The bamboo delignification saturation point in alkaline hydrogen peroxide pretreatment and its association with enzymatic hydrolysis

Huang, Chen; Zhan, Yunni; Cheng, Jinyuan; Wang, Jia; Meng, Xianzhi; Fang, Guigan; Ragauskas, Arthur J.

doi:10.1016/j.biortech.2022.127462

Title: The bamboo delignification saturation point in alkaline hydrogen peroxide pretreatment and its association with enzymatic hydrolysis

Abstract

A delignification saturation point (DSP) was observed for bamboo alkaline hydrogen peroxide pretreatment (AHP). Lignin removal was increased from 52.23% to ~70% when increasing H₂O₂ dosage from 0% to 2% at the optimum pH, but it cannot be further reinforced as increasing the H₂O₂. With partial lignin preserved, the glucan hydrolysis yield was found to have a ceiling of ~80%. This work indicated a strong association between enzymatic digestibility and lignin removal. Anatomical analysis by fluorescence microscope and confocal Raman microscope revealed that the undegradable lignin was mainly existing in the cell corner of sclerenchyma fibers, causing the DSP in the bamboo AHP. Finally, the residual lignin in pretreated bamboo was characterized with GPC, HSQC NMR, and ³¹P NMR, which revealed the nature of DSP. This study could help to understand the lignin modification during the AHP and further contribute to the establishment of a chemical-saving biorefinery.

Authors:

Huang, Chen ^[1]; Zhan, Yunni ^[1]; Cheng, Jinyuan ^[1]; Wang, Jia ^[2]; Meng, Xianzhi ^[3]; Fang, Guigan ^[1];

^[3]

Chinese Academy of Forestry, Nanjing (China)
Nanjing Forestry Univ. (China)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Publication Date:: Sat Jun 11 00:00:00 EDT 2022

Research Org.:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE; National Natural Science Foundation for Youth; Jiangsu Province Key Laboratory of Biomass Engineering and Materials; Natural Science Foundation of Jiangsu Province; National Natural Science Foundation of China (NSFC); University of Tennessee

OSTI Identifier:: 1883823

Grant/Contract Number:: AC05-00OR22725; 32001273; JSBEM-S-202004; JSBEM-S-202203; BK2020789; 52006106

Resource Type:: Accepted Manuscript

Journal Name:: Bioresource Technology

Additional Journal Information:: Journal Volume: 359; Journal Issue: 1; Journal ID: ISSN 0960-8524

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 09 BIOMASS FUELS; bamboo; alkaline hydrogen peroxide pretreatment; delignification saturation point; enzymatic saccharification

Citation Formats


                    Huang, Chen, Zhan, Yunni, Cheng, Jinyuan, Wang, Jia, Meng, Xianzhi, Fang, Guigan, and Ragauskas, Arthur J. The bamboo delignification saturation point in alkaline hydrogen peroxide pretreatment and its association with enzymatic hydrolysis.  United States: N. p., 2022. 
Web.  doi:10.1016/j.biortech.2022.127462.

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                    Huang, Chen, Zhan, Yunni, Cheng, Jinyuan, Wang, Jia, Meng, Xianzhi, Fang, Guigan, & Ragauskas, Arthur J. The bamboo delignification saturation point in alkaline hydrogen peroxide pretreatment and its association with enzymatic hydrolysis.  United States.  https://doi.org/10.1016/j.biortech.2022.127462

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                    Huang, Chen, Zhan, Yunni, Cheng, Jinyuan, Wang, Jia, Meng, Xianzhi, Fang, Guigan, and Ragauskas, Arthur J. Sat .  
"The bamboo delignification saturation point in alkaline hydrogen peroxide pretreatment and its association with enzymatic hydrolysis".  United States.  https://doi.org/10.1016/j.biortech.2022.127462.  https://www.osti.gov/servlets/purl/1883823.

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@article{osti_1883823,

  title        = {The bamboo delignification saturation point in alkaline hydrogen peroxide pretreatment and its association with enzymatic hydrolysis},

  author       = {Huang, Chen and Zhan, Yunni and Cheng, Jinyuan and Wang, Jia and Meng, Xianzhi and Fang, Guigan and Ragauskas, Arthur J.},

  abstractNote = {A delignification saturation point (DSP) was observed for bamboo alkaline hydrogen peroxide pretreatment (AHP). Lignin removal was increased from 52.23% to ~70% when increasing H2O2 dosage from 0% to 2% at the optimum pH, but it cannot be further reinforced as increasing the H2O2. With partial lignin preserved, the glucan hydrolysis yield was found to have a ceiling of ~80%. This work indicated a strong association between enzymatic digestibility and lignin removal. Anatomical analysis by fluorescence microscope and confocal Raman microscope revealed that the undegradable lignin was mainly existing in the cell corner of sclerenchyma fibers, causing the DSP in the bamboo AHP. Finally, the residual lignin in pretreated bamboo was characterized with GPC, HSQC NMR, and 31P NMR, which revealed the nature of DSP. This study could help to understand the lignin modification during the AHP and further contribute to the establishment of a chemical-saving biorefinery.},

  doi          = {10.1016/j.biortech.2022.127462},

  journal      = {Bioresource Technology},

  number       = 1,

  volume       = 359,

  place        = {United States},

  year         = {Sat Jun 11 00:00:00 EDT 2022},

  month        = {Sat Jun 11 00:00:00 EDT 2022}

}

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Title: The bamboo delignification saturation point in alkaline hydrogen peroxide pretreatment and its association with enzymatic hydrolysis

Abstract

Citation Formats

The characterization of pretreated lignocellulosic substrates prior to enzymatic hydrolysis, part 1: A modified Simons' staining technique journal, September 2008

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Facilitating enzymatic digestibility of larch by in-situ lignin modification during combined acid and alkali pretreatment journal, September 2020

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New strategy to elucidate the positive effects of extractable lignin on enzymatic hydrolysis by quartz crystal microbalance with dissipation journal, March 2019

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Increasing the Carbohydrate Output of Bamboo Using a Combinatorial Pretreatment journal, May 2020

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Features correlated to improved enzymatic digestibility of corn stover subjected to alkaline hydrogen peroxide pretreatment journal, April 2021

Identification of features associated with plant cell wall recalcitrance to pretreatment by alkaline hydrogen peroxide in diverse bioenergy feedstocks using glycome profiling journal, January 2014

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The characterization of pretreated lignocellulosic substrates prior to enzymatic hydrolysis, part 1: A modified Simons' staining technique
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A novel pre-treatment for the methane production from microalgae by using N-methylmorpholine-N-oxide (NMMO)
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Investigation of alkaline hydrogen peroxide pretreatment and Tween 80 to enhance enzymatic hydrolysis of sugarcane bagasse
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New strategy to elucidate the positive effects of extractable lignin on enzymatic hydrolysis by quartz crystal microbalance with dissipation
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Visualization of Miscanthus × giganteus cell wall deconstruction subjected to dilute acid pretreatment for enhanced enzymatic digestibility
journal, July 2015

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journal, January 2016

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The Nature of Hololignin
journal, November 2017

FTIR and XPS analysis of the changes in bamboo chemical structure decayed by white-rot and brown-rot fungi
journal, September 2013

The Path Forward for Biofuels and Biomaterials
journal, January 2006

Alkaline hydrogen peroxide pretreatment of bamboo culm for improved enzymatic release of reducing sugars using recombinant cellulases
journal, November 2019

Physical and chemical characterizations of corn stover and poplar solids resulting from leading pretreatment technologies
journal, September 2009

Significance of Lignin S/G Ratio in Biomass Recalcitrance of Populus trichocarpa Variants for Bioethanol Production
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Increasing the Carbohydrate Output of Bamboo Using a Combinatorial Pretreatment
journal, May 2020

Availability of four energy crops assessing by the enzymatic hydrolysis and structural features of lignin before and after hydrothermal treatment
journal, January 2018

Structural elucidation of inhomogeneous lignins from bamboo
journal, June 2015

Synergistic effect of hydrogen peroxide and ammonia on lignin
journal, April 2020

Investigating plant cell wall components that affect biomass recalcitrance in poplar and switchgrass
journal, January 2013

Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis
journal, May 2019

Evolution of the Lignin Chemical Structure during the Bioethanol Production Process and Its Inhibition to Enzymatic Hydrolysis
journal, April 2020

Insights into the effect of dilute acid, hot water or alkaline pretreatment on the cellulose accessible surface area and the overall porosity of Populus
journal, January 2015

Features correlated to improved enzymatic digestibility of corn stover subjected to alkaline hydrogen peroxide pretreatment
journal, April 2021

Identification of features associated with plant cell wall recalcitrance to pretreatment by alkaline hydrogen peroxide in diverse bioenergy feedstocks using glycome profiling
journal, January 2014

Lignin repolymerisation in spruce autohydrolysis pretreatment increases cellulase deactivation
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Modified alkaline peroxide pretreatment: An efficient path forward for bioethanol production from bamboo
journal, November 2020