Structural organization of mouse peroxisome proliferator-activated receptor {gamma} (mPPAR{gamma}) gene: Alternative promoter use and different splicing yield two mPPAR{gamma} isoforms

Journal Article · Tue Aug 15 04:00:00 EDT 1995 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.92.17.7921· OSTI ID:98862

Zhu, Y; Qi, C; Rao, M S ^[1]

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1. Northwestern Univ. Medical School, Chicago, IL (United States); and others

To gain insight into the regulation of expression of peroxisome proliferator-activated receptor (PPAR) isoforms, we have determined the structural organization of the mouse PPAR {gamma} (mPPAR{gamma}) gene. This gene extends >105 kb and gives rise to two mRNAs (mPPAR{gamma}1 and mPPAR{gamma}2) that differ at their 5{prime} ends. The mPPAR{gamma}2 cDNA encodes an additional 30 amino acids N-terminal to the first ATG codon of mPPAR{gamma}1 and reveals a different 5{prime} untranslated sequence. We show that mPPAR{gamma}1 mRNA is encoded by eight exons, whereas the mPPAR{gamma}2 mRNA is encoded by seven exons. Most of the 5{prime} untranslated sequence of mPPAR{gamma}1 mRNA is encoded by two exons, whereas the 5{prime} untranslated sequence of mPPAR{gamma}1 mRNA is encoded by two exons, whereas the 5{prime} untranslated sequence and the extra 30 N-terminal amino acids of mPPAR{gamma}2 are encoded by one exon, which is located between the second and third exons coding for mPPAR{gamma}1. The last six exons of mPPAR{gamma} gene code for identical sequences in mPPAR{gamma}1 and mPPAR{gamma}2 isoforms. The mPPAR{gamma}1 and mPPAR{gamma}2 isoforms are transcribed from different promoters. The mPPAR{gamma} gene has been mapped to chromosome 6 E3-F1 by in situ hybridization using a biotin-labeled probe. These results establish that at least one of the PPAR genes yields more than one protein product, similar to that encountered with retinoid X receptor and retinoic acid receptor genes. The existence of multiple PPAR isoforms transcribed from different promoters could increase in the diversity of ligand and tissue-specific transcriptional responses. 22 refs., 5 figs.

• Sponsoring Organization: USDOE
• OSTI ID: 98862
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 17 Vol. 92; ISSN PNASA6; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
GENES
MICE
MOLECULAR STRUCTURE