Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer

Zhang, Dezhi; Hegab, Hisham E.; Lvov, Yuri; Dale Snow, L.; Palmer, James

doi:10.1186/s40064-016-1682-y

Title: Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer

Journal Article · Wed Jan 20 00:00:00 EST 2016 · SpringerPlus

DOI:https://doi.org/10.1186/s40064-016-1682-y· OSTI ID:1272638

Zhang, Dezhi; Hegab, Hisham E.; Lvov, Yuri; Dale Snow, L.; Palmer, James

Cellulase was immobilized onto silica gel surfaces pretreated with (3-aminopropyl) triethoxy-silane (3-APTES), and glutaraldehyde (GA) was used as a cross-linker. A carboxymethyl cellulose sodium salt (CMC) solution was used for activity experiments. Protein assay was performed to determine the mass immobilized and compare with free enzyme. Cellulase was successfully demonstrated to be immobilized on the modified silica gel surface, and no detectable amount of enzyme was stripped off during the hydrolysis of the CMC solution. The specific activity of the immobilized cellulase is 7 ± 2 % compared to the similar amount of free cellulase. Significant activity over multiple reuses was observed. The seventh batch achieved 82 % activity of the initial batch, and the fifteenth batch retained 31 %. Lastly, it was observed that the immobilized cellulase retained 48 % of its initial activity after 4 days, and 22 % even after 14 days.

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Research Organization:: Louisiana Tech Univ., Ruston, LA (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE)

Grant/Contract Number:: EE0003120

OSTI ID:: 1272638

Alternate ID(s):: OSTI ID: 1242890

Journal Information:: SpringerPlus, Journal Name: SpringerPlus Vol. 5 Journal Issue: 1; ISSN 2193-1801

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Switzerland

Language:: English

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Cited by: 67 works

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59 BASIC BIOLOGICAL SCIENCES
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Title: Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer

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