States); and others

The pp52 gene encodes an intracellular, F-actin-binding phosphoprotein (also designated LSP1 and WP34) postulated to function in cytoskeleton dynamics and cell motility. We previously reported that different mRNA isoforms are expressed from this gene in cells of the leukocyte lineage versus mesodermally derived cells. These tissue-specific mRNA isoforms are identical except for 5{prime}-untranslated regions and sequences coding for unique N-termini of 23 and 21 amino acids, respectively. As this is a single-copy gene, we predicted that these tissue-specific mRNA isoforms would be generated by alternative RNA splicing. We report that the unique 5{prime} sequences in these mRNA isoforms are encoded in two separate exons containing ATG initiation codes. These features confirm that the pp52 and S37 mRNA isoforms are generated by alternative RNA splicing and establish that they are independently translated. Other results presented here indicate that the differential expression of these exons in leukocytes versus mesodermally derived cells is regulated at the level of transcription by tissue-specific promoters. 30 refs., 2 figs., 1 tab.

• OSTI ID: 459001
• Journal Information: Genomics, Vol. 32, Issue 3; Other Information: PBD: 5 Mar 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
HEREDITARY DISEASES
GENETICS
RNA
SPLICING
PHOSPHOPROTEINS
TRANSCRIPTION
GENE MUTATIONS
GENE REGULATION
DNA SEQUENCING
TISSUE DISTRIBUTION
STRUCTURE-ACTIVITY RELATIONSHIPS
CODONS
FLUORESCENCE
DNA HYBRIDIZATION
POLYMERASE CHAIN REACTION
AMINO ACIDS
MESSENGER-RNA
LEUKOCYTES
DNA-CLONING