Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii
Abstract
Microbial fermentation of lignocellulosic biomass to produce industrial chemicals is exacerbated by the recalcitrant network of lignin, cellulose and hemicelluloses comprising the plant secondary cell wall. In this study, we show that transgenic poplar (Populus trichocarpa) lines can be solubilized without any pretreatment by the extreme thermophile Caldicellulosiruptor bescii that has been metabolically engineered to shift its fermentation products away from inhibitory organic acids to ethanol. Carbohydrate solubilization and conversion of unpretreated milled biomass is nearly 90% for two transgenic lines, compared to only 25% for wild-type poplar. Unexpectedly, unpretreated intact poplar stems achieved nearly 70% of the fermentation production observed with milled poplar as the substrate. The nearly quantitative microbial conversion of the carbohydrate content of unpretreated transgenic lignocellulosic biomass bodes well for full utilization of renewable biomass feedstocks.
- Authors:
-
[1];
[1];
[2];
[1];
[1]
- North Carolina State Univ., Raleigh, NC (United States)
- Univ. of Georgia, Athens, GA (United States)
- Publication Date:
- Research Org.:
- Univ. of Georgia, Athens, GA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1613208
- Grant/Contract Number:
- SC0019391
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; Science & Technology
Citation Formats
Straub, Christopher T., Khatibi, Piyum A., Wang, Jack P., Conway, Jonathan M., Williams-Rhaesa, Amanda M., Peszlen, Ilona M., Chiang, Vincent L., Adams, Michael W. W., and Kelly, Robert M. Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii. United States: N. p., 2019.
Web. doi:10.1038/s41467-019-11376-6.
Straub, Christopher T., Khatibi, Piyum A., Wang, Jack P., Conway, Jonathan M., Williams-Rhaesa, Amanda M., Peszlen, Ilona M., Chiang, Vincent L., Adams, Michael W. W., & Kelly, Robert M. Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii. United States. https://doi.org/10.1038/s41467-019-11376-6
Straub, Christopher T., Khatibi, Piyum A., Wang, Jack P., Conway, Jonathan M., Williams-Rhaesa, Amanda M., Peszlen, Ilona M., Chiang, Vincent L., Adams, Michael W. W., and Kelly, Robert M. Wed .
"Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii". United States. https://doi.org/10.1038/s41467-019-11376-6. https://www.osti.gov/servlets/purl/1613208.
@article{osti_1613208,
title = {Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii},
author = {Straub, Christopher T. and Khatibi, Piyum A. and Wang, Jack P. and Conway, Jonathan M. and Williams-Rhaesa, Amanda M. and Peszlen, Ilona M. and Chiang, Vincent L. and Adams, Michael W. W. and Kelly, Robert M.},
abstractNote = {Microbial fermentation of lignocellulosic biomass to produce industrial chemicals is exacerbated by the recalcitrant network of lignin, cellulose and hemicelluloses comprising the plant secondary cell wall. In this study, we show that transgenic poplar (Populus trichocarpa) lines can be solubilized without any pretreatment by the extreme thermophile Caldicellulosiruptor bescii that has been metabolically engineered to shift its fermentation products away from inhibitory organic acids to ethanol. Carbohydrate solubilization and conversion of unpretreated milled biomass is nearly 90% for two transgenic lines, compared to only 25% for wild-type poplar. Unexpectedly, unpretreated intact poplar stems achieved nearly 70% of the fermentation production observed with milled poplar as the substrate. The nearly quantitative microbial conversion of the carbohydrate content of unpretreated transgenic lignocellulosic biomass bodes well for full utilization of renewable biomass feedstocks.},
doi = {10.1038/s41467-019-11376-6},
journal = {Nature Communications},
number = 1,
volume = 10,
place = {United States},
year = {Wed Aug 07 00:00:00 EDT 2019},
month = {Wed Aug 07 00:00:00 EDT 2019}
}
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Works referenced in this record:
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Complete Proteomic-Based Enzyme Reaction and Inhibition Kinetics Reveal How Monolignol Biosynthetic Enzyme Families Affect Metabolic Flux and Lignin in Populus trichocarpa
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journal, July 2010
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Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis
journal, April 2018
- Wang, Jack P.; Matthews, Megan L.; Williams, Cranos M.
- Nature Communications, Vol. 9, Issue 1
Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass
journal, February 2011
- Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808
Direct conversion of plant biomass to ethanol by engineered Caldicellulosiruptor bescii
journal, June 2014
- Chung, D.; Cha, M.; Guss, A. M.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 24, p. 8931-8936
Complete Proteomic-Based Enzyme Reaction and Inhibition Kinetics Reveal How Monolignol Biosynthetic Enzyme Families Affect Metabolic Flux and Lignin in Populus trichocarpa
journal, March 2014
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Thermophilic lignocellulose deconstruction
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A Highly Thermostable Kanamycin Resistance Marker Expands the Tool Kit for Genetic Manipulation of Caldicellulosiruptor bescii
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- Biotechnology for Biofuels, Vol. 12, Issue 1
Works referencing / citing this record:
Use of the lignocellulose-degrading bacterium Caldicellulosiruptor bescii to assess recalcitrance and conversion of wild-type and transgenic poplar
journal, March 2020
- Straub, Christopher T.; Bing, Ryan G.; Wang, Jack P.
- Biotechnology for Biofuels, Vol. 13, Issue 1