Drought Drives Spatial Variation in the Millet Root Microbiome
Abstract
Efforts to boost crop yield and meet global food demands while striving to reach sustainability goals are hindered by the increasingly severe impacts of abiotic stress, such as drought. One strategy for alleviating drought stress in crops is to utilize rootassociated bacteria, yet knowledge concerning the relationship between plant hosts and their microbiomes during drought remain under-studied. One broad pattern that has recently been reported in a variety of monocot and dicot species from both native and agricultural environments, is the enrichment of Actinobacteria within the drought-stressed root microbiome. In order to better understand the causes of this phenomenon, we performed a series of experiments in millet plants to explore the roles of drought severity, drought localization, and root development in provoking Actinobacteria enrichment within the root endosphere. Through 16S rRNA ampliconbased sequencing, we demonstrate that the degree of drought is correlated with levels of Actinobacterial enrichment in four species of millet. Additionally, we demonstrate that the observed drought-induced enrichment of Actinobacteria occurs along the length of the root, but the response is localized to portions of the root experiencing drought. Finally, we demonstrate that Actinobacteria are depleted in the dead root tissue of Japanese millet, suggesting saprophytic activity ismore »
- Authors:
-
- Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology
- Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology; US Dept. of Agriculture (USDA), Albany, CA (United States). Agricultural Research Service (ARS). Plant Gene Expression Center
- Publication Date:
- Research Org.:
- Univ. of California, Oakland, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1802557
- Grant/Contract Number:
- SC0014081
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Plant Science
- Additional Journal Information:
- Journal Volume: 11; Journal ID: ISSN 1664-462X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Plant Sciences; plant microbiome; abiotic stress; root endosphere; drought; plant microbe interaction
Citation Formats
Simmons, Tuesday, Styer, Alexander B., Pierroz, Grady, Gonçalves, Antonio Pedro, Pasricha, Ramji, Hazra, Amrita B., Bubner, Patricia, and Coleman-Derr, Devin. Drought Drives Spatial Variation in the Millet Root Microbiome. United States: N. p., 2020.
Web. doi:10.3389/fpls.2020.00599.
Simmons, Tuesday, Styer, Alexander B., Pierroz, Grady, Gonçalves, Antonio Pedro, Pasricha, Ramji, Hazra, Amrita B., Bubner, Patricia, & Coleman-Derr, Devin. Drought Drives Spatial Variation in the Millet Root Microbiome. United States. https://doi.org/10.3389/fpls.2020.00599
Simmons, Tuesday, Styer, Alexander B., Pierroz, Grady, Gonçalves, Antonio Pedro, Pasricha, Ramji, Hazra, Amrita B., Bubner, Patricia, and Coleman-Derr, Devin. Thu .
"Drought Drives Spatial Variation in the Millet Root Microbiome". United States. https://doi.org/10.3389/fpls.2020.00599. https://www.osti.gov/servlets/purl/1802557.
@article{osti_1802557,
title = {Drought Drives Spatial Variation in the Millet Root Microbiome},
author = {Simmons, Tuesday and Styer, Alexander B. and Pierroz, Grady and Gonçalves, Antonio Pedro and Pasricha, Ramji and Hazra, Amrita B. and Bubner, Patricia and Coleman-Derr, Devin},
abstractNote = {Efforts to boost crop yield and meet global food demands while striving to reach sustainability goals are hindered by the increasingly severe impacts of abiotic stress, such as drought. One strategy for alleviating drought stress in crops is to utilize rootassociated bacteria, yet knowledge concerning the relationship between plant hosts and their microbiomes during drought remain under-studied. One broad pattern that has recently been reported in a variety of monocot and dicot species from both native and agricultural environments, is the enrichment of Actinobacteria within the drought-stressed root microbiome. In order to better understand the causes of this phenomenon, we performed a series of experiments in millet plants to explore the roles of drought severity, drought localization, and root development in provoking Actinobacteria enrichment within the root endosphere. Through 16S rRNA ampliconbased sequencing, we demonstrate that the degree of drought is correlated with levels of Actinobacterial enrichment in four species of millet. Additionally, we demonstrate that the observed drought-induced enrichment of Actinobacteria occurs along the length of the root, but the response is localized to portions of the root experiencing drought. Finally, we demonstrate that Actinobacteria are depleted in the dead root tissue of Japanese millet, suggesting saprophytic activity is not the main cause of observed shifts in drought-treated root microbiome structure. Collectively, these results help narrow the list of potential causes of drought-induced Actinobacterial enrichment in plant roots by showing that enrichment is dependent upon localized drought responses but not root developmental stage or root death.},
doi = {10.3389/fpls.2020.00599},
journal = {Frontiers in Plant Science},
number = ,
volume = 11,
place = {United States},
year = {Thu May 28 00:00:00 EDT 2020},
month = {Thu May 28 00:00:00 EDT 2020}
}
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