Alternatively spliced pp52 mRNA in nonlymphoid stromal cells

Journal Article · Fri Jan 01 04:00:00 EST 1993 · Journal of Immunology; (United States)

OSTI ID:7063984

Gimble, J M; Dorheim, M A; Youkhana, K; Hudson, J; Nead, M; Kincade, P W ^[1]; Gilly, M; Wood, Jr, W J; Hermanson, G G; Wall, R ^[2]; Kuehl, M ^[3]

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1. Oklahoma Medical Research Foundation, Oklahoma City (United States)
2. Univ. of California, Los Angeles (United States); United States; Molecular Biological Inst., Los Angeles, CA (United States)
3. National Cancer Inst., Bethesda, MD (United States)

It has been hypothesized that lymphoid form of the 52-kDa phosphoprotein protein may play a role in lymphocyte signal transduction. The authors show that alternatively spliced mRNA are expressed from this gene in nonlymphoid cell lines (myocytes, stromal cells, fibroblasts). These cell lines do not express the 1.6-kb lymphoid cell-specific transcript. Instead, mRNA of 2.0 and 2.8 kb are detected in varying abundance. A full-length 2.0-kb cDNA has been cloned and sequenced from the BMS2 stromal cell line by conventional screening and polymerase chain reaction-based methods. This cDNA clone, designated S37, has a single open reading frame encoding a 328 amino acid peptide. The nucleotide sequence of the S37 stromal cell cDNA is identical to that of the lymphocyte derived pp52 cDNA from the 3[prime] poly(A) tail to the codon encoding the amino acid at residue 24. This region of the S37 cDNA clone encodes a protein that is identical to that encoded by the lymphoic pp52 cDNA and includes a casein kinase II phosphorylation site. However, the two clones differ in their 5[prime] nucleotide sequence and their NH[sub 3] terminal amino acid sequence. This organization is consistent with alternative exon utilization. These results suggest that tissue-specific control mechanisms are used to generate different forms of lymphoic form of the 52-kDa phosphoprotein mRNA in lymphoid cells versus mesoderm-derived, nonlymphoid cell lineages. 26 refs., 5 figs.

• OSTI ID: 7063984
• Journal Information: Journal of Immunology; (United States), Journal Name: Journal of Immunology; (United States) Vol. 150:1; ISSN JOIMA3; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL CELLS
ANIMAL TISSUES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DNA SEQUENCING
GENE REGULATION
GENES
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROTEIN STRUCTURE
RNA
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TISSUES
TRANSCRIPTION