Plant biomass fermentation by the extreme thermophile Caldicellulosiruptor bescii for co-production of green hydrogen and acetone: Technoeconomic analysis
Abstract
A variety of chemical and biological processes have been proposed for conversion of sustainable low-cost feedstocks into industrial products. Here, a biorefinery concept is formulated, modeled, and analyzed in which a naturally (hemi)cellulolytic and extremely thermophilic bacterium, Caldicellulosiruptor bescii, is metabolically engineered to convert the carbohydrate content of lignocellulosic biomasses (i.e., soybean hulls, transgenic poplar) into green hydrogen and acetone. Experimental validation of C. bescii fermentative performance demonstrated 82% carbohydrate solubilization of soybean hulls and 55% for transgenic poplar. A detailed technical design, including equipment specifications, provides the basis for an economic analysis that establishes metabolic engineering targets. This robust industrial process leveraging metabolically engineered C. bescii yields 206 kg acetone and 25 kg H2 per metric ton of soybean hull, or 174 kg acetone and 21 kg H2 per metric ton transgenic poplar. Beyond this specific case, the model demonstrates industrial feasibility and economic advantages of thermophilic fermentation.
- Authors:
-
- North Carolina State University, Raleigh, NC (United States)
- University of Georgia, Athens, GA (United States)
- Publication Date:
- Research Org.:
- Univ. of Georgia, Athens, GA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); US Department of Agriculture (USDA); National Science Foundation (NSF); US DoEd GAANN Fellowship; National Institutes of Health (NIH)
- OSTI Identifier:
- 1976891
- Grant/Contract Number:
- SC0019391; SC0022192; 2018-67021-27716; CBET-1802939; P200A160061; NIH T32 GM008776-16
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Bioresource Technology
- Additional Journal Information:
- Journal Volume: 348; Journal Issue: C; Journal ID: ISSN 0960-8524
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION; 59 BASIC BIOLOGICAL SCIENCES; acetone; green hydrogen; Caldicellulosiruptor bescii; extreme thermophiles; technoeconomic analysis
Citation Formats
Bing, Ryan G., Straub, Christopher T., Sulis, Daniel B., Wang, Jack P., Adams, Michael W.W., and Kelly, Robert M. Plant biomass fermentation by the extreme thermophile Caldicellulosiruptor bescii for co-production of green hydrogen and acetone: Technoeconomic analysis. United States: N. p., 2022.
Web. doi:10.1016/j.biortech.2022.126780.
Bing, Ryan G., Straub, Christopher T., Sulis, Daniel B., Wang, Jack P., Adams, Michael W.W., & Kelly, Robert M. Plant biomass fermentation by the extreme thermophile Caldicellulosiruptor bescii for co-production of green hydrogen and acetone: Technoeconomic analysis. United States. https://doi.org/10.1016/j.biortech.2022.126780
Bing, Ryan G., Straub, Christopher T., Sulis, Daniel B., Wang, Jack P., Adams, Michael W.W., and Kelly, Robert M. Sat .
"Plant biomass fermentation by the extreme thermophile Caldicellulosiruptor bescii for co-production of green hydrogen and acetone: Technoeconomic analysis". United States. https://doi.org/10.1016/j.biortech.2022.126780. https://www.osti.gov/servlets/purl/1976891.
@article{osti_1976891,
title = {Plant biomass fermentation by the extreme thermophile Caldicellulosiruptor bescii for co-production of green hydrogen and acetone: Technoeconomic analysis},
author = {Bing, Ryan G. and Straub, Christopher T. and Sulis, Daniel B. and Wang, Jack P. and Adams, Michael W.W. and Kelly, Robert M.},
abstractNote = {A variety of chemical and biological processes have been proposed for conversion of sustainable low-cost feedstocks into industrial products. Here, a biorefinery concept is formulated, modeled, and analyzed in which a naturally (hemi)cellulolytic and extremely thermophilic bacterium, Caldicellulosiruptor bescii, is metabolically engineered to convert the carbohydrate content of lignocellulosic biomasses (i.e., soybean hulls, transgenic poplar) into green hydrogen and acetone. Experimental validation of C. bescii fermentative performance demonstrated 82% carbohydrate solubilization of soybean hulls and 55% for transgenic poplar. A detailed technical design, including equipment specifications, provides the basis for an economic analysis that establishes metabolic engineering targets. This robust industrial process leveraging metabolically engineered C. bescii yields 206 kg acetone and 25 kg H2 per metric ton of soybean hull, or 174 kg acetone and 21 kg H2 per metric ton transgenic poplar. Beyond this specific case, the model demonstrates industrial feasibility and economic advantages of thermophilic fermentation.},
doi = {10.1016/j.biortech.2022.126780},
journal = {Bioresource Technology},
number = C,
volume = 348,
place = {United States},
year = {Sat Jan 29 00:00:00 EST 2022},
month = {Sat Jan 29 00:00:00 EST 2022}
}
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