The impact of simultaneous inoculation of Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans on rodent burn wounds
Abstract
Burn wound infection often involves a diverse combination of bacterial and fungal pathogens. In this study, we characterize the mixed species burn wound infection by inoculating the burn surface with 1 × 103/4/5 CFU of Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans in a 1:1:1 ratio. Using the revised Walker–Mason scald burn rat model, 168 male Sprague-Dawley rats (350–450 g) subject to ~10% TBSA burn injury, with or without inoculation, were evaluated for 11 days after burn. In the wound, P. aeruginosa and S. aureus formed robust biofilms as determined by the bacterial tissue load, ~1 × 109 CFU/g, and expression of key biofilm genes. Interestingly, within 3 days C. albicans achieved tissue loads of ~1 × 106 CFU/g, but its numbers were significantly reduced beyond the limit of detection in the burn wound by day 7 in partial-thickness injuries and by day 11 in full-thickness injuries. The pathogenic biofilms contributed to burn depth progression, increased release of HMGB-1 into circulation from injured tissue, and significantly elevated the numbers of circulating innate immune cells (Neutrophils, Monocytes, and Basophils). This robust model of multi-species burn wound infection will serve as the basis for the development of new antimicrobials for combating biofilm-basedmore »
- Authors:
-
[1];
[1];
[1];
[1]
- US Army Institute of Surgical Research, JBSA Fort Sam Houston, TX (United States)
- Publication Date:
- Research Org.:
- Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1981548
- Grant/Contract Number:
- SC0014664
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Burns
- Additional Journal Information:
- Journal Volume: 47; Journal Issue: 8; Journal ID: ISSN 0305-4179
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; Pseudomonas aeruginosa; Staphylococcus aureus; Candida albicans; biofilm; damage-associated molecular patterns; Walker–Mason rat burn model
Citation Formats
Brandenburg, Kenneth S., Weaver Jr., Alan J., Karna, S.L. Rajasekhar, and Leung, Kai P. The impact of simultaneous inoculation of Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans on rodent burn wounds. United States: N. p., 2021.
Web. doi:10.1016/j.burns.2021.02.025.
Brandenburg, Kenneth S., Weaver Jr., Alan J., Karna, S.L. Rajasekhar, & Leung, Kai P. The impact of simultaneous inoculation of Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans on rodent burn wounds. United States. https://doi.org/10.1016/j.burns.2021.02.025
Brandenburg, Kenneth S., Weaver Jr., Alan J., Karna, S.L. Rajasekhar, and Leung, Kai P. Thu .
"The impact of simultaneous inoculation of Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans on rodent burn wounds". United States. https://doi.org/10.1016/j.burns.2021.02.025. https://www.osti.gov/servlets/purl/1981548.
@article{osti_1981548,
title = {The impact of simultaneous inoculation of Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans on rodent burn wounds},
author = {Brandenburg, Kenneth S. and Weaver Jr., Alan J. and Karna, S.L. Rajasekhar and Leung, Kai P.},
abstractNote = {Burn wound infection often involves a diverse combination of bacterial and fungal pathogens. In this study, we characterize the mixed species burn wound infection by inoculating the burn surface with 1 × 103/4/5 CFU of Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans in a 1:1:1 ratio. Using the revised Walker–Mason scald burn rat model, 168 male Sprague-Dawley rats (350–450 g) subject to ~10% TBSA burn injury, with or without inoculation, were evaluated for 11 days after burn. In the wound, P. aeruginosa and S. aureus formed robust biofilms as determined by the bacterial tissue load, ~1 × 109 CFU/g, and expression of key biofilm genes. Interestingly, within 3 days C. albicans achieved tissue loads of ~1 × 106 CFU/g, but its numbers were significantly reduced beyond the limit of detection in the burn wound by day 7 in partial-thickness injuries and by day 11 in full-thickness injuries. The pathogenic biofilms contributed to burn depth progression, increased release of HMGB-1 into circulation from injured tissue, and significantly elevated the numbers of circulating innate immune cells (Neutrophils, Monocytes, and Basophils). This robust model of multi-species burn wound infection will serve as the basis for the development of new antimicrobials for combating biofilm-based wound infections.},
doi = {10.1016/j.burns.2021.02.025},
journal = {Burns},
number = 8,
volume = 47,
place = {United States},
year = {Thu Mar 04 00:00:00 EST 2021},
month = {Thu Mar 04 00:00:00 EST 2021}
}
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