Immobilization of cellulolytic and hemicellulolytic enzymes on inorganic supports

Journal Article · Thu Feb 05 04:00:00 EST 1987 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260290214· OSTI ID:6564169

Shimizu, K; Ishihara, M

Commercial cellulase preparations from Trichoderma viride and Aspergillus niger were immobilized on porous silica glass and ceramics such as alumina and titania with titanium tetrachloride (TiCl/sub 4/) and on their silanized derivatives with glutaraldehyde (GLUT). The amounts of the immobilized enzymes were in the range 10-50 mg/g carrier (dry) depending on the kind of carrier and immobilization method. Their activities toward carboxymethyl cellulose (CMC), xylan, aryl-..beta..-glucoside, and aryl-..beta..-xyloside were 3-53% of those of the native enzymes. The optimum pH of the enzymes shifted to the acidic side in most cases, whereas the optimum temperatures were nearly the same as those of native ones. The activity of immobilized enzyme preparations toward CMC did not change significantly during continuous operation over a period of 60 days. Finally, xylan was hydrolyzed with the immobilized enzymes, and the sugars formed were investigated. 22 reference.

• Research Organization: Forestry and Forest Products Research Institute, Ibaraki, Japan
• OSTI ID: 6564169
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 29:2; ISSN BIBIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALUMINIUM COMPOUNDS
ALUMINIUM OXIDES
ASPERGILLUS
CARBOHYDRATES
CELLULASE
CELLULOSE
CHALCOGENIDES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
IMMOBILIZED ENZYMES
LYSIS
MECHANICAL STRUCTURES
MINERALS
O-GLYCOSYL HYDROLASES
OPTIMIZATION
ORGANIC COMPOUNDS
OXIDE MINERALS
OXIDES
OXYGEN COMPOUNDS
PH VALUE
PLANTS
POLYSACCHARIDES
QUANTITY RATIO
SACCHARIDES
SILICA
SILICON COMPOUNDS
SILICON OXIDES
SOLVOLYSIS
SUPPORTS
TITANIUM COMPOUNDS
TITANIUM OXIDES
TRANSITION ELEMENT COMPOUNDS
TRICHODERMA
TRICHODERMA VIRIDE