skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: New Applications of Synthetic Biology Tools for Cyanobacterial Metabolic Engineering

Abstract

Cyanobacteria are promising microorganisms for sustainable biotechnologies, yet unlocking their potential requires radical re-engineering and application of cutting-edge synthetic biology techniques. In recent years, the available devices and strategies for modifying cyanobacteria have been increasing, including advances in the design of genetic promoters, ribosome binding sites, riboswitches, reporter proteins, modular vector systems, and markerless selection systems. Because of these new toolkits, cyanobacteria have been successfully engineered to express heterologous pathways for the production of a wide variety of valuable compounds. Cyanobacterial strains with the potential to be used in real-world applications will require the refinement of genetic circuits used to express the heterologous pathways and development of accurate models that predict how these pathways can be best integrated into the larger cellular metabolic network. Herein, we review advances that have been made to translate synthetic biology tools into cyanobacterial model organisms and summarize experimental and in silico strategies that have been employed to increase their bioproduction potential. Despite the advances in synthetic biology and metabolic engineering during the last years, it is clear that still further improvements are required if cyanobacteria are to be competitive with heterotrophic microorganisms for the bioproduction of added-value compounds.

Authors:
; ;
Publication Date:
Research Org.:
Michigan State Univ., East Lansing, MI (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1496889
Alternate Identifier(s):
OSTI ID: 1610156
Grant/Contract Number:  
FG02-91ER20021
Resource Type:
Published Article
Journal Name:
Frontiers in Bioengineering and Biotechnology
Additional Journal Information:
Journal Name: Frontiers in Bioengineering and Biotechnology Journal Volume: 7; Journal ID: ISSN 2296-4185
Publisher:
Frontiers Research Foundation
Country of Publication:
Switzerland
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Biotechnology & Applied Microbiology; cyanobacteria; metabolic engineering; synthetic biology; genome scale models; photosynthesis

Citation Formats

Santos-Merino, María, Singh, Amit K., and Ducat, Daniel C. New Applications of Synthetic Biology Tools for Cyanobacterial Metabolic Engineering. Switzerland: N. p., 2019. Web. https://doi.org/10.3389/fbioe.2019.00033.
Santos-Merino, María, Singh, Amit K., & Ducat, Daniel C. New Applications of Synthetic Biology Tools for Cyanobacterial Metabolic Engineering. Switzerland. https://doi.org/10.3389/fbioe.2019.00033
Santos-Merino, María, Singh, Amit K., and Ducat, Daniel C. Wed . "New Applications of Synthetic Biology Tools for Cyanobacterial Metabolic Engineering". Switzerland. https://doi.org/10.3389/fbioe.2019.00033.
@article{osti_1496889,
title = {New Applications of Synthetic Biology Tools for Cyanobacterial Metabolic Engineering},
author = {Santos-Merino, María and Singh, Amit K. and Ducat, Daniel C.},
abstractNote = {Cyanobacteria are promising microorganisms for sustainable biotechnologies, yet unlocking their potential requires radical re-engineering and application of cutting-edge synthetic biology techniques. In recent years, the available devices and strategies for modifying cyanobacteria have been increasing, including advances in the design of genetic promoters, ribosome binding sites, riboswitches, reporter proteins, modular vector systems, and markerless selection systems. Because of these new toolkits, cyanobacteria have been successfully engineered to express heterologous pathways for the production of a wide variety of valuable compounds. Cyanobacterial strains with the potential to be used in real-world applications will require the refinement of genetic circuits used to express the heterologous pathways and development of accurate models that predict how these pathways can be best integrated into the larger cellular metabolic network. Herein, we review advances that have been made to translate synthetic biology tools into cyanobacterial model organisms and summarize experimental and in silico strategies that have been employed to increase their bioproduction potential. Despite the advances in synthetic biology and metabolic engineering during the last years, it is clear that still further improvements are required if cyanobacteria are to be competitive with heterotrophic microorganisms for the bioproduction of added-value compounds.},
doi = {10.3389/fbioe.2019.00033},
journal = {Frontiers in Bioengineering and Biotechnology},
number = ,
volume = 7,
place = {Switzerland},
year = {2019},
month = {2}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.3389/fbioe.2019.00033

Citation Metrics:
Cited by: 11 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Network reconstruction of platelet metabolism identifies metabolic signature for aspirin resistance
journal, January 2014

  • Thomas, Alex; Rahmanian, Sorena; Bordbar, Aarash
  • Scientific Reports, Vol. 4, Issue 1
  • DOI: 10.1038/srep03925

Dynamic Localization of the Cyanobacterial Circadian Clock Proteins
journal, August 2014


Metabolic model of Synechococcus sp. PCC 7002: Prediction of flux distribution and network modification for enhanced biofuel production
journal, August 2016


Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria
journal, December 2016

  • Ungerer, Justin; Pakrasi, Himadri B.
  • Scientific Reports, Vol. 6, Issue 1
  • DOI: 10.1038/srep39681

Carbon recycling by cyanobacteria: improving CO2 fixation through chemical production
journal, August 2017

  • Zhang, Angela; Carroll, Austin L.; Atsumi, Shota
  • FEMS Microbiology Letters, Vol. 364, Issue 16
  • DOI: 10.1093/femsle/fnx165

Metabolic engineering of cyanobacteria for the synthesis of commodity products
journal, June 2015

  • Angermayr, S. Andreas; Gorchs Rovira, Aleix; Hellingwerf, Klaas J.
  • Trends in Biotechnology, Vol. 33, Issue 6
  • DOI: 10.1016/j.tibtech.2015.03.009

Toward systems metabolic engineering in cyanobacteria: Opportunities and bottlenecks
journal, May 2013

  • Nogales, Juan; Gudmundsson, Steinn; Thiele, Ines
  • Bioengineered, Vol. 4, Issue 3
  • DOI: 10.4161/bioe.22792

Standardization in synthetic biology: an engineering discipline coming of age
journal, September 2017


Evidence for an NIH shift in oxidation of naphthalene by the marine cyanobacterium Oscillatoria sp. strain JCM.
journal, January 1992


Reconstruction and verification of a genome-scale metabolic model for Synechocystis sp. PCC6803
journal, September 2011

  • Yoshikawa, Katsunori; Kojima, Yuta; Nakajima, Tsubasa
  • Applied Microbiology and Biotechnology, Vol. 92, Issue 2
  • DOI: 10.1007/s00253-011-3559-x

Metabolic engineering of the pentose phosphate pathway for enhanced limonene production in the cyanobacterium Synechocysti s sp. PCC 6803
journal, December 2017


KEGG: Kyoto Encyclopedia of Genes and Genomes
journal, January 2000

  • Kanehisa, Minoru; Goto, Susumu
  • Nucleic Acids Research, Vol. 28, Issue 1, p. 27-30
  • DOI: 10.1093/nar/28.1.27

Reconstruction and Comparison of the Metabolic Potential of Cyanobacteria Cyanothece sp. ATCC 51142 and Synechocystis sp. PCC 6803
journal, October 2012


High-Frequency Gene Replacement in Cyanobacteria Using a Heterologous rps12 Gene
journal, March 2004

  • Takahama, Kazutaka; Matsuoka, Masayoshi; Nagahama, Kazuhiro
  • Plant and Cell Physiology, Vol. 45, Issue 3
  • DOI: 10.1093/pcp/pch041

Cyanobacteria and microalgae: A positive prospect for biofuels
journal, November 2011


CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production
journal, November 2016


Sustained photosynthetic conversion of CO2 to ethylene in recombinant cyanobacterium Synechocystis 6803
journal, January 2012

  • Ungerer, Justin; Tao, Ling; Davis, Mark
  • Energy & Environmental Science, Vol. 5, Issue 10, p. 8998-9006
  • DOI: 10.1039/c2ee22555g

Rerouting Carbon Flux To Enhance Photosynthetic Productivity
journal, February 2012

  • Ducat, Daniel C.; Avelar-Rivas, J. Abraham; Way, Jeffrey C.
  • Applied and Environmental Microbiology, Vol. 78, Issue 8
  • DOI: 10.1128/AEM.07901-11

Limitation of Photosynthesis by Carbon Metabolism: II. O
journal, August 1986

  • Sharkey, Thomas D.; Stitt, Mark; Heineke, Dieter
  • Plant Physiology, Vol. 81, Issue 4
  • DOI: 10.1104/pp.81.4.1123

Exploring native genetic elements as plug-in tools for synthetic biology in the cyanobacterium Synechocystis sp. PCC 6803
journal, March 2018


Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in Synechococcus sp. PCC 7002
journal, June 2017

  • Vogel, Anne Ilse Maria; Lale, Rahmi; Hohmann-Marriott, Martin Frank
  • Journal of Biological Engineering, Vol. 11, Issue 1
  • DOI: 10.1186/s13036-017-0061-8

Three factors underlying incorrect in silico predictions of essential metabolic genes
journal, February 2008


Use of filamentous cyanobacteria for biodegradation of organic pollutants.
journal, January 1995


Flux coupling and transcriptional regulation within the metabolic network of the photosynthetic bacterium Synechocystis sp. PCC6803
journal, January 2011

  • Montagud, Arnau; Zelezniak, Aleksej; Navarro, Emilio
  • Biotechnology Journal, Vol. 6, Issue 3
  • DOI: 10.1002/biot.201000109

Circadian gating of cell division in cyanobacteria growing with average doubling times of less than 24 hours.
journal, September 1996

  • Mori, T.; Binder, B.; Johnson, C. H.
  • Proceedings of the National Academy of Sciences, Vol. 93, Issue 19
  • DOI: 10.1073/pnas.93.19.10183

Developing a Cas9‐based tool to engineer native plasmids in Synechocystis sp. PCC 6803
journal, June 2018

  • Xiao, Yi; Wang, Shaojie; Rommelfanger, Sarah
  • Biotechnology and Bioengineering, Vol. 115, Issue 9
  • DOI: 10.1002/bit.26747

Efficient Multiplex Genome Editing Induces Precise, and Self-Ligated Type Mutations in Tomato Plants
journal, July 2018


Precise and reliable gene expression via standard transcription and translation initiation elements
journal, March 2013

  • Mutalik, Vivek K.; Guimaraes, Joao C.; Cambray, Guillaume
  • Nature Methods, Vol. 10, Issue 4
  • DOI: 10.1038/nmeth.2404

Cyanobacterial Evolution: Fresh Insight into Ancient Questions
journal, March 2015


Transformation in the cyanobacterium Synechocystis sp. 6803
journal, April 1982


Natural Product Biosynthetic Diversity and Comparative Genomics of the Cyanobacteria
journal, October 2015


Theophylline-Dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC 7942
journal, September 2013

  • Nakahira, Yoichi; Ogawa, Atsushi; Asano, Hiroyuki
  • Plant and Cell Physiology, Vol. 54, Issue 10
  • DOI: 10.1093/pcp/pct115

Cyanobacterial conversion of carbon dioxide to 2,3-butanediol
journal, January 2013

  • Oliver, John W. K.; Machado, Iara M. P.; Yoneda, Hisanari
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 4
  • DOI: 10.1073/pnas.1213024110

Tracking, tuning, and terminating microbial physiology using synthetic riboregulators
journal, August 2010

  • Callura, J. M.; Dwyer, D. J.; Isaacs, F. J.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 36
  • DOI: 10.1073/pnas.1009747107

The plasticity of cyanobacterial carbon metabolism
journal, December 2017


Flux Balance Analysis of Cyanobacterial Metabolism: The Metabolic Network of Synechocystis sp. PCC 6803
journal, June 2013


Stoichiometric Representation of Gene–Protein–Reaction Associations Leverages Constraint-Based Analysis from Reaction to Gene-Level Phenotype Prediction
journal, October 2016


Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803
journal, November 2016


Construction of a Genome-Scale Metabolic Model of Arthrospira platensis NIES-39 and Metabolic Design for Cyanobacterial Bioproduction
journal, December 2015


Cyanobacteria: Promising biocatalysts for sustainable chemical production
journal, October 2017

  • Knoot, Cory J.; Ungerer, Justin; Wangikar, Pramod P.
  • Journal of Biological Chemistry, Vol. 293, Issue 14
  • DOI: 10.1074/jbc.R117.815886

Genetic tools for cyanobacteria
journal, January 2002


Generation and Evaluation of a Genome-Scale Metabolic Network Model of Synechococcus elongatus PCC7942
journal, August 2014

  • Triana, Julián; Montagud, Arnau; Siurana, Maria
  • Metabolites, Vol. 4, Issue 3
  • DOI: 10.3390/metabo4030680

MetaCyc: a multiorganism database of metabolic pathways and enzymes
journal, January 2006


Isobutanol production as an alternative metabolic sink to rescue the growth deficiency of the glycogen mutant of Synechococcus elongatus PCC 7942
journal, March 2014


Flux balance analysis of photoautotrophic metabolism: Uncovering new biological details of subsystems involved in cyanobacterial photosynthesis
journal, April 2017

  • Qian, Xiao; Kim, Min Kyung; Kumaraswamy, G. Kenchappa
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1858, Issue 4
  • DOI: 10.1016/j.bbabio.2016.12.007

A tightly inducible riboswitch system in <i>Synechocystis</i> sp. PCC 6803
journal, January 2016

  • Ohbayashi, Ryudo; Akai, Hideto; Yoshikawa, Hirofumi
  • The Journal of General and Applied Microbiology, Vol. 62, Issue 3
  • DOI: 10.2323/jgam.2016.02.002

Circadian orchestration of gene expression in cyanobacteria.
journal, June 1995

  • Liu, Y.; Tsinoremas, N. F.; Johnson, C. H.
  • Genes & Development, Vol. 9, Issue 12
  • DOI: 10.1101/gad.9.12.1469

The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST)
journal, November 2013

  • Overbeek, Ross; Olson, Robert; Pusch, Gordon D.
  • Nucleic Acids Research, Vol. 42, Issue D1
  • DOI: 10.1093/nar/gkt1226

Cyanobacteria as a Platform for Biofuel Production
journal, January 2013

  • Nozzi, Nicole E.; Oliver, John W. K.; Atsumi, Shota
  • Frontiers in Bioengineering and Biotechnology, Vol. 1
  • DOI: 10.3389/fbioe.2013.00007

Riboswitch engineering — making the all-important second and third steps
journal, February 2015


Genetic tools for advancement of Synechococcus sp. PCC 7002 as a cyanobacterial chassis
journal, November 2016

  • Ruffing, Anne M.; Jensen, Travis J.; Strickland, Lucas M.
  • Microbial Cell Factories, Vol. 15, Issue 1
  • DOI: 10.1186/s12934-016-0584-6

Synthetic Biology Toolbox for Controlling Gene Expression in the Cyanobacterium Synechococcus sp. strain PCC 7002
journal, September 2014

  • Markley, Andrew L.; Begemann, Matthew B.; Clarke, Ryan E.
  • ACS Synthetic Biology, Vol. 4, Issue 5
  • DOI: 10.1021/sb500260k

Grand Challenges in Synthetic Biology to be Accomplished
journal, January 2013


A glutamine riboswitch is a key element for the regulation of glutamine synthetase in cyanobacteria
journal, August 2018

  • Klähn, Stephan; Bolay, Paul; Wright, Patrick R.
  • Nucleic Acids Research
  • DOI: 10.1093/nar/gky709

Metabolism of monocrotophos and quinalphos by algae isolated from soil
journal, August 1987

  • Megharaj, M.; Venkateswarlu, K.; Rao, A. S.
  • Bulletin of Environmental Contamination and Toxicology, Vol. 39, Issue 2
  • DOI: 10.1007/BF01689414

Oxidation of Naphthalene by Cyanobacteria and Microalgae
journal, February 1980


Cyanobacteria: Photoautotrophic Microbial Factories for the Sustainable Synthesis of Industrial Products
journal, January 2015

  • Lau, Nyok-Sean; Matsui, Minami; Abdullah, Amirul Al-Ashraf
  • BioMed Research International, Vol. 2015
  • DOI: 10.1155/2015/754934

CRISPR-Based Technologies for Metabolic Engineering in Cyanobacteria
journal, October 2018


Algal oxidation of aromatic hydrocarbons: Formation of 1-naphthol from naphthalene by Agmenellum quadruplicatum, strain PR-6
journal, May 1979

  • Cerniglia, Carl E.; Gibson, David T.; Baalen, Chase Van
  • Biochemical and Biophysical Research Communications, Vol. 88, Issue 1
  • DOI: 10.1016/0006-291X(79)91695-4

Reconstruction and analysis of genome-scale metabolic model of a photosynthetic bacterium
journal, November 2010

  • Montagud, Arnau; Navarro, Emilio; Fernández de Córdoba, Pedro
  • BMC Systems Biology, Vol. 4, Issue 1
  • DOI: 10.1186/1752-0509-4-156

Flipping Riboswitches
journal, July 2006


Engineered cyanobacteria: Teaching an old bug new tricks
journal, May 2011


Flux balance analysis indicates that methane is the lowest cost feedstock for microbial cell factories
journal, December 2017


Sink regulation of photosynthesis
journal, July 2001


Nitrogen Control in Cyanobacteria
journal, January 2001


Computational metabolic engineering strategies for growth-coupled biofuel production by Synechocystis
journal, December 2016


A Genetic Toolbox for Modulating the Expression of Heterologous Genes in the Cyanobacterium Synechocystis sp. PCC 6803
journal, August 2017


Cas9 versus Cas12a/Cpf1: Structure-function comparisons and implications for genome editing
journal, May 2018

  • Swarts, Daan C.; Jinek, Martin
  • Wiley Interdisciplinary Reviews: RNA, Vol. 9, Issue 5
  • DOI: 10.1002/wrna.1481

Programming cells by multiplex genome engineering and accelerated evolution
journal, July 2009

  • Wang, Harris H.; Isaacs, Farren J.; Carr, Peter A.
  • Nature, Vol. 460, Issue 7257, p. 894-898
  • DOI: 10.1038/nature08187

A state of the art of metabolic networks of unicellular microalgae and cyanobacteria for biofuel production
journal, July 2015


Unique attributes of cyanobacterial metabolism revealed by improved genome-scale metabolic modeling and essential gene analysis
journal, December 2016

  • Broddrick, Jared T.; Rubin, Benjamin E.; Welkie, David G.
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 51
  • DOI: 10.1073/pnas.1613446113

Installing extra bicarbonate transporters in the cyanobacterium Synechocystis sp. PCC6803 enhances biomass production
journal, May 2015


The Bacillus BioBrick Box 2.0: expanding the genetic toolbox for the standardized work with Bacillus subtilis
journal, November 2017


Development of SyneBrick Vectors As a Synthetic Biology Platform for Gene Expression in Synechococcus elongatus PCC 7942
journal, March 2017


Live-cell imaging of cyanobacteria
journal, November 2014


Use of Degradation Tags To Control Protein Levels in the Cyanobacterium Synechocystis sp. Strain PCC 6803
journal, February 2013

  • Landry, Brian P.; Stöckel, Jana; Pakrasi, Himadri B.
  • Applied and Environmental Microbiology, Vol. 79, Issue 8
  • DOI: 10.1128/AEM.03741-12

Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering
journal, June 2011

  • Quintana, Naira; Van der Kooy, Frank; Van de Rhee, Miranda D.
  • Applied Microbiology and Biotechnology, Vol. 91, Issue 3
  • DOI: 10.1007/s00253-011-3394-0

Cyanobacteria: A Precious Bio-resource in Agriculture, Ecosystem, and Environmental Sustainability
journal, April 2016


Evaluation of promoters and ribosome binding sites for biotechnological applications in the unicellular cyanobacterium Synechocystis sp. PCC 6803
journal, November 2016

  • Englund, Elias; Liang, Feiyan; Lindberg, Pia
  • Scientific Reports, Vol. 6, Issue 1
  • DOI: 10.1038/srep36640

Rapid construction of metabolic models for a family of Cyanobacteria using a multiple source annotation workflow
journal, January 2013

  • Mueller, Thomas J.; Berla, Bertram M.; Pakrasi, Himadri B.
  • BMC Systems Biology, Vol. 7, Issue 1
  • DOI: 10.1186/1752-0509-7-142

Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria
journal, March 2017

  • Kanno, Masahiro; Carroll, Austin L.; Atsumi, Shota
  • Nature Communications, Vol. 8, Issue 1
  • DOI: 10.1038/ncomms14724

Genome Engineering in Cyanobacteria: Where We Are and Where We Need To Go
journal, June 2015

  • Ramey, C. Josh; Barón-Sola, Ángel; Aucoin, Hanna R.
  • ACS Synthetic Biology, Vol. 4, Issue 11
  • DOI: 10.1021/acssynbio.5b00043

Utilising the native plasmid, pCA2.4, from the cyanobacterium Synechocystis sp. strain PCC6803 as a cloning site for enhanced product production
journal, December 2015


Transformation of a filamentous cyanobacterium by electroporation.
journal, October 1989


Genome-scale modeling of Synechocystis sp. PCC 6803 and prediction of pathway insertion
journal, April 2009

  • Fu, Pengcheng
  • Journal of Chemical Technology & Biotechnology, Vol. 84, Issue 4
  • DOI: 10.1002/jctb.2065

A comprehensive genome‐scale reconstruction of Escherichia coli metabolism—2011
journal, January 2011

  • Orth, Jeffrey D.; Conrad, Tom M.; Na, Jessica
  • Molecular Systems Biology, Vol. 7, Issue 1
  • DOI: 10.1038/msb.2011.65

Engineering of genetic control tools in Synechocystis sp. PCC 6803 using rational design techniques
journal, December 2015


A conjugative plasmid vector for promoter analysis in several cyanobacteria of the genera Synechococcus and Synechocystis
journal, November 1993

  • Marraccini, Pierre; Bulteau, St�phane; Cassier-Chauvat, Corinne
  • Plant Molecular Biology, Vol. 23, Issue 4
  • DOI: 10.1007/BF00021546

May photoinhibition be a consequence, rather than a cause, of limited plant productivity?
journal, May 2013

  • Adams, William W.; Muller, Onno; Cohu, Christopher M.
  • Photosynthesis Research, Vol. 117, Issue 1-3
  • DOI: 10.1007/s11120-013-9849-7

Analysis of optimality in natural and perturbed metabolic networks
journal, November 2002

  • Segre, D.; Vitkup, D.; Church, G. M.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 23
  • DOI: 10.1073/pnas.232349399

The primary transcriptome of the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973
journal, August 2018


Synthetic Biology Guides Biofuel Production
journal, January 2010

  • Connor, Michael R.; Atsumi, Shota
  • Journal of Biomedicine and Biotechnology, Vol. 2010
  • DOI: 10.1155/2010/541698

Determining the optimal nitrogen source for large-scale cultivation of filamentous cyanobacteria
journal, August 2016

  • Johnson, Tylor J.; Jahandideh, Arash; Isaac, Isabel C.
  • Journal of Applied Phycology, Vol. 29, Issue 1
  • DOI: 10.1007/s10811-016-0923-3

Metabolic engineering with systems biology tools to optimize production of prokaryotic secondary metabolites
journal, January 2016

  • Kim, Hyun Uk; Charusanti, Pep; Lee, Sang Yup
  • Natural Product Reports, Vol. 33, Issue 8
  • DOI: 10.1039/C6NP00019C

Application of synthetic biology in cyanobacteria and algae
journal, September 2012

  • Wang, Bo; Wang, Jiangxin; Zhang, Weiwen
  • Frontiers in Microbiology, Vol. 3, Article No. 344
  • DOI: 10.3389/fmicb.2012.00344

Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication.
journal, January 1981


What is flux balance analysis?
journal, March 2010

  • Orth, Jeffrey D.; Thiele, Ines; Palsson, Bernhard Ø
  • Nature Biotechnology, Vol. 28, Issue 3
  • DOI: 10.1038/nbt.1614

Identifying the Metabolic Differences of a Fast-Growth Phenotype in Synechococcus UTEX 2973
journal, January 2017

  • Mueller, Thomas J.; Ungerer, Justin L.; Pakrasi, Himadri B.
  • Scientific Reports, Vol. 7, Issue 1
  • DOI: 10.1038/srep41569

Engineered CRISPR/Cas9 system for multiplex genome engineering of polyploid industrial yeast strains
journal, March 2018

  • Lian, Jiazhang; Bao, Zehua; Hu, Sumeng
  • Biotechnology and Bioengineering, Vol. 115, Issue 6
  • DOI: 10.1002/bit.26569

Constraining the metabolic genotype–phenotype relationship using a phylogeny of in silico methods
journal, February 2012

  • Lewis, Nathan E.; Nagarajan, Harish; Palsson, Bernhard O.
  • Nature Reviews Microbiology, Vol. 10, Issue 4
  • DOI: 10.1038/nrmicro2737

The Prospect of Using Cyanobacterial Bicarbonate Transporters to Improve Leaf Photosynthesis in C 3 Crop Plants
journal, October 2010

  • Price, G. Dean; Badger, Murray R.; von Caemmerer, Susanne
  • Plant Physiology, Vol. 155, Issue 1
  • DOI: 10.1104/pp.110.164681

Refining metabolic models and accounting for regulatory effects
journal, October 2014


Constructing kinetic models of metabolism at genome-scales: A review
journal, September 2015

  • Srinivasan, Shyam; Cluett, William R.; Mahadevan, Radhakrishnan
  • Biotechnology Journal, Vol. 10, Issue 9
  • DOI: 10.1002/biot.201400522

Cyanobacterial Farming for Environment Friendly Sustainable Agriculture Practices: Innovations and Perspectives
journal, February 2018


Nature-Driven Photochemistry for Catalytic Solar Hydrogen Production: A Photosystem I–Transition Metal Catalyst Hybrid
journal, October 2011

  • Utschig, Lisa M.; Silver, Sunshine C.; Mulfort, Karen L.
  • Journal of the American Chemical Society, Vol. 133, Issue 41
  • DOI: 10.1021/ja206012r

Fast automated reconstruction of genome-scale metabolic models for microbial species and communities
journal, June 2018

  • Machado, Daniel; Andrejev, Sergej; Tramontano, Melanie
  • Nucleic Acids Research, Vol. 46, Issue 15
  • DOI: 10.1093/nar/gky537

A simple method for isolation and construction of markerless cyanobacterial mutants defective in acyl-acyl carrier protein synthetase
journal, October 2016

  • Kojima, Kouji; Keta, Sumie; Uesaka, Kazuma
  • Applied Microbiology and Biotechnology, Vol. 100, Issue 23
  • DOI: 10.1007/s00253-016-7850-8

Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination.
journal, November 1994


The rrnA promoter as a tool for the improved expression of heterologous genes in cyanobacteria
journal, May 2014


Synthetic Biology: Advancing the Design of Diverse Genetic Systems
journal, June 2013


Transformation in Agmenellum quadruplicatum
journal, October 1980

  • Stevens, S. E.; Porter, R. D.
  • Proceedings of the National Academy of Sciences, Vol. 77, Issue 10
  • DOI: 10.1073/pnas.77.10.6052

Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria
journal, March 2014

  • Zhou, Jie; Zhang, Haifeng; Meng, Hengkai
  • Scientific Reports, Vol. 4, Issue 1
  • DOI: 10.1038/srep04500

An Organic Acid Based Counter Selection System for Cyanobacteria
journal, October 2013


The plasticity of cyanobacterial metabolism supports direct CO2 conversion to ethylene
journal, April 2015


The role of HetN in maintenance of the heterocyst pattern in Anabaena sp. PCC 7120
journal, May 2001


Salt stress is an environmental signal affecting degradative enzyme synthesis in Bacillus subtilis.
journal, May 1995


Comparative genomics reveals the molecular determinants of rapid growth of the cyanobacterium Synechococcus elongatus UTEX 2973
journal, November 2018

  • Ungerer, Justin; Wendt, Kristen E.; Hendry, John I.
  • Proceedings of the National Academy of Sciences, Vol. 115, Issue 50
  • DOI: 10.1073/pnas.1814912115

AraBAD Based Toolkit for Gene Expression and Metabolic Robustness Improvement in Synechococcus elongatus
journal, December 2017


Circadian Gating of the Cell Cycle Revealed in Single Cyanobacterial Cells
journal, March 2010


Genome-wide protein-protein interactions and protein function exploration in cyanobacteria
journal, October 2015

  • Lv, Qi; Ma, Weimin; Liu, Hui
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep15519

Evidence for genetic transformation in blue-green alga Anacystis nidulans
journal, January 1970

  • Shestakov, S. V.; Khyen, Nguyen Than
  • MGG Molecular & General Genetics, Vol. 107, Issue 4
  • DOI: 10.1007/BF00441199

The essential gene set of a photosynthetic organism
journal, October 2015

  • Rubin, Benjamin E.; Wetmore, Kelly M.; Price, Morgan N.
  • Proceedings of the National Academy of Sciences, Vol. 112, Issue 48
  • DOI: 10.1073/pnas.1519220112

Engineering Cyanobacterial Cell Morphology for Enhanced Recovery and Processing of Biomass
journal, February 2017

  • Jordan, Adam; Chandler, Jenna; MacCready, Joshua S.
  • Applied and Environmental Microbiology, Vol. 83, Issue 9
  • DOI: 10.1128/AEM.00053-17

Construction of shuttle vectors capable of conjugative transfer from Escherichia coli to nitrogen-fixing filamentous cyanobacteria.
journal, March 1984

  • Wolk, C. P.; Vonshak, A.; Kehoe, P.
  • Proceedings of the National Academy of Sciences, Vol. 81, Issue 5
  • DOI: 10.1073/pnas.81.5.1561

CRISPR interference as a titratable, trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002
journal, November 2016


In Silico Constraint-Based Strain Optimization Methods: the Quest for Optimal Cell Factories
journal, November 2015

  • Maia, Paulo; Rocha, Miguel; Rocha, Isabel
  • Microbiology and Molecular Biology Reviews, Vol. 80, Issue 1
  • DOI: 10.1128/MMBR.00014-15

Engineering and characterization of a superfolder green fluorescent protein
journal, December 2005

  • Pédelacq, Jean-Denis; Cabantous, Stéphanie; Tran, Timothy
  • Nature Biotechnology, Vol. 24, Issue 1, p. 79-88
  • DOI: 10.1038/nbt1172

A single vector-based strategy for marker-less gene replacement in Synechocystis sp. PCC 6803
journal, January 2014

  • Viola, Stefania; Rühle, Thilo; Leister, Dario
  • Microbial Cell Factories, Vol. 13, Issue 1
  • DOI: 10.1186/1475-2859-13-4

k-OptForce: Integrating Kinetics with Flux Balance Analysis for Strain Design
journal, February 2014


Reconstruction and Validation of a Genome-Scale Metabolic Model for the Filamentous Fungus Neurospora crassa Using FARM
journal, July 2013


A Cyanobacterial Circadian Timing Mechanism
journal, December 2003


Engineering the fatty acid synthesis pathway in Synechococcus elongatus PCC 7942 improves omega-3 fatty acid production
journal, September 2018

  • Santos-Merino, María; Garcillán-Barcia, M. Pilar; de la Cruz, Fernando
  • Biotechnology for Biofuels, Vol. 11, Issue 1
  • DOI: 10.1186/s13068-018-1243-4

Purification and properties of unicellular blue-green algae (order Chroococcales).
journal, January 1971


Using Genome-scale Models to Predict Biological Capabilities
journal, May 2015


Development of Synechocystis sp. PCC 6803 as a Phototrophic Cell Factory
journal, August 2013


Synthetic biology of cyanobacteria: unique challenges and opportunities
journal, January 2013


Crop Productivity and Photoassimilate Partitioning
journal, August 1984


Engineering photoautotrophic carbon fixation for enhanced growth and productivity
journal, January 2018

  • Liang, Feiyan; Lindberg, Pia; Lindblad, Peter
  • Sustainable Energy & Fuels, Vol. 2, Issue 12
  • DOI: 10.1039/C8SE00281A

The art of reporter proteins in science: past, present and future applications
journal, July 2010


Gene Transfer in Leptolyngbya sp. Strain BL0902, a Cyanobacterium Suitable for Production of Biomass and Bioproducts
journal, January 2012


System and method for research-scale outdoor production of microalgae and cyanobacteria
journal, August 2014


Methods for automated genome-scale metabolic model reconstruction
journal, July 2018

  • Faria, José P.; Rocha, Miguel; Rocha, Isabel
  • Biochemical Society Transactions, Vol. 46, Issue 4
  • DOI: 10.1042/BST20170246

BRENDA, enzyme data and metabolic information
journal, January 2002


Elucidation of photoautotrophic carbon flux topology in Synechocystis PCC 6803 using genome-scale carbon mapping models
journal, May 2018


OptORF: Optimal metabolic and regulatory perturbations for metabolic engineering of microbial strains
journal, January 2010

  • Kim, Joonhoon; Reed, Jennifer L.
  • BMC Systems Biology, Vol. 4, Issue 1, Article No. 53
  • DOI: 10.1186/1752-0509-4-53

A novel counter-selection method for markerless genetic modification in Synechocystis sp. PCC 6803
journal, December 2012

  • Cheah, Yi Ern; Albers, Stevan C.; Peebles, Christie A. M.
  • Biotechnology Progress, Vol. 29, Issue 1
  • DOI: 10.1002/btpr.1661

Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides
journal, July 2010

  • Warner, Joseph R.; Reeder, Philippa J.; Karimpour-Fard, Anis
  • Nature Biotechnology, Vol. 28, Issue 8, p. 856-862
  • DOI: 10.1038/nbt.1653

Systems metabolic engineering for chemicals and materials
journal, August 2011


Advances in Metabolic Engineering of Cyanobacteria for Photosynthetic Biochemical Production
journal, October 2015


A systems biology approach to reconcile metabolic network models with application to Synechocystis sp. PCC 6803 for biofuel production
journal, January 2016

  • Mohammadi, Reza; Fallah-Mehrabadi, Jalil; Bidkhori, Gholamreza
  • Molecular BioSystems, Vol. 12, Issue 8
  • DOI: 10.1039/C6MB00119J

Detailing the optimality of photosynthesis in cyanobacteria through systems biology analysis
journal, January 2012

  • Nogales, J.; Gudmundsson, S.; Knight, E. M.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 7
  • DOI: 10.1073/pnas.1117907109

Transformation in Cyanobacteria
journal, January 1986


High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
journal, June 2013

  • Fu, Yanfang; Foden, Jennifer A.; Khayter, Cyd
  • Nature Biotechnology, Vol. 31, Issue 9
  • DOI: 10.1038/nbt.2623

Biological targets and mechanisms of action of natural products from marine cyanobacteria
journal, January 2015

  • Salvador-Reyes, Lilibeth A.; Luesch, Hendrik
  • Natural Product Reports, Vol. 32, Issue 3
  • DOI: 10.1039/C4NP00104D

A Comprehensively Curated Genome-Scale Two-Cell Model for the Heterocystous Cyanobacterium Anabaena sp. PCC 7120
journal, November 2016

  • Malatinszky, David; Steuer, Ralf; Jones, Patrik R.
  • Plant Physiology, Vol. 173, Issue 1
  • DOI: 10.1104/pp.16.01487

Flux balance analysis of biological systems: applications and challenges
journal, March 2009

  • Raman, K.; Chandra, N.
  • Briefings in Bioinformatics, Vol. 10, Issue 4
  • DOI: 10.1093/bib/bbp011

MetRxn: a knowledgebase of metabolites and reactions spanning metabolic models and databases
journal, January 2012

  • Kumar, Akhil; Suthers, Patrick F.; Maranas, Costas D.
  • BMC Bioinformatics, Vol. 13, Issue 1
  • DOI: 10.1186/1471-2105-13-6

CyanoBase: a large-scale update on its 20th anniversary
journal, November 2016

  • Fujisawa, Takatomo; Narikawa, Rei; Maeda, Shin-ichi
  • Nucleic Acids Research, Vol. 45, Issue D1
  • DOI: 10.1093/nar/gkw1131

Ploidy in cyanobacteria
journal, September 2011


Site-directed mutagenesis in photosystem II of the cyanobacterium Synechocystis sp. PCC 6803: Donor D is a tyrosine residue in the D2 protein
journal, November 1988

  • Vermass, W. F. J.; Rutherford, A. W.; Hansson, O.
  • Proceedings of the National Academy of Sciences, Vol. 85, Issue 22
  • DOI: 10.1073/pnas.85.22.8477

Synthetic Biology and Metabolic Engineering
journal, November 2012

  • Stephanopoulos, Gregory
  • ACS Synthetic Biology, Vol. 1, Issue 11
  • DOI: 10.1021/sb300094q

Aquatic phototrophs: efficient alternatives to land-based crops for biofuels
journal, June 2008

  • Dismukes, G. Charles; Carrieri, Damian; Bennette, Nicholas
  • Current Opinion in Biotechnology, Vol. 19, Issue 3
  • DOI: 10.1016/j.copbio.2008.05.007

Computational evaluation of Synechococcus sp. PCC 7002 metabolism for chemical production
journal, April 2013

  • Vu, Trang T.; Hill, Eric A.; Kucek, Leo A.
  • Biotechnology Journal, Vol. 8, Issue 5
  • DOI: 10.1002/biot.201200315

Flux Balance Analysis of Photoautotrophic Metabolism
journal, December 2005

  • Shastri, A. A.; Morgan, J. A.
  • Biotechnology Progress, Vol. 21, Issue 6
  • DOI: 10.1021/bp050246d

The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production
journal, April 2016

  • Song, Kuo; Tan, Xiaoming; Liang, Yajing
  • Applied Microbiology and Biotechnology, Vol. 100, Issue 18
  • DOI: 10.1007/s00253-016-7510-z

Expanding the toolbox for Synechocystis sp. PCC 6803: validation of replicative vectors and characterization of a novel set of promoters
journal, January 2018

  • Ferreira, Eunice A.; Pacheco, Catarina C.; Pinto, Filipe
  • Synthetic Biology, Vol. 3, Issue 1
  • DOI: 10.1093/synbio/ysy014

Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology
journal, March 2010

  • Huang, Hsin-Ho; Camsund, Daniel; Lindblad, Peter
  • Nucleic Acids Research, Vol. 38, Issue 8
  • DOI: 10.1093/nar/gkq164

Systems analysis of ethanol production in the genetically engineered cyanobacterium Synechococcus sp. PCC 7002
journal, March 2017

  • Kopka, Joachim; Schmidt, Stefanie; Dethloff, Frederik
  • Biotechnology for Biofuels, Vol. 10, Issue 1
  • DOI: 10.1186/s13068-017-0741-0

Software platforms to facilitate reconstructing genome-scale metabolic networks: Software platforms for network reconstruction
journal, November 2013


Spatial and Temporal Organization of Chromosome Duplication and Segregation in the Cyanobacterium Synechococcus elongatus PCC 7942
journal, October 2012


Oxygen-responsive genetic circuits constructed in Synechocystis sp. PCC 6803 : Oxygen-Responsive Gene Circuits for
journal, September 2015

  • Immethun, Cheryl M.; Ng, Kenneth M.; DeLorenzo, Drew M.
  • Biotechnology and Bioengineering, Vol. 113, Issue 2
  • DOI: 10.1002/bit.25722

Metabolic engineering tools in model cyanobacteria
journal, November 2018


Recent advances in synthetic biology of cyanobacteria
journal, May 2018

  • Sengupta, Annesha; Pakrasi, Himadri B.; Wangikar, Pramod P.
  • Applied Microbiology and Biotechnology, Vol. 102, Issue 13
  • DOI: 10.1007/s00253-018-9046-x

Optknock: A bilevel programming framework for identifying gene knockout strategies for microbial strain optimization
journal, October 2003

  • Burgard, Anthony P.; Pharkya, Priti; Maranas, Costas D.
  • Biotechnology and Bioengineering, Vol. 84, Issue 6
  • DOI: 10.1002/bit.10803

Physical, chemical, and metabolic state sensors expand the synthetic biology toolbox for Synechocystis sp. PCC 6803 : New Sensors for
journal, March 2017

  • Immethun, Cheryl M.; DeLorenzo, Drew M.; Focht, Caroline M.
  • Biotechnology and Bioengineering, Vol. 114, Issue 7
  • DOI: 10.1002/bit.26275

The environmental photobioreactor (ePBR): An algal culturing platform for simulating dynamic natural environments
journal, October 2014


Toolboxes for cyanobacteria: Recent advances and future direction
journal, July 2018


Genome‐scale gene/reaction essentiality and synthetic lethality analysis
journal, January 2009

  • Suthers, Patrick F.; Zomorrodi, Alireza; Maranas, Costas D.
  • Molecular Systems Biology, Vol. 5, Issue 1
  • DOI: 10.1038/msb.2009.56

The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins
journal, February 2017

  • Rodriguez, Erik A.; Campbell, Robert E.; Lin, John Y.
  • Trends in Biochemical Sciences, Vol. 42, Issue 2
  • DOI: 10.1016/j.tibs.2016.09.010

Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system
journal, June 2014

  • Sakuma, Tetsushi; Nishikawa, Ayami; Kume, Satoshi
  • Scientific Reports, Vol. 4, Issue 1
  • DOI: 10.1038/srep05400

Deciphering cyanobacterial phenotypes for fast photoautotrophic growth via isotopically nonstationary metabolic flux analysis
journal, November 2017


Cyanobacterial defense mechanisms against foreign DNA transfer and their impact on genetic engineering
journal, January 2013


Metabolic engineering of Synechocystis sp. PCC 6803 for enhanced ethanol production based on flux balance analysis
journal, March 2017

  • Yoshikawa, Katsunori; Toya, Yoshihiro; Shimizu, Hiroshi
  • Bioprocess and Biosystems Engineering, Vol. 40, Issue 5
  • DOI: 10.1007/s00449-017-1744-8

Reconstruction of biochemical networks in microorganisms
journal, December 2008

  • Feist, Adam M.; Herrgård, Markus J.; Thiele, Ines
  • Nature Reviews Microbiology, Vol. 7, Issue 2
  • DOI: 10.1038/nrmicro1949

Increased Photochemical Efficiency in Cyanobacteria via an Engineered Sucrose Sink
journal, October 2016

  • Abramson, Bradley W.; Kachel, Benjamin; Kramer, David M.
  • Plant and Cell Physiology, Vol. 57, Issue 12
  • DOI: 10.1093/pcp/pcw169

OptForce: An Optimization Procedure for Identifying All Genetic Manipulations Leading to Targeted Overproductions
journal, April 2010

  • Ranganathan, Sridhar; Suthers, Patrick F.; Maranas, Costas D.
  • PLoS Computational Biology, Vol. 6, Issue 4
  • DOI: 10.1371/journal.pcbi.1000744

Improved Free Fatty Acid Production in Cyanobacteria with Synechococcus sp. PCC 7002 as Host
journal, May 2014


Evidence for the widespread distribution of CRISPR-Cas system in the Phylum Cyanobacteria
journal, February 2013

  • Cai, Fei; Axen, Seth D.; Kerfeld, Cheryl A.
  • RNA Biology, Vol. 10, Issue 5
  • DOI: 10.4161/rna.24571

Genetic Instability in Cyanobacteria – An Elephant in the Room?
journal, May 2014


Identification of a metabolic network structure representative ofarthrospira (spirulina) platensis metabolism
journal, October 2003

  • Cogne, Guillaume; Gros, J. -B.; Dussap, C. -G.
  • Biotechnology and Bioengineering, Vol. 84, Issue 6
  • DOI: 10.1002/bit.10808

TransportDB: a relational database of cellular membrane transport systems
journal, January 2004


A protocol for generating a high-quality genome-scale metabolic reconstruction
journal, January 2010


Physiological tolerance and stoichiometric potential of cyanobacteria for hydrocarbon fuel production
journal, November 2012


Regulation of Gene Expression in Diverse Cyanobacterial Species by Using Theophylline-Responsive Riboswitches
journal, August 2014

  • Ma, Amy T.; Schmidt, Calvin M.; Golden, James W.
  • Applied and Environmental Microbiology, Vol. 80, Issue 21
  • DOI: 10.1128/AEM.01697-14

SHARP: genome-scale identification of gene–protein–reaction associations in cyanobacteria
journal, August 2013

  • Krishnakumar, S.; Durai, Dilip A.; Wangikar, Pramod P.
  • Photosynthesis Research, Vol. 118, Issue 1-2
  • DOI: 10.1007/s11120-013-9910-6

Synthetic Biology: An Emerging Engineering Discipline
journal, August 2012


Comparative Genomics of DNA Recombination and Repair in Cyanobacteria: Biotechnological Implications
journal, November 2016

  • Cassier-Chauvat, Corinne; Veaudor, Théo; Chauvat, Franck
  • Frontiers in Microbiology, Vol. 7
  • DOI: 10.3389/fmicb.2016.01809

Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria
journal, July 2014

  • Taton, Arnaud; Unglaub, Federico; Wright, Nicole E.
  • Nucleic Acids Research, Vol. 42, Issue 17
  • DOI: 10.1093/nar/gku673

Cyanobacterial Heterocysts
journal, February 2010

  • Kumar, K.; Mella-Herrera, R. A.; Golden, J. W.
  • Cold Spring Harbor Perspectives in Biology, Vol. 2, Issue 4
  • DOI: 10.1101/cshperspect.a000315

Tapping the Unused Potential of Photosynthesis with a Heterologous Electron Sink
journal, July 2016

  • Berepiki, Adokiye; Hitchcock, Andrew; Moore, C. Mark
  • ACS Synthetic Biology, Vol. 5, Issue 12
  • DOI: 10.1021/acssynbio.6b00100

A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria
journal, March 2018

  • Kelly, Ciarán L.; Taylor, George M.; Hitchcock, Andrew
  • ACS Synthetic Biology, Vol. 7, Issue 4
  • DOI: 10.1021/acssynbio.7b00435

Metabolic Engineering of Bacteria
journal, March 2011


Evolution and diversity of CO2 concentrating mechanisms in cyanobacteria
journal, January 2002

  • Badger, Murray R.; Hanson, David; Price, G. Dean
  • Functional Plant Biology, Vol. 29, Issue 3
  • DOI: 10.1071/PP01213

Degradation of phenolic compounds by fresh-water algae
journal, March 1977


Expanding the Potential of CRISPR-Cpf1-Based Genome Editing Technology in the Cyanobacterium Anabaena PCC 7120
journal, December 2018


Identification and Regulation of Genes for Cobalamin Transport in the Cyanobacterium Synechococcus sp. Strain PCC 7002
journal, July 2016

  • Pérez, Adam A.; Rodionov, Dmitry A.; Bryant, Donald A.
  • Journal of Bacteriology, Vol. 198, Issue 19
  • DOI: 10.1128/JB.00476-16

Wide-dynamic-range promoters engineered for cyanobacteria
journal, January 2013

  • Huang, Hsin-Ho; Lindblad, Peter
  • Journal of Biological Engineering, Vol. 7, Issue 1
  • DOI: 10.1186/1754-1611-7-10

Engineered Transcriptional Systems for Cyanobacterial Biotechnology
journal, October 2014


Modelling cyanobacteria: from metabolism to integrative models of phototrophic growth
journal, March 2012

  • Steuer, R.; Knoop, H.; Machne, R.
  • Journal of Experimental Botany, Vol. 63, Issue 6
  • DOI: 10.1093/jxb/ers018

Constraints to commercialization of algal fuels
journal, September 2013


A genome-scale metabolic reconstruction of Pseudomonas putida KT2440: iJN746 as a cell factory
journal, January 2008

  • Nogales, Juan; Palsson, Bernhard Ø; Thiele, Ines
  • BMC Systems Biology, Vol. 2, Issue 1
  • DOI: 10.1186/1752-0509-2-79

Engineering BioBrick vectors from BioBrick parts
journal, January 2008

  • Shetty, Reshma P.; Endy, Drew; Knight, Thomas F.
  • Journal of Biological Engineering, Vol. 2, Article No. 5
  • DOI: 10.1186/1754-1611-2-5

CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973
journal, June 2016

  • Wendt, Kristen E.; Ungerer, Justin; Cobb, Ryan E.
  • Microbial Cell Factories, Vol. 15, Issue 1
  • DOI: 10.1186/s12934-016-0514-7

Applications of genome-scale metabolic network model in metabolic engineering
journal, December 2014

  • Kim, Byoungjin; Kim, Won Jun; Kim, Dong In
  • Journal of Industrial Microbiology & Biotechnology, Vol. 42, Issue 3
  • DOI: 10.1007/s10295-014-1554-9

Effects of temperature shifts on growth rate and lipid characteristics of Synechocystis sp. PCC6803 in a bench-top photobioreactor
journal, December 2011


Genome-Scale Modeling of Light-Driven Reductant Partitioning and Carbon Fluxes in Diazotrophic Unicellular Cyanobacterium Cyanothece sp. ATCC 51142
journal, April 2012


NOT Gate Genetic Circuits to Control Gene Expression in Cyanobacteria
journal, August 2017


The Metabolic Network of Synechocystis sp. PCC 6803: Systemic Properties of Autotrophic Growth
journal, July 2010

  • Knoop, Henning; Zilliges, Yvonne; Lockau, Wolfgang
  • Plant Physiology, Vol. 154, Issue 1
  • DOI: 10.1104/pp.110.157198

Evaluation of central metabolism based on a genomic database ofSynechocystis PCC6803
journal, April 2007

  • Hong, Seong-Joo; Lee, Choul-Gyun
  • Biotechnology and Bioprocess Engineering, Vol. 12, Issue 2
  • DOI: 10.1007/BF03028644

Hydrogenases and Hydrogen Metabolism of Cyanobacteria
journal, March 2002

  • Tamagnini, P.; Axelsson, R.; Lindberg, P.
  • Microbiology and Molecular Biology Reviews, Vol. 66, Issue 1
  • DOI: 10.1128/MMBR.66.1.1-20.2002

Circadian Control of Global Gene Expression by the Cyanobacterial Master Regulator RpaA
journal, December 2013


Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO2
journal, January 2015

  • Yu, Jingjie; Liberton, Michelle; Cliften, Paul F.
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep08132

    Works referencing / citing this record:

    A Novel Cyanobacterium Synechococcus elongatus PCC 11802 has Distinct Genomic and Metabolomic Characteristics Compared to its Neighbor PCC 11801
    journal, January 2020


    Bioengineering tools for the production of pharmaceuticals: current perspective and future outlook
    journal, January 2019


    Comprehensive Analyses of Cytochrome P450 Monooxygenases and Secondary Metabolite Biosynthetic Gene Clusters in Cyanobacteria
    journal, January 2020

    • Khumalo, Makhosazana Jabulile; Nzuza, Nomfundo; Padayachee, Tiara
    • International Journal of Molecular Sciences, Vol. 21, Issue 2
    • DOI: 10.3390/ijms21020656