Inorganic N addition replaces N supplied to switchgrass ( Panicum virgatum ) by arbuscular mycorrhizal fungi
Abstract
Arbuscular mycorrhizal fungi (AMF) provide many benefits in agroecosystems including improved soil tilth, carbon sequestration, and water and nutrient transfer to plants. AMF are known to affect plant nitrogen (N) dynamics and transfer N to plants, but there have been few studies addressing whether the amount of N transferred to plants by AMF is agronomically relevant. We used δ15N natural abundance methods and δ15N mass balance equations to estimate the amount of plant N derived from AMF transfer in perennial grasses managed for bioenergy production under different N addition treatments (0, 56, and 196 kg N/ha). Differentiation of δ15N among plant, soil N, and AMF pools was higher than anticipated leading to calculations of 34–55% of plant N transferred by AMF in the treatments receiving no N addition to 6–22% of plant N transferred to plants in high-N addition treatments. AMF extra-radical hyphae biomass was significantly reduced in the high-N (196 kg N/ha) addition treatments, which was negatively correlated to enriched plant δ15N. Our results suggest that N addition decreases AMF N transfer to plants. When N was limiting to plant growth, AMF supplied agronomically significant amounts of plant N, and a higher proportion of overall plant N. Because differentiationmore »
- Authors:
-
- Univ. of Wisconsin, Madison, WI (United States); Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)
- Publication Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States); Battelle Memorial Institute, Columbus, OH (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1799678
- Grant/Contract Number:
- FC02-07ER64494; AC05-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Ecological Applications
- Additional Journal Information:
- Journal Volume: 30; Journal Issue: 2; Journal ID: ISSN 1051-0761
- Publisher:
- Ecological Society of America
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; Environmental Sciences & Ecology
Citation Formats
Jach‐Smith, Laura C., and Jackson, Randall D. Inorganic N addition replaces N supplied to switchgrass ( Panicum virgatum ) by arbuscular mycorrhizal fungi. United States: N. p., 2019.
Web. doi:10.1002/eap.2047.
Jach‐Smith, Laura C., & Jackson, Randall D. Inorganic N addition replaces N supplied to switchgrass ( Panicum virgatum ) by arbuscular mycorrhizal fungi. United States. https://doi.org/10.1002/eap.2047
Jach‐Smith, Laura C., and Jackson, Randall D. Sat .
"Inorganic N addition replaces N supplied to switchgrass ( Panicum virgatum ) by arbuscular mycorrhizal fungi". United States. https://doi.org/10.1002/eap.2047. https://www.osti.gov/servlets/purl/1799678.
@article{osti_1799678,
title = {Inorganic N addition replaces N supplied to switchgrass ( Panicum virgatum ) by arbuscular mycorrhizal fungi},
author = {Jach‐Smith, Laura C. and Jackson, Randall D.},
abstractNote = {Arbuscular mycorrhizal fungi (AMF) provide many benefits in agroecosystems including improved soil tilth, carbon sequestration, and water and nutrient transfer to plants. AMF are known to affect plant nitrogen (N) dynamics and transfer N to plants, but there have been few studies addressing whether the amount of N transferred to plants by AMF is agronomically relevant. We used δ15N natural abundance methods and δ15N mass balance equations to estimate the amount of plant N derived from AMF transfer in perennial grasses managed for bioenergy production under different N addition treatments (0, 56, and 196 kg N/ha). Differentiation of δ15N among plant, soil N, and AMF pools was higher than anticipated leading to calculations of 34–55% of plant N transferred by AMF in the treatments receiving no N addition to 6–22% of plant N transferred to plants in high-N addition treatments. AMF extra-radical hyphae biomass was significantly reduced in the high-N (196 kg N/ha) addition treatments, which was negatively correlated to enriched plant δ15N. Our results suggest that N addition decreases AMF N transfer to plants. When N was limiting to plant growth, AMF supplied agronomically significant amounts of plant N, and a higher proportion of overall plant N. Because differentiation between N pools was greater than expected, stable isotope measurements can be used to estimate N transfer to AMF plant hosts.},
doi = {10.1002/eap.2047},
journal = {Ecological Applications},
number = 2,
volume = 30,
place = {United States},
year = {Sat Nov 23 00:00:00 EST 2019},
month = {Sat Nov 23 00:00:00 EST 2019}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Roles of Arbuscular Mycorrhizas in Plant Nutrition and Growth: New Paradigms from Cellular to Ecosystem Scales
journal, June 2011
- Smith, Sally E.; Smith, F. Andrew
- Annual Review of Plant Biology, Vol. 62, Issue 1
Agroecology: the key role of arbuscular mycorrhizas in ecosystem services
journal, August 2010
- Gianinazzi, Silvio; Gollotte, Armelle; Binet, Marie-Noëlle
- Mycorrhiza, Vol. 20, Issue 8
Tradeoffs in Biomass and Nutrient Allocation in Prairies and Corn Managed for Bioenergy Production
journal, January 2012
- Jarchow, Meghann E.; Liebman, Matt
- Crop Science, Vol. 52, Issue 3
Assimilation and isotopic fractionation of nitrogen by mycorrhizal fungi
journal, August 2001
- Emmerton, K. S.; Callaghan, T. V.; Jones, H. E.
- New Phytologist, Vol. 151, Issue 2
Simulated Nitrogen Deposition Causes a Decline of Intra- and Extraradical Abundance of Arbuscular Mycorrhizal Fungi and Changes in Microbial Community Structure in Northern Hardwood Forests
journal, June 2010
- van Diepen, Linda T. A.; Lilleskov, Erik A.; Pregitzer, Kurt S.
- Ecosystems, Vol. 13, Issue 5
Resource limitation is a driver of local adaptation in mycorrhizal symbioses
journal, January 2010
- Johnson, N. C.; Wilson, G. W. T.; Bowker, M. A.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 5
Community traitscape of foliar nitrogen isotopes reveals N availability patterns in a tallgrass prairie
journal, February 2012
- Craine, Joseph M.; Towne, E. Gene; Ocheltree, Troy W.
- Plant and Soil, Vol. 356, Issue 1-2
Species-dependent partitioning of C and N stable isotopes between arbuscular mycorrhizal fungi and their C3 and C4 hosts
journal, March 2015
- Courty, Pierre-Emmanuel; Doubková, Pavla; Calabrese, Silvia
- Soil Biology and Biochemistry, Vol. 82
Measuring external mycelia production of ectomycorrhizal fungi in the field: the soil matrix matters
journal, July 2006
- Hendricks, Joseph J.; Mitchell, Robert J.; Kuehn, Kevin A.
- New Phytologist, Vol. 171, Issue 1
Carbon availability triggers fungal nitrogen uptake and transport in arbuscular mycorrhizal symbiosis
journal, January 2012
- Fellbaum, C. R.; Gachomo, E. W.; Beesetty, Y.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 7
Interspecific nutrient transfer in a tallgrass prairie plant community
journal, February 1996
- Walter, Laura E. Fischer; Hartnett, David C.; Hetrick, Barbara A. D.
- American Journal of Botany, Vol. 83, Issue 2
Nitrogen isotopes link mycorrhizal fungi and plants to nitrogen dynamics
journal, August 2012
- Hobbie, Erik A.; Högberg, Peter
- New Phytologist, Vol. 196, Issue 2
A meta-analysis of mycorrhizal responses to nitrogen, phosphorus, and atmospheric CO2 in field studies
journal, July 2004
- Treseder, Kathleen K.
- New Phytologist, Vol. 164, Issue 2
Plant–microbial linkages and ecosystem nitrogen retention: lessons for sustainable agriculture
journal, May 2012
- de Vries, Franciska T.; Bardgett, Richard D.
- Frontiers in Ecology and the Environment, Vol. 10, Issue 8
Soil biota enhance agricultural sustainability by improving crop yield, nutrient uptake and reducing nitrogen leaching losses
journal, October 2014
- Bender, S. Franz; van der Heijden, Marcel G. A.
- Journal of Applied Ecology, Vol. 52, Issue 1
Global Distributions of Arbuscular Mycorrhizal Fungi
journal, March 2006
- Treseder, Kathleen K.; Cross, Alison
- Ecosystems, Vol. 9, Issue 2
Depleted 15N in hydrolysable-N of arctic soils and its implication for mycorrhizal fungi–plant interaction
journal, September 2009
- Yano, Y.; Shaver, G. R.; Giblin, A. E.
- Biogeochemistry, Vol. 97, Issue 2-3
13 C and 15 N isotopic fractionation in trees, soils and fungi in a natural forest stand and a Norway spruce plantation
journal, May 2007
- Zeller, Bernd; Brechet, Claude; Maurice, Jean-Paul
- Annals of Forest Science, Vol. 64, Issue 4
Correlations between foliar δ15N and nitrogen concentrations may indicate plant-mycorrhizal interactions
journal, February 2000
- Hobbie, E. A.; Macko, S. A.; Williams, M.
- Oecologia, Vol. 122, Issue 2
Biomass Production in Switchgrass across the United States: Database Description and Determinants of Yield
journal, January 2010
- Wullschleger, S. D.; Davis, E. B.; Borsuk, M. E.
- Agronomy Journal, Vol. 102, Issue 4
Tracking the carbon source of arbuscular mycorrhizal fungi colonizing C3 and C4 plants using carbon isotope ratios (δ13C)
journal, March 2013
- Walder, Florian; Niemann, Helge; Lehmann, Moritz F.
- Soil Biology and Biochemistry, Vol. 58
15 N IN SYMBIOTIC FUNGI AND PLANTS ESTIMATES NITROGEN AND CARBON FLUX RATES IN ARCTIC TUNDRA
journal, April 2006
- Hobbie, John E.; Hobbie, Erik A.
- Ecology, Vol. 87, Issue 4
Nitrogen transfer in the arbuscular mycorrhizal symbiosis
journal, June 2005
- Govindarajulu, Manjula; Pfeffer, Philip E.; Jin, Hairu
- Nature, Vol. 435, Issue 7043
Socialism in soil? The importance of mycorrhizal fungal networks for facilitation in natural ecosystems
journal, November 2009
- van der Heijden, Marcel G. A.; Horton, Thomas R.
- Journal of Ecology, Vol. 97, Issue 6
Controls over mycorrhizal uptake of organic nitrogen
journal, May 2010
- Talbot, J. M.; Treseder, K. K.
- Pedobiologia, Vol. 53, Issue 3
Ecological interpretations of nitrogen isotope ratios of terrestrial plants and soils
journal, June 2015
- Craine, Joseph M.; Brookshire, E. N. J.; Cramer, Michael D.
- Plant and Soil, Vol. 396, Issue 1-2
The Vegetation N:P Ratio: a New Tool to Detect the Nature of Nutrient Limitation
journal, December 1996
- Koerselman, Willem; Meuleman, Arthur F. M.
- The Journal of Applied Ecology, Vol. 33, Issue 6
Nitrogen in Agriculture: Balancing the Cost of an Essential Resource
journal, November 2009
- Robertson, G. Philip; Vitousek, Peter M.
- Annual Review of Environment and Resources, Vol. 34, Issue 1
Insights into root growth, function, and mycorrhizal abundance from chemical and isotopic data across root orders
journal, October 2012
- Ouimette, Andrew; Guo, Dali; Hobbie, Erik
- Plant and Soil, Vol. 367, Issue 1-2
Nitrogen input mediates the effect of free-air CO 2 enrichment on mycorrhizal fungal abundance: MYCORRHIZAS AND ELEVATED CO 2
journal, September 2004
- Staddon, Philip L.; Jakobsen, Iver; Blum, Herbert
- Global Change Biology, Vol. 10, Issue 10
Hyphal N transport by a vesicular-arbuscular mycorrhizal fungus associated with cucumber grown at three nitrogen levels
journal, March 1994
- Johansen, A.; Jakobsen, I.; Jensen, E. S.
- Plant and Soil, Vol. 160, Issue 1
N addition undermines N supplied by arbuscular mycorrhizal fungi to native perennial grasses
journal, January 2018
- Jach-Smith, Laura C.; Jackson, Randall D.
- Soil Biology and Biochemistry, Vol. 116
Global patterns of foliar nitrogen isotopes and their relationships with climate, mycorrhizal fungi, foliar nutrient concentrations, and nitrogen availability
journal, September 2009
- Craine, Joseph M.; Elmore, Andrew J.; Aidar, Marcos P. M.
- New Phytologist, Vol. 183, Issue 4
Agricultural practices indirectly influence plant productivity and ecosystem services through effects on soil biota
journal, October 2014
- Köhl, Luise; Oehl, Fritz; van der Heijden, Marcel G. A.
- Ecological Applications, Vol. 24, Issue 7
Nitrogen isotope fractionation during N uptake via arbuscular mycorrhizal and ectomycorrhizal fungi into grey alder
journal, October 2016
- Schweiger, Peter F.
- Journal of Plant Physiology, Vol. 205
The mycorrhizal contribution to plant productivity, plant nutrition and soil structure in experimental grassland
journal, December 2006
- van der Heijden, Marcel G. A.; Streitwolf-Engel, Ruth; Riedl, Ralph
- New Phytologist, Vol. 172, Issue 4
Nitrogen availability and colonization by mycorrhizal fungi correlate with nitrogen isotope patterns in plants
journal, January 2003
- Hobbie, Erik A.; Colpaert, Jan V.
- New Phytologist, Vol. 157, Issue 1
Estimation of the biomass and seasonal growth of external mycelium of ectomycorrhizal fungi in the field
journal, September 2001
- Wallander, Hakan; Nilsson, Lars Ola; Hagerberg, David
- New Phytologist, Vol. 151, Issue 3
Networks of power and influence: the role of mycorrhizal mycelium in controlling plant communities and agroecosystem functioning
journal, August 2004
- Leake, Jonathan; Johnson, David; Donnelly, Damian
- Canadian Journal of Botany, Vol. 82, Issue 8
Nitrogen conservation decreases with fertilizer addition in two perennial grass cropping systems for bioenergy
journal, June 2015
- Jach-Smith, Laura C.; Jackson, Randall D.
- Agriculture, Ecosystems & Environment, Vol. 204
Stable Isotopes in Plant Ecology
journal, November 2002
- Dawson, Todd E.; Mambelli, Stefania; Plamboeck, Agneta H.
- Annual Review of Ecology and Systematics, Vol. 33, Issue 1
Controls of isotopic patterns in saprotrophic and ectomycorrhizal fungi
journal, May 2012
- Hobbie, Erik A.; Sánchez, Fernando S.; Rygiewicz, Paul T.
- Soil Biology and Biochemistry, Vol. 48
Arbuscular mycorrhiza and nitrogen: implications for individual plants through to ecosystems
journal, June 2014
- Hodge, Angela; Storer, Kate
- Plant and Soil, Vol. 386, Issue 1-2
Natural Abundance of 15N in Nitrogen-Limited Forests and Tundra Can Estimate Nitrogen Cycling Through Mycorrhizal Fungi: A Review
journal, May 2008
- Hobbie, E. A.; Hobbie, J. E.
- Ecosystems, Vol. 11, Issue 5
Associative nitrogen fixation (ANF) in switchgrass (Panicum virgatum) across a nitrogen input gradient
journal, June 2018
- Roley, Sarah S.; Duncan, David S.; Liang, Di
- PLOS ONE, Vol. 13, Issue 6
Nitrogen Uptake by Trees and Mycorrhizal Fungi in a Successional Northern Temperate Forest: Insights from Multiple Isotopic Methods
journal, January 2013
- Nave, Lucas E.; Nadelhoffer, Knute J.; Le Moine, James M.
- Ecosystems, Vol. 16, Issue 4
Nutritional ecology of arbuscular mycorrhizal fungi
journal, November 2010
- Hodge, A.; Helgason, T.; Fitter, A. H.
- Fungal Ecology, Vol. 3, Issue 4
Arbuscular mycorrhiza and soil nitrogen cycling
journal, March 2012
- Veresoglou, Stavros D.; Chen, Baodong; Rillig, Matthias C.
- Soil Biology and Biochemistry, Vol. 46
The biogeochemistry of bioenergy landscapes: carbon, nitrogen, and water considerations
journal, June 2011
- Robertson, G. Philip; Hamilton, Stephen K.; Del Grosso, Stephen J.
- Ecological Applications, Vol. 21, Issue 4
Mycorrhizal-mediated nitrogen acquisition in switchgrass under elevated temperatures and N enrichment
journal, October 2011
- Schroeder-Moreno, Michelle S.; Greaver, Tara L.; Wang, Shuxin
- GCB Bioenergy, Vol. 4, Issue 3
Increasing plant use of organic nitrogen with elevation is reflected in nitrogen uptake rates and ecosystem δ 15 N
journal, April 2011
- Averill, Colin; Finzi, Adrien
- Ecology, Vol. 92, Issue 4
Nitrogen conservation strategies of cranberry plants and ericoid mycorrhizal fungi in an agroecosystem
journal, November 2008
- Stackpoole, Sarah M.; Workmaster, Beth Ann A.; Jackson, Randall D.
- Soil Biology and Biochemistry, Vol. 40, Issue 11
Assessing the Nitrogen-15 Concentration of Plant-Available Soil Nitrogen
journal, December 2004
- Hendricks, Joseph J.; Mitchell, Robert J.; Green, Kevin M.
- Communications in Soil Science and Plant Analysis, Vol. 35, Issue 9-10
Nitrogen Enrichment Alters Mycorrhizal Allocation at five Mesic to Semiarid Grasslands
journal, July 2003
- Johnson, Nancy Collins; Rowland, Diane L.; Corkidi, Lea
- Ecology, Vol. 84, Issue 7
Arbuscular mycorrhizae and terrestrial ecosystem processes
journal, August 2004
- Rillig, Matthias C.
- Ecology Letters, Vol. 7, Issue 8
The Biology and Agronomy of Switchgrass for Biofuels
journal, September 2005
- Parrish, David J.; Fike, John H.
- Critical Reviews in Plant Sciences, Vol. 24, Issue 5-6
Data from: Inorganic N addition replaces N supplied to switchgrass (Panicum virgatum) by arbuscular mycorrhizal fungi
dataset, January 2019
- Jach Smith, Laura
- Dryad
Data from: Inorganic N addition replaces N supplied to switchgrass (Panicum virgatum) by arbuscular mycorrhizal fungi
dataset, January 2019
- Jach Smith, Laura
- Dryad
Works referencing / citing this record:
Data from: Inorganic N addition replaces N supplied to switchgrass (Panicum virgatum) by arbuscular mycorrhizal fungi
dataset, January 2019
- Jach Smith, Laura
- Dryad
Data from: Inorganic N addition replaces N supplied to switchgrass (Panicum virgatum) by arbuscular mycorrhizal fungi
dataset, January 2019
- Jach Smith, Laura
- Dryad