Defense of the lungs in oxygen-injured baboons
Pneumonia and subsequent sepsis have been linked with multiple organ failure and death in patients with adult respiratory distress syndrome. This suggests that lung defense may be impaired. In a model of ARDS, 13 baboons were intubated and ventilated with 80% O/sub 2/ for 6 days, following which 7 were inoculated intratracheally with Pseudomonas aeruginosa (PA) strain DGI-RI30. After 5 days on 50% O/sub 2/ the animals were sacrificed. Bronchoalveolar lavage (BAL) was performed before O/sub 2/ exposure, after 6 days of 80% O/sub 2/, and at sacrifice. Total WBC and differential counts were determined for BAL cells. Mononuclear BAL cells were isolated for in vitro phagocytosis assays. BAL fluid (BALF) was tested for in vitro PMN chemotactic activity. An index of bacterial infection (sum of log CFU/g lung homogenate) was greater for infected animals (p < 0.01). Percentage of PMN in BALF was significantly increased at sacrifice in PA-inoculated animals. BALF from PA-inoculated animals at sacrifice elicited increased in vitro PMN chemotaxis, which was destroyed at 100/sup 0/C but not 56/sup 0/C, extracted by ether, and not degraded by proteases. Mononuclear BAL cells from inoculated animals at sacrifice were phagocytially more active than baseline cells. These data suggest that oxygen injury does not inhibit recruitment of PMN or phagocytosis of bacteria by alveolar macrophages in baboons; however, the addition of bacterial inoculation results in clinical deterioration despite unimpaired local defense.
- Research Organization:
- Univ. of Texas Health Science Center, San Antonio
- OSTI ID:
- 7021955
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Suppression in lung defense responses after bacterial infection in rats pretreated with different welding fumes
A comparison of the pulmonary defenses against streptococcal infection in rats and mice following O3 exposure: Differences in disease susceptibility and neutrophil recruitment
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
APES
BACTERIAL DISEASES
LUNGS
INJURIES
OXYGEN
TOXICITY
BIOLOGICAL MODELS
INFECTIVITY
LAVAGE
MACROPHAGES
NEUTROPHILS
PHAGOCYTOSIS
PSEUDOMONAS
ANIMAL CELLS
ANIMALS
BACTERIA
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
ELEMENTS
INFECTIOUS DISEASES
LEUKOCYTES
MAMMALS
MATERIALS
MICROORGANISMS
NONMETALS
ORGANS
PHAGOCYTES
PRIMATES
RESPIRATORY SYSTEM
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550900 - Pathology