Regulatory Tools for Controlling Gene Expression in Cyanobacteria
Abstract
Cyanobacteria are attractive hosts for converting carbon dioxide and sunlight into desirable chemical products. To engineer these organisms and manipulate their metabolic pathways, the biotechnology community has developed genetic tools to control gene expression. Many native cyanobacterial promoters and related sequence elements have been used to regulate genes of interest, and heterologous tools that use non-native small molecules to induce gene expression have been demonstrated. Overall, IPTG-based induction systems seem to be leaky and initially demonstrate small dynamic ranges in cyanobacteria. Consequently, a variety of other induction systems have been optimized to enable tighter control of gene expression. Tools require significant optimization because they function quite differently in cyanobacteria when compared to analogous use in model heterotrophs. We hypothesize that these differences are due to fundamental differences in physiology between organisms. This review is not intended to summarize all known products made in cyanobacteria nor the performance (titer, rate, yield) of individual strains, but instead will focus on the genetic tools and the inherent aspects of cellular physiology that influence gene expression in cyanobacteria.
- Authors:
-
- Univ. of Wisconsin, Madison, WI (United States). Dept. of Chemical and Biological Engineering, and Microbiology Doctoral Training Program
- Publication Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1515186
- Grant/Contract Number:
- SC0010329
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Advances in Experimental Medicine and Biology
- Additional Journal Information:
- Journal Volume: 1080; Journal ID: ISSN 0065-2598
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Promoter; Induction system; Termination; Genome editing
Citation Formats
Gordon, Gina C., and Pfleger, Brian F. Regulatory Tools for Controlling Gene Expression in Cyanobacteria. United States: N. p., 2018.
Web. doi:10.1007/978-981-13-0854-3_12.
Gordon, Gina C., & Pfleger, Brian F. Regulatory Tools for Controlling Gene Expression in Cyanobacteria. United States. https://doi.org/10.1007/978-981-13-0854-3_12
Gordon, Gina C., and Pfleger, Brian F. Thu .
"Regulatory Tools for Controlling Gene Expression in Cyanobacteria". United States. https://doi.org/10.1007/978-981-13-0854-3_12. https://www.osti.gov/servlets/purl/1515186.
@article{osti_1515186,
title = {Regulatory Tools for Controlling Gene Expression in Cyanobacteria},
author = {Gordon, Gina C. and Pfleger, Brian F.},
abstractNote = {Cyanobacteria are attractive hosts for converting carbon dioxide and sunlight into desirable chemical products. To engineer these organisms and manipulate their metabolic pathways, the biotechnology community has developed genetic tools to control gene expression. Many native cyanobacterial promoters and related sequence elements have been used to regulate genes of interest, and heterologous tools that use non-native small molecules to induce gene expression have been demonstrated. Overall, IPTG-based induction systems seem to be leaky and initially demonstrate small dynamic ranges in cyanobacteria. Consequently, a variety of other induction systems have been optimized to enable tighter control of gene expression. Tools require significant optimization because they function quite differently in cyanobacteria when compared to analogous use in model heterotrophs. We hypothesize that these differences are due to fundamental differences in physiology between organisms. This review is not intended to summarize all known products made in cyanobacteria nor the performance (titer, rate, yield) of individual strains, but instead will focus on the genetic tools and the inherent aspects of cellular physiology that influence gene expression in cyanobacteria.},
doi = {10.1007/978-981-13-0854-3_12},
journal = {Advances in Experimental Medicine and Biology},
number = ,
volume = 1080,
place = {United States},
year = {Thu Aug 09 00:00:00 EDT 2018},
month = {Thu Aug 09 00:00:00 EDT 2018}
}
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Works referencing / citing this record:
Metabolic engineering of a fast-growing cyanobacterium Synechococcus elongatus PCC 11801 for photoautotrophic production of succinic acid
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