Final Technical Report: DOE-Biological Ocean Margins Program. Microbial Ecology of Denitrifying Bacteria in the Coastal Ocean.
The focus of our research was to provide a comprehensive study of the bacterioplankton populations off the coast of New Jersey near the Rutgers University marine field station using terminal restriction fragment polymorphism analysis (TRFLP) coupled to 16S rRNA genes for large data set studies. Our three revised objectives to this study became: (1) to describe bacterioplankton population dynamics in the Mid Atlantic Bight using TRFLP analysis of 16S rRNA genes. (2) to determine whether spatial and temporal factors are driving bacterioplankton community dynamics in the MAB using monthly samping along our transect line over a 2-year period. (3) to identify dominant members of a coastal bacterioplankton population by clonal library analysis of 16S rDNA genes and sequencing of PCR product corresponding to specific TRFLP peaks in the data set. Although open ocean time-series sites have been areas of microbial research for years, relatively little was known about the population dynamics of bacterioplankton communities in the coastal ocean on kilometer spatial and seasonal temporal scales. To gain a better understanding of microbial community variability, monthly samples of bacterial biomass were collected in 1995-1996 along a 34-km transect near the Long-Term Ecosystem Observatory (LEO-15) off the New Jersey coast. Surface andmore »
- Publication Date:
- OSTI Identifier:
- Report Number(s):
- Final Technical Report
- DOE Contract Number:
- Resource Type:
- Research Org:
- Lee Kerkhof/Rutgers University
- Sponsoring Org:
- USDOE; USDOE EM Office of Science and Technology (EM-50)
- Country of Publication:
- United States
- 09 BIOMASS FUELS; 54 ENVIRONMENTAL SCIENCES; BACTERIA; BIOMASS; COMMUNITIES; ECOLOGY; ECOSYSTEMS; GENES; MID-ATLANTIC BIGHT; NUTRIENTS; PHYTOPLANKTON; POPULATION DYNAMICS; SAMPLING; SIMULATION
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