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Title: Cosolvent enhanced lignocellulosic fractionation tailoring lignin chemistry and enhancing lignin bioconversion

Abstract

Cosolvent Enhanced Lignocellulosic Fractionation (CELF) is an emerging solvolysis pretreatment to fractionate lignocellulosic biomass. Herein, the bioconversion performance of CELF lignin was fully evaluated for the first time. Results showed that CELF lignin possessed higher content of carboxylic acid OH, lower molecular weight, and disappeared β-O-4 and β-5 linkages compared to other two technical lignins including a conventional ethanol organosolv lignin (EOL) and a kraft lignin (KL). Rhodococcus opacus PD630 cell count from CELF lignin fermentation reached the highest value of 3.9 107 CFU/mL, representing a 62.5% and 77.3% improvement over EOL and KL, respectively. Correspondingly, lipid yield reached 143 mg/L from CELF lignin, which was 36.2% and 26.5% higher than from EOL and KL, respectively. Principal component analysis (PCA) revealed that more carboxylic acid groups and lower molecular weight contributed to the enhanced bioconversion performance of CELF lignin. This study demonstrates that CELF lignin is a promising candidate for bioconversion.

Authors:
 [1];  [2];  [3]; ORCiD logo [4];  [5];  [5];  [3];  [3]; ORCiD logo [6]
+ Show Author Affiliations
  1. Inner Mongolia Univ., Hohhot (China); Univ. of Tennessee, Knoxville, TN (United States)
  2. Univ. of Tennessee, Knoxville, TN (United States)
  3. Univ. of California, Riverside, CA (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  5. Tianjin Univ., Tianjin (China)
  6. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Natural Science Foundation of China (NSFC)
OSTI Identifier:
1843678
Alternate Identifier(s):
OSTI ID: 1868602
Grant/Contract Number:  
AC05-00OR22725; 21706136; 21766025
Resource Type:
Accepted Manuscript
Journal Name:
Bioresource Technology
Additional Journal Information:
Journal Volume: 347; Journal Issue: TBD; Journal ID: ISSN 0960-8524
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; bioconversion; Co-solvent Enhanced Lignocellulosic Fractionation (CELF); Rhodococcus opacus PD630; Lignin valorization

Citation Formats

Zhao, Zhi-Min, Meng, Xianzhi, Scheidemantle, Brent, Pu, Yunqiao, Liu, Zhi-Hua, Li, Bing-Zhi, Wyman, Charles E., Cai, Charles M., and Ragauskas, Arthur J. Cosolvent enhanced lignocellulosic fractionation tailoring lignin chemistry and enhancing lignin bioconversion. United States: N. p., 2021. Web. doi:10.1016/j.biortech.2021.126367.
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Zhao, Zhi-Min, Meng, Xianzhi, Scheidemantle, Brent, Pu, Yunqiao, Liu, Zhi-Hua, Li, Bing-Zhi, Wyman, Charles E., Cai, Charles M., & Ragauskas, Arthur J. Cosolvent enhanced lignocellulosic fractionation tailoring lignin chemistry and enhancing lignin bioconversion. United States. https://doi.org/10.1016/j.biortech.2021.126367
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Zhao, Zhi-Min, Meng, Xianzhi, Scheidemantle, Brent, Pu, Yunqiao, Liu, Zhi-Hua, Li, Bing-Zhi, Wyman, Charles E., Cai, Charles M., and Ragauskas, Arthur J. Thu . "Cosolvent enhanced lignocellulosic fractionation tailoring lignin chemistry and enhancing lignin bioconversion". United States. https://doi.org/10.1016/j.biortech.2021.126367. https://www.osti.gov/servlets/purl/1843678.
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@article{osti_1843678,
title = {Cosolvent enhanced lignocellulosic fractionation tailoring lignin chemistry and enhancing lignin bioconversion},
author = {Zhao, Zhi-Min and Meng, Xianzhi and Scheidemantle, Brent and Pu, Yunqiao and Liu, Zhi-Hua and Li, Bing-Zhi and Wyman, Charles E. and Cai, Charles M. and Ragauskas, Arthur J.},
abstractNote = {Cosolvent Enhanced Lignocellulosic Fractionation (CELF) is an emerging solvolysis pretreatment to fractionate lignocellulosic biomass. Herein, the bioconversion performance of CELF lignin was fully evaluated for the first time. Results showed that CELF lignin possessed higher content of carboxylic acid OH, lower molecular weight, and disappeared β-O-4 and β-5 linkages compared to other two technical lignins including a conventional ethanol organosolv lignin (EOL) and a kraft lignin (KL). Rhodococcus opacus PD630 cell count from CELF lignin fermentation reached the highest value of 3.9 107 CFU/mL, representing a 62.5% and 77.3% improvement over EOL and KL, respectively. Correspondingly, lipid yield reached 143 mg/L from CELF lignin, which was 36.2% and 26.5% higher than from EOL and KL, respectively. Principal component analysis (PCA) revealed that more carboxylic acid groups and lower molecular weight contributed to the enhanced bioconversion performance of CELF lignin. This study demonstrates that CELF lignin is a promising candidate for bioconversion.},
doi = {10.1016/j.biortech.2021.126367},
journal = {Bioresource Technology},
number = TBD,
volume = 347,
place = {United States},
year = {Thu Nov 18 00:00:00 EST 2021},
month = {Thu Nov 18 00:00:00 EST 2021}
}
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Works referenced in this record:

Advances in microbial lignin degradation and its applications
journal, April 2019

Metabolic engineering of Pseudomonas putida for increased polyhydroxyalkanoate production from lignin
journal, January 2020

  • Salvachúa, Davinia; Rydzak, Thomas; Auwae, Raquel
  • Microbial Biotechnology, Vol. 13, Issue 1
  • DOI: 10.1111/1751-7915.13481

Improved value and carbon footprint by complete utilization of corncob lignocellulose
journal, September 2021

Glucose-Free cis , cis -Muconic Acid Production via New Metabolic Designs Corresponding to the Heterogeneity of Lignin
journal, November 2017

Revealing the structure and distribution changes of Eucalyptus lignin during the hydrothermal and alkaline pretreatments
journal, April 2017

Chemical Transformations of Poplar Lignin during Cosolvent Enhanced Lignocellulosic Fractionation Process
journal, May 2018

  • Meng, Xianzhi; Parikh, Aakash; Seemala, Bhogeswararao
  • ACS Sustainable Chemistry & Engineering, Vol. 6, Issue 7
  • DOI: 10.1021/acssuschemeng.8b01028

Acid Reactions of Lignin Models of β-5 Type
journal, January 1999

THF co-solvent enhances hydrocarbon fuel precursor yields from lignocellulosic biomass
journal, January 2013

  • Cai, Charles M.; Zhang, Taiying; Kumar, Rajeev
  • Green Chemistry, Vol. 15, Issue 11
  • DOI: 10.1039/c3gc41214h

Transforming biorefinery designs with ‘Plug-In Processes of Lignin’ to enable economic waste valorization
journal, June 2021

Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products
journal, February 2019

Bacterial enzymes involved in lignin degradation
journal, October 2016

Methylation-triggered fractionation of lignocellulosic biomass to afford cellulose-, hemicellulose-, and lignin-based functional polymers via click chemistry
journal, January 2020

  • Miki, Kentaro; Kamitakahara, Hiroshi; Yoshinaga, Arata
  • Green Chemistry, Vol. 22, Issue 9
  • DOI: 10.1039/D0GC00451K

Identifying and creating pathways to improve biological lignin valorization
journal, May 2019

Microbial polyhydroxyalkanoate production from lignin by Pseudomonas putida NX-1
journal, January 2021

Structural Characterization of Switchgrass Lignin after Ethanol Organosolv Pretreatment
journal, December 2011

  • Hu, Gang; Cateto, Carolina; Pu, Yunqiao
  • Energy & Fuels, Vol. 26, Issue 1
  • DOI: 10.1021/ef201477p

Pretreatment of Lodgepole Pine Killed by Mountain Pine Beetle Using the Ethanol Organosolv Process:  Fractionation and Process Optimization
journal, April 2007

  • Pan, Xuejun; Xie, Dan; Yu, Richard W.
  • Industrial & Engineering Chemistry Research, Vol. 46, Issue 8
  • DOI: 10.1021/ie061576l

Lignocellulosic biorefineries: The current state of challenges and strategies for efficient commercialization
journal, September 2021

  • Usmani, Zeba; Sharma, Minaxi; Awasthi, Abhishek Kumar
  • Renewable and Sustainable Energy Reviews, Vol. 148
  • DOI: 10.1016/j.rser.2021.111258

Discovery and Characterization of Heme Enzymes from Unsequenced Bacteria: Application to Microbial Lignin Degradation
journal, November 2011

  • Brown, Margaret E.; Walker, Mark C.; Nakashige, Toshiki G.
  • Journal of the American Chemical Society, Vol. 133, Issue 45
  • DOI: 10.1021/ja203972q

Production of itaconic acid from alkali pretreated lignin by dynamic two stage bioconversion
journal, April 2021

Fractionation of alkali-extracted lignin from steam-exploded stalk by gradient acid precipitation
journal, February 2013

Elucidating the mechanisms of enhanced lignin bioconversion by an alkali sterilization strategy
journal, January 2021

  • Zhao, Zhi-Min; Zhang, Shuyang; Meng, Xianzhi
  • Green Chemistry, Vol. 23, Issue 13
  • DOI: 10.1039/D1GC00911G

Synthesis, Characterization, and Utilization of a Lignin-Based Adsorbent for Effective Removal of Azo Dye from Aqueous Solution
journal, February 2020

A Multifunctional Cosolvent Pair Reveals Molecular Principles of Biomass Deconstruction
journal, July 2019

  • Patri, Abhishek S.; Mostofian, Barmak; Pu, Yunqiao
  • Journal of the American Chemical Society, Vol. 141, Issue 32
  • DOI: 10.1021/jacs.8b10242

Biorefineries in circular bioeconomy: A comprehensive review
journal, March 2020

Polyurethanes Based on Unmodified and Refined Technical Lignins: Correlation between Molecular Structure and Material Properties
journal, April 2021

Valorization of industrial lignin to value-added chemicals by chemical depolymerization and biological conversion
journal, March 2021

Deconstruction of biomass enabled by local demixing of cosolvents at cellulose and lignin surfaces
journal, July 2020

  • Pingali, Sai Venkatesh; Smith, Micholas Dean; Liu, Shih-Hsien
  • Proceedings of the National Academy of Sciences, Vol. 117, Issue 29
  • DOI: 10.1073/pnas.1922883117

Sustainability metrics of pretreatment processes in a waste derived lignocellulosic biomass biorefinery
journal, February 2020

Biotransformation of lignin: Mechanisms, applications and future work
journal, October 2019

  • Li, Xiang; Zheng, Yi
  • Biotechnology Progress, Vol. 36, Issue 1
  • DOI: 10.1002/btpr.2922

Cosolvent pretreatment in cellulosic biofuel production: effect of tetrahydrofuran-water on lignin structure and dynamics
journal, January 2016

  • Smith, Micholas Dean; Mostofian, Barmak; Cheng, Xiaolin
  • Green Chemistry, Vol. 18, Issue 5
  • DOI: 10.1039/C5GC01952D

Lipid Production from Dilute Alkali Corn Stover Lignin by Rhodococcus Strains
journal, January 2017

Fast Fractionation of Technical Lignins by Organic Cosolvents
journal, March 2018

Whole plant cell wall characterization using solution-state 2D NMR
journal, August 2012

Emerging Strategies for Modifying Lignin Chemistry to Enhance Biological Lignin Valorization
journal, August 2020

Co-solvent Pretreatment Reduces Costly Enzyme Requirements for High Sugar and Ethanol Yields from Lignocellulosic Biomass
journal, February 2015

Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of Rhodococci strains
journal, March 2019

Opportunities and challenges in biological lignin valorization
journal, December 2016

Use of ensiled biomass sorghum increases ionic liquid pretreatment efficiency and reduces biofuel production cost and carbon footprint
journal, January 2021

  • Magurudeniya, Harsha D.; Baral, Nawa Raj; Rodriguez, Alberto
  • Green Chemistry, Vol. 23, Issue 8
  • DOI: 10.1039/D0GC03260C

Improved enrichment factor calculations through principal component analysis: Examples from soils near breccia pipe uranium mines, Arizona, USA
journal, May 2019

Combinatorial pretreatment and fermentation optimization enabled a record yield on lignin bioconversion
journal, January 2018

Synergistic enzymatic and microbial lignin conversion
journal, January 2016

  • Zhao, Cheng; Xie, Shangxian; Pu, Yunqiao
  • Green Chemistry, Vol. 18, Issue 5
  • DOI: 10.1039/C5GC01955A

Development of a Rhodococcus opacus Cell Factory for Valorizing Lignin to Muconate
journal, January 2020

Bioconversion of hybrid poplar to ethanol and co-products using an organosolv fractionation process: Optimization of process yields
journal, January 2006

  • Pan, Xuejun; Gilkes, Neil; Kadla, John
  • Biotechnology and Bioengineering, Vol. 94, Issue 5, p. 851-861
  • DOI: 10.1002/bit.20905

Advanced Chemical Design for Efficient Lignin Bioconversion
journal, January 2017

Overcoming factors limiting high-solids fermentation of lignocellulosic biomass to ethanol
journal, October 2017

  • Nguyen, Thanh Yen; Cai, Charles M.; Kumar, Rajeev
  • Proceedings of the National Academy of Sciences, Vol. 114, Issue 44
  • DOI: 10.1073/pnas.1704652114

Physical structure changes of solid medium by steam explosion sterilization
journal, March 2016

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