Purification and immobilization of Aspergillus niger. beta. -xylosidase

Journal Article · Tue Jan 01 04:00:00 EST 1980 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260220603· OSTI ID:6758804

Oguntimein, G B; Reilly, P J

..beta..-Xylosidase from a commercial Aspergillus niger preparation was purified by differential ammonium sulfate precipitation and either gel permeation or cation exchange chromatography, giving 16-fold purification in 32% yield for the first technique or 27-fold purification in 19% yield for the second. Enzyme prepared by this method was immobilized to 10 different carriers, but only when it was bound to alumina with TiCl/sub 4/ and to alkylamine porous silica with glutaraldehyde were substantial efficiencies and stabilities achieved.

• Research Organization: Iowa State Univ., Ames
• OSTI ID: 6758804
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 22; ISSN BIBIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALUMINIUM COMPOUNDS
ALUMINIUM OXIDES
ASPERGILLUS
CARRIER MOBILITY
CHALCOGENIDES
CHROMATOGRAPHY
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
ION EXCHANGE CHROMATOGRAPHY
MINERALS
MOBILITY
OXIDES
OXYGEN COMPOUNDS
PLANTS
PRECIPITATION
PURIFICATION
SEPARATION PROCESSES
SILICA
SILICON COMPOUNDS
SILICON OXIDES