Properties of soluble and immobilized Aspergillus niger. beta. -xylosidase

Journal Article · Tue Jan 01 04:00:00 EST 1980 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260220604· OSTI ID:7030255

Oguntimein, G B; Reilly, P J

Aspergillus niger ..beta..-xylosidase was characterized when in soluble form and when immobilized to alkylamine porous silica with glutaraldehyde and to alumina with titanium tetrachloride. Energies of activation averaged 13.4 kcal/mol for the soluble enzyme, 9.0 kcal/mol when immobilized to alumina, and 8.0 kcal/mol when bound to silica. The highest activity of all forms of ..beta..-xylosidase was found near pH 3. The soluble enzyme was highly stable at pH 4, where lowest rates of decay occurred, and temperatures of 65/sup 0/C and below.

• Research Organization: Iowa State Univ., Ames
• OSTI ID: 7030255
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Journal Issue: 6 Vol. 22; ISSN BIBIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ACTIVATION ENERGY
ALUMINIUM COMPOUNDS
ALUMINIUM OXIDES
ASPERGILLUS
BIOLOGICAL EFFECTS
CHALCOGENIDES
CHEMICAL PROPERTIES
ENERGY
ENZYME ACTIVITY
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
MINERALS
OXIDES
OXYGEN COMPOUNDS
PH VALUE
PLANTS
SILICA
SILICON COMPOUNDS
SILICON OXIDES
SOLUBILITY
TEMPERATURE DEPENDENCE