The Green Berry Consortia of the Sippewissett Salt Marsh: Millimeter-Sized Aggregates of Diazotrophic Unicellular Cyanobacteria
Abstract
Microbial interactions driving key biogeochemical fluxes often occur within multispecies consortia that form spatially heterogeneous microenvironments. Here, we describe the “green berry” consortia of the Sippewissett salt marsh (Falmouth, MA, United States): millimeter-sized aggregates dominated by an uncultured, diazotrophic unicellular cyanobacterium of the order Chroococcales (termed GB-CYN1). We show that GB-CYN1 is closely related to Crocosphaera watsonii (UCYN-B) and “Candidatus Atelocyanobacterium thalassa” (UCYN-A), two groups of unicellular diazotrophic cyanobacteria that play an important role in marine primary production. Other green berry consortium members include pennate diatoms and putative heterotrophic bacteria from the Alphaproteobacteria and Bacteroidetes. Tight coupling was observed between photosynthetic oxygen production and heterotrophic respiration. When illuminated, the green berries became supersaturated with oxygen. From the metagenome, we observed that GB-CYN1 encodes photosystem II genes and thus has the metabolic potential for oxygen production unlike UCYN-A. In darkness, respiratory activity rapidly depleted oxygen creating anoxia within the aggregates. Metagenomic data revealed a suite of nitrogen fixation genes encoded by GB-CYN1, and nitrogenase activity was confirmed at the whole-aggregate level by acetylene reduction assays. Metagenome reads homologous to marker genes for denitrification were observed and suggest that heterotrophic denitrifiers might co-occur in the green berries, although the physiology and activitymore »
- Authors:
-
- Univ. of California, Santa Barbara, CA (United States). Dept. of Ecology, Evolution, and Marine Biology
- Max Planck Inst. for Marine Microbiology, Bremen (Germany). HGF MPG Joint Research Group for Deep Sea Ecology and Tech.
- Max Planck Inst. for Marine Microbiology, Bremen (Germany). Dept. for Microbiology; Council of Norway, Oslo (Norway). Div. for Science, Research
- Harvard Univ., Cambridge, MA (United States). Dept. of Organismic and Evolutionary Biology
- Univ. of California, Davis, CA (United States). Genome Center, Dept. of Evolution and Ecology
- Cornell Univ., Ithaca, NY (United States). School of Integrative Plant Science
- Cornell Univ., Ithaca, NY (United States). Dept. of Microbiology
- Publication Date:
- Research Org.:
- Marine Biological Lab., Woods Hole, MA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1468986
- Grant/Contract Number:
- FG02-85ER13361
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Microbiology
- Additional Journal Information:
- Journal Volume: 8; Journal ID: ISSN 1664-302X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Wilbanks, Elizabeth G., Salman-Carvalho, Verena, Jaekel, Ulrike, Humphrey, Parris T., Eisen, Jonathan A., Buckley, Daniel H., and Zinder, Stephen H. The Green Berry Consortia of the Sippewissett Salt Marsh: Millimeter-Sized Aggregates of Diazotrophic Unicellular Cyanobacteria. United States: N. p., 2017.
Web. doi:10.3389/fmicb.2017.01623.
Wilbanks, Elizabeth G., Salman-Carvalho, Verena, Jaekel, Ulrike, Humphrey, Parris T., Eisen, Jonathan A., Buckley, Daniel H., & Zinder, Stephen H. The Green Berry Consortia of the Sippewissett Salt Marsh: Millimeter-Sized Aggregates of Diazotrophic Unicellular Cyanobacteria. United States. https://doi.org/10.3389/fmicb.2017.01623
Wilbanks, Elizabeth G., Salman-Carvalho, Verena, Jaekel, Ulrike, Humphrey, Parris T., Eisen, Jonathan A., Buckley, Daniel H., and Zinder, Stephen H. Mon .
"The Green Berry Consortia of the Sippewissett Salt Marsh: Millimeter-Sized Aggregates of Diazotrophic Unicellular Cyanobacteria". United States. https://doi.org/10.3389/fmicb.2017.01623. https://www.osti.gov/servlets/purl/1468986.
@article{osti_1468986,
title = {The Green Berry Consortia of the Sippewissett Salt Marsh: Millimeter-Sized Aggregates of Diazotrophic Unicellular Cyanobacteria},
author = {Wilbanks, Elizabeth G. and Salman-Carvalho, Verena and Jaekel, Ulrike and Humphrey, Parris T. and Eisen, Jonathan A. and Buckley, Daniel H. and Zinder, Stephen H.},
abstractNote = {Microbial interactions driving key biogeochemical fluxes often occur within multispecies consortia that form spatially heterogeneous microenvironments. Here, we describe the “green berry” consortia of the Sippewissett salt marsh (Falmouth, MA, United States): millimeter-sized aggregates dominated by an uncultured, diazotrophic unicellular cyanobacterium of the order Chroococcales (termed GB-CYN1). We show that GB-CYN1 is closely related to Crocosphaera watsonii (UCYN-B) and “Candidatus Atelocyanobacterium thalassa” (UCYN-A), two groups of unicellular diazotrophic cyanobacteria that play an important role in marine primary production. Other green berry consortium members include pennate diatoms and putative heterotrophic bacteria from the Alphaproteobacteria and Bacteroidetes. Tight coupling was observed between photosynthetic oxygen production and heterotrophic respiration. When illuminated, the green berries became supersaturated with oxygen. From the metagenome, we observed that GB-CYN1 encodes photosystem II genes and thus has the metabolic potential for oxygen production unlike UCYN-A. In darkness, respiratory activity rapidly depleted oxygen creating anoxia within the aggregates. Metagenomic data revealed a suite of nitrogen fixation genes encoded by GB-CYN1, and nitrogenase activity was confirmed at the whole-aggregate level by acetylene reduction assays. Metagenome reads homologous to marker genes for denitrification were observed and suggest that heterotrophic denitrifiers might co-occur in the green berries, although the physiology and activity of facultative anaerobes in these aggregates remains uncharacterized. Nitrogen fixation in the surface ocean was long thought to be driven by filamentous cyanobacterial aggregates, though recent work has demonstrated the importance of unicellular diazotrophic cyanobacteria (UCYN) from the order Chroococcales. Here, the green berries serve as a useful contrast to studies of open ocean UCYN and may provide a tractable model system to investigate microbial dynamics within phytoplankton aggregates, a phenomenon of global importance to the flux of particulate organic carbon and nitrogen in surface waters.},
doi = {10.3389/fmicb.2017.01623},
journal = {Frontiers in Microbiology},
number = ,
volume = 8,
place = {United States},
year = {Mon Sep 04 00:00:00 EDT 2017},
month = {Mon Sep 04 00:00:00 EDT 2017}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 3 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Evidence and a conceptual model for the co‑occurrence of nitrogen fixation and denitrification in heterotrophic marine sediments
journal, May 2013
- Fulweiler, Rw; Brown, Sm; Nixon, Sw
- Marine Ecology Progress Series, Vol. 482
Direct measurement of diffusivity within diatom aggregates containing transparent exopolymer particles
journal, January 2007
- Ploug, Helle; Passow, Uta
- Limnology and Oceanography, Vol. 52, Issue 1
Experiments linking nitrogenase gene expression to nitrogen fixation in the North Pacific subtropical gyre
journal, January 2007
- Zehr, Jonathan P.; Montoya, Joseph P.; Jenkins, Bethany D.
- Limnology and Oceanography, Vol. 52, Issue 1
New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0
journal, March 2010
- Guindon, Stéphane; Dufayard, Jean-François; Lefort, Vincent
- Systematic Biology, Vol. 59, Issue 3
Composition, uniqueness and variability of the epiphytic bacterial community of the green alga Ulva australis
journal, November 2010
- Burke, Catherine; Thomas, Torsten; Lewis, Matt
- The ISME Journal, Vol. 5, Issue 4
Phenotypic and genotypic characterization of multiple strains of the diazotrophic cyanobacterium, Crocosphaera watsonii , isolated from the open ocean
journal, February 2009
- Webb, Eric A.; Ehrenreich, Ian M.; Brown, Susan L.
- Environmental Microbiology, Vol. 11, Issue 2
Baltic Sea cyanobacterial bloom contains denitrification and nitrification genes, but has negligible denitrification activity
journal, July 2003
- Tuomainen, Jaana M.; Hietanen, Susanna; Kuparinen, Jorma
- FEMS Microbiology Ecology, Vol. 45, Issue 2
Community Structure and Functional Gene Profile of Bacteria on Healthy and Diseased Thalli of the Red Seaweed Delisea pulchra
journal, December 2012
- Fernandes, Neil; Steinberg, Peter; Rusch, Doug
- PLoS ONE, Vol. 7, Issue 12
Globally Distributed Uncultivated Oceanic N2-Fixing Cyanobacteria Lack Oxygenic Photosystem II
journal, November 2008
- Zehr, J. P.; Bench, S. R.; Carter, B. J.
- Science, Vol. 322, Issue 5904
Spatial coupling of nitrogen inputs and losses in the ocean
journal, January 2007
- Deutsch, Curtis; Sarmiento, Jorge L.; Sigman, Daniel M.
- Nature, Vol. 445, Issue 7124
Metabolic streamlining in an open-ocean nitrogen-fixing cyanobacterium
journal, February 2010
- Tripp, H. James; Bench, Shellie R.; Turk, Kendra A.
- Nature, Vol. 464, Issue 7285
Microbial community gene expression within colonies of the diazotroph, Trichodesmium, from the Southwest Pacific Ocean
journal, July 2009
- Hewson, Ian; Poretsky, Rachel S.; Dyhrman, Sonya T.
- The ISME Journal, Vol. 3, Issue 11
Bacterial Associations with Marine Oscillatoria sp. (Trichodesmium sp.) Populations: Ecophysiological Implications1
journal, December 1989
- Paerly, Hans W.; Bebout, Brad M.; Prufert, Leslie E.
- Journal of Phycology, Vol. 25, Issue 4
Novel Metabolic Attributes of the Genus Cyanothece, Comprising a Group of Unicellular Nitrogen-Fixing Cyanobacteria
journal, October 2011
- Bandyopadhyay, Anindita; Elvitigala, Thanura; Welsh, Eric
- mBio, Vol. 2, Issue 5
Influence of the Amazon River plume on distributions of free-living and symbiotic cyanobacteria in the western tropical north Atlantic Ocean
journal, March 2007
- Foster, R. A.; Subramaniam, A.; Mahaffey, C.
- Limnology and Oceanography, Vol. 52, Issue 2
No sign of denitrification in a Baltic Sea cyanobacterial bloom
journal, January 2002
- Hietanen, S.; Moisander, Ph; Kuparinen, J.
- Marine Ecology Progress Series, Vol. 242
FastTree 2 – Approximately Maximum-Likelihood Trees for Large Alignments
journal, March 2010
- Price, Morgan N.; Dehal, Paramvir S.; Arkin, Adam P.
- PLoS ONE, Vol. 5, Issue 3
Compositional differences in particle-associated and free-living microbial assemblages from an extreme deep-ocean environment: Microbial diversity in the Puerto Rico Trench
journal, November 2010
- Eloe, Emiley A.; Shulse, Christine N.; Fadrosh, Douglas W.
- Environmental Microbiology Reports, Vol. 3, Issue 4
Aerobic and anaerobic nitrogen transformation processes in N2-fixing cyanobacterial aggregates
journal, January 2015
- Klawonn, Isabell; Bonaglia, Stefano; Brüchert, Volker
- The ISME Journal, Vol. 9, Issue 6
RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models
journal, August 2006
- Stamatakis, Alexandros
- Bioinformatics, Vol. 22, Issue 21
Nitrogen Cycles: Past, Present, and Future
journal, September 2004
- Galloway, J. N.; Dentener, F. J.; Capone, D. G.
- Biogeochemistry, Vol. 70, Issue 2
Constitutive Extracellular Polysaccharide (EPS) Production by Specific Isolates of Crocosphaera watsonii
journal, January 2011
- Sohm, Jill A.; Edwards, Bethanie R.; Wilson, Brian G.
- Frontiers in Microbiology, Vol. 2
Photoperiod length paces the temporal orchestration of cell cycle and carbon-nitrogen metabolism in Crocosphaera watsonii : Impact of PPL on C-N metabolism and cell cycle
journal, July 2013
- Dron, Anthony; Rabouille, Sophie; Claquin, Pascal
- Environmental Microbiology, Vol. 15, Issue 12
The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle
journal, September 2008
- Welsh, E. A.; Liberton, M.; Stockel, J.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 39
The nitrogen-fixing gene (nifH) of Rhodopseudomonas palustris: a case of lateral gene transfer?
journal, July 2004
- Cantera, J. J. L.
- Microbiology, Vol. 150, Issue 7
Characterization of diatom–cyanobacteria symbioses on the basis of nifH, hetR and 16S rRNA sequences
journal, November 2006
- Foster, Rachel A.; Zehr, Jonathan P.
- Environmental Microbiology, Vol. 8, Issue 11
Carbon, nitrogen and O2 fluxes associated with the cyanobacterium Nodularia spumigena in the Baltic Sea
journal, March 2011
- Ploug, Helle; Adam, Birgit; Musat, Niculina
- The ISME Journal, Vol. 5, Issue 9
Carbon and nitrogen fluxes associated with the cyanobacterium Aphanizomenon sp. in the Baltic Sea
journal, April 2010
- Ploug, Helle; Musat, Niculina; Adam, Birgit
- The ISME Journal, Vol. 4, Issue 9
Unicellular, aerobic nitrogen-fixing cyanobacteria of the genus Cyanothece.
journal, March 1993
- Reddy, K. J.; Haskell, J. B.; Sherman, D. M.
- Journal of Bacteriology, Vol. 175, Issue 5
Multicellular photo-magnetotactic bacteria: Multicellular photo-magnetotactic bacteria
journal, September 2010
- Shapiro, Orr H.; Hatzenpichler, Roland; Buckley, Daniel H.
- Environmental Microbiology Reports, Vol. 3, Issue 2
Two Strains of Crocosphaera watsonii with Highly Conserved Genomes are Distinguished by Strain-Specific Features
journal, January 2011
- Bench, Shellie R.; Ilikchyan, Irina N.; Tripp, H. James
- Frontiers in Microbiology, Vol. 2
SINA: Accurate high-throughput multiple sequence alignment of ribosomal RNA genes
journal, May 2012
- Pruesse, Elmar; Peplies, Jörg; Glöckner, Frank Oliver
- Bioinformatics, Vol. 28, Issue 14
The SILVA ribosomal RNA gene database project: improved data processing and web-based tools
journal, November 2012
- Quast, Christian; Pruesse, Elmar; Yilmaz, Pelin
- Nucleic Acids Research, Vol. 41, Issue D1
ProtTest 3: fast selection of best-fit models of protein evolution
journal, February 2011
- Darriba, Diego; Taboada, Guillermo L.; Doallo, Ramón
- Bioinformatics, Vol. 27, Issue 8
Systematic Identification of Gene Families for Use as “Markers” for Phylogenetic and Phylogeny-Driven Ecological Studies of Bacteria and Archaea and Their Major Subgroups
journal, October 2013
- Wu, Dongying; Jospin, Guillaume; Eisen, Jonathan A.
- PLoS ONE, Vol. 8, Issue 10
ARB: a software environment for sequence data
journal, February 2004
- Ludwig, W.
- Nucleic Acids Research, Vol. 32, Issue 4
Physiology of purple sulfur bacteria forming macroscopic aggregates in Great Sippewissett Salt Marsh, Massachusetts
journal, August 1993
- Seitz, Angelica P.; Nielsen, Tommy H.; Overmann, Jörg
- FEMS Microbiology Ecology, Vol. 12, Issue 4
Accelerated Profile HMM Searches
journal, October 2011
- Eddy, Sean R.
- PLoS Computational Biology, Vol. 7, Issue 10
Whole genome comparison of six Crocosphaera watsonii strains with differing phenotypes
journal, July 2013
- Bench, Shellie R.; Heller, Philip; Frank, Ildiko
- Journal of Phycology, Vol. 49, Issue 4
Ecological Patterns of nifH Genes in Four Terrestrial Climatic Zones Explored with Targeted Metagenomics Using FrameBot, a New Informatics Tool
journal, September 2013
- Wang, Qiong; Quensen, John F.; Fish, Jordan A.
- mBio, Vol. 4, Issue 5
Strain-level genomic variation in natural populations of Lebetimonas from an erupting deep-sea volcano
journal, November 2013
- Meyer, Julie L.; Huber, Julie A.
- The ISME Journal, Vol. 8, Issue 4
Photosynthetic characteristics of Trichodesmium in the southwest Pacific Ocean: importance and significance
journal, May 2007
- Masotti, I.; Ruiz-Pino, D.; Le Bouteiller, A.
- Marine Ecology Progress Series, Vol. 338
Basic local alignment search tool
journal, October 1990
- Altschul, Stephen F.; Gish, Warren; Miller, Webb
- Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
Legumes select symbiosis island sequence variants in Bradyrhizobium : SYMBIOSIS ISLAND VARIANTS IN
journal, February 2012
- Parker, Matthew A.
- Molecular Ecology, Vol. 21, Issue 7
Complexity of cyanobacterial exopolysaccharides: composition, structures, inducing factors and putative genes involved in their biosynthesis and assembly
journal, September 2009
- Pereira, Sara; Zille, Andrea; Micheletti, Ernesto
- FEMS Microbiology Reviews, Vol. 33, Issue 5
Unicellular Cyanobacterium Symbiotic with a Single-Celled Eukaryotic Alga
journal, September 2012
- Thompson, A. W.; Foster, R. A.; Krupke, A.
- Science, Vol. 337, Issue 6101
Light-dark (12:12) cycle of carbon and nitrogen metabolism in Crocosphaera watsonii WH8501: relation to the cell cycle: Daily C and N metabolism in C. watsonii WH8501
journal, December 2011
- Dron, Anthony; Rabouille, Sophie; Claquin, Pascal
- Environmental Microbiology, Vol. 14, Issue 4
Direct Measurement of O2-Depleted Microzones in Marine Oscillatoria: Relation to N2 Fixation
journal, July 1988
- Paerl, H. W.; Bebout, B. M.
- Science, Vol. 241, Issue 4864
MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space
journal, February 2012
- Ronquist, Fredrik; Teslenko, Maxim; van der Mark, Paul
- Systematic Biology, Vol. 61, Issue 3
Production and characterization of extracellular carbohydrate polymer from Cyanothece sp. CCY 0110
journal, February 2013
- Mota, Rita; Guimarães, Rodrigo; Büttel, Zsófia
- Carbohydrate Polymers, Vol. 92, Issue 2
Cyanobacterial surface blooms formed by Aphanizomenon sp. and Nodularia spumigena in the Baltic Sea: Small-scale fluxes, pH, and oxygen microenvironments
journal, May 2008
- Ploug, Helle
- Limnology and Oceanography, Vol. 53, Issue 3
High rates of N2 fixation by unicellular diazotrophs in the oligotrophic Pacific Ocean
journal, August 2004
- Montoya, Joseph P.; Holl, Carolyn M.; Zehr, Jonathan P.
- Nature, Vol. 430, Issue 7003
Genome sequencing in microfabricated high-density picolitre reactors
journal, July 2005
- Margulies, Marcel; Egholm, Michael; Altman, William E.
- Nature, Vol. 437, Issue 7057, p. 376-380
Trichodesmium, a Globally Significant Marine Cyanobacterium
journal, May 1997
- Capone, D. G.
- Science, Vol. 276, Issue 5316
Nitrogenase gene diversity and microbial community structure: a cross-system comparison
journal, July 2003
- Zehr, Jonathan P.; Jenkins, Bethany D.; Short, Steven M.
- Environmental Microbiology, Vol. 5, Issue 7
Community Structure of the Bacteria Associated with Nodularia sp. (Cyanobacteria) Aggregates in the Baltic Sea
journal, August 2006
- Tuomainen, Jaana; Hietanen, Susanna; Kuparinen, Jorma
- Microbial Ecology, Vol. 52, Issue 3
Horizontal transfer of the nitrogen fixation gene cluster in the cyanobacterium Microcoleus chthonoplastes
journal, September 2009
- Bolhuis, Henk; Severin, Ina; Confurius-Guns, Veronique
- The ISME Journal, Vol. 4, Issue 1
Primary production of microalgae in sediments measured by oxygen microprofile, H 14 CO 3 - fixation, and oxygen exchange methods1 : Primary production of sediments
journal, July 1981
- Revsbech, Niels Peter; JØrgensen, Bo Barker; Brix, Ole
- Limnology and Oceanography, Vol. 26, Issue 4
Nitrogen fixation in freshwater, estuarine, and marine ecosystems. 1. Rates and importance
journal, January 1988
- Howarth, Robert W.; Marino, Roxanne; Lane, Judith
- Limnology and Oceanography, Vol. 33, Issue 4_part_2
The metagenomics RAST server – a public resource for the automatic phylogenetic and functional analysis of metagenomes
journal, September 2008
- Meyer, F.; Paarmann, D.; D'Souza, M.
- BMC Bioinformatics, Vol. 9, Issue 1
Discovery of an Endosymbiotic Nitrogen-Fixing Cyanobacterium UCYN-A in Braarudosphaera bigelowii (Prymnesiophyceae)
journal, December 2013
- Hagino, Kyoko; Onuma, Ryo; Kawachi, Masanobu
- PLoS ONE, Vol. 8, Issue 12
Diffusion characteristics of microbial communities determined by use of oxygen microsensors
journal, February 1989
- Revsbech, Niels Peter
- Journal of Microbiological Methods, Vol. 9, Issue 2
Anoxic aggregates - an ephemeral phenomenon in the pelagic environment?
journal, January 1997
- Ploug, H.; Kühl, M.; Buchholz-Cleven, B.
- Aquatic Microbial Ecology, Vol. 13
Microscale sulfur cycling in the phototrophic pink berry consortia of the Sippewissett Salt Marsh: Sulfur cycling in pink berries of the Sippewissett Marsh
journal, February 2014
- Wilbanks, Elizabeth G.; Jaekel, Ulrike; Salman, Verena
- Environmental Microbiology, Vol. 16, Issue 11
Nitrogen fixation by algae in a Massachusetts salt marsh 1: N
journal, March 1978
- Carpenter, Edward J.; Van Raalte, Charlene D.; Valiela, Ivan
- Limnology and Oceanography, Vol. 23, Issue 2
Phylogenetic diversity of aggregate-attached vs. free-living marine bacterial assemblages
journal, July 1993
- DeLong, Edward F.; Franks, Diana G.; Alldredge, Alice L.
- Limnology and Oceanography, Vol. 38, Issue 5
A comprehensive aligned nifH gene database: a multipurpose tool for studies of nitrogen-fixing bacteria
journal, January 2014
- Gaby, John Christian; Buckley, Daniel H.
- Database, Vol. 2014
Community Structure of the Bacteria Associated with Nodularia sp. (Cyanobacteria) Aggregates in the Baltic Sea
journal, August 2006
- Tuomainen, Jaana; Hietanen, Susanna; Kuparinen, Jorma
- Microbial Ecology, Vol. 52, Issue 3
Nitrogen Cycles: Past, Present, and Future
journal, September 2004
- Galloway, J. N.; Dentener, F. J.; Capone, D. G.
- Biogeochemistry, Vol. 70, Issue 2
Diffusion characteristics of microbial communities determined by use of oxygen microsensors
journal, February 1989
- Revsbech, Niels Peter
- Journal of Microbiological Methods, Vol. 9, Issue 2
Production and characterization of extracellular carbohydrate polymer from Cyanothece sp. CCY 0110
journal, February 2013
- Mota, Rita; Guimarães, Rodrigo; Büttel, Zsófia
- Carbohydrate Polymers, Vol. 92, Issue 2
Transparent exopolymer particles (TEP) in aquatic environments
journal, November 2002
- Passow, U.
- Progress in Oceanography, Vol. 55, Issue 3-4
Microbial community gene expression within colonies of the diazotroph, Trichodesmium, from the Southwest Pacific Ocean
journal, July 2009
- Hewson, Ian; Poretsky, Rachel S.; Dyhrman, Sonya T.
- The ISME Journal, Vol. 3, Issue 11
Horizontal transfer of the nitrogen fixation gene cluster in the cyanobacterium Microcoleus chthonoplastes
journal, September 2009
- Bolhuis, Henk; Severin, Ina; Confurius-Guns, Veronique
- The ISME Journal, Vol. 4, Issue 1
Composition, uniqueness and variability of the epiphytic bacterial community of the green alga Ulva australis
journal, November 2010
- Burke, Catherine; Thomas, Torsten; Lewis, Matt
- The ISME Journal, Vol. 5, Issue 4
Carbon, nitrogen and O2 fluxes associated with the cyanobacterium Nodularia spumigena in the Baltic Sea
journal, March 2011
- Ploug, Helle; Adam, Birgit; Musat, Niculina
- The ISME Journal, Vol. 5, Issue 9
Strain-level genomic variation in natural populations of Lebetimonas from an erupting deep-sea volcano
journal, November 2013
- Meyer, Julie L.; Huber, Julie A.
- The ISME Journal, Vol. 8, Issue 4
Aerobic and anaerobic nitrogen transformation processes in N2-fixing cyanobacterial aggregates
journal, January 2015
- Klawonn, Isabell; Bonaglia, Stefano; Brüchert, Volker
- The ISME Journal, Vol. 9, Issue 6
High rates of N2 fixation by unicellular diazotrophs in the oligotrophic Pacific Ocean
journal, August 2004
- Montoya, Joseph P.; Holl, Carolyn M.; Zehr, Jonathan P.
- Nature, Vol. 430, Issue 7003
Genome sequencing in microfabricated high-density picolitre reactors
journal, July 2005
- Margulies, Marcel; Egholm, Michael; Altman, William E.
- Nature, Vol. 437, Issue 7057, p. 376-380
Spatial coupling of nitrogen inputs and losses in the ocean
journal, January 2007
- Deutsch, Curtis; Sarmiento, Jorge L.; Sigman, Daniel M.
- Nature, Vol. 445, Issue 7124
Metabolic streamlining in an open-ocean nitrogen-fixing cyanobacterium
journal, February 2010
- Tripp, H. James; Bench, Shellie R.; Turk, Kendra A.
- Nature, Vol. 464, Issue 7285
Genetic basis for the establishment of endosymbiosis in Paramecium
journal, January 2019
- He, Ming; Wang, Jinfeng; Fan, Xinpeng
- The ISME Journal, Vol. 13, Issue 5
Nitrogenase gene diversity and microbial community structure: a cross-system comparison
journal, July 2003
- Zehr, Jonathan P.; Jenkins, Bethany D.; Short, Steven M.
- Environmental Microbiology, Vol. 5, Issue 7
The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle
journal, September 2008
- Welsh, E. A.; Liberton, M.; Stockel, J.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 39
ProtTest 3: fast selection of best-fit models of protein evolution
journal, February 2011
- Darriba, Diego; Taboada, Guillermo L.; Doallo, Ramón
- Bioinformatics, Vol. 27, Issue 8
SINA: Accurate high-throughput multiple sequence alignment of ribosomal RNA genes
journal, May 2012
- Pruesse, Elmar; Peplies, Jörg; Glöckner, Frank Oliver
- Bioinformatics, Vol. 28, Issue 14
A comprehensive aligned nifH gene database: a multipurpose tool for studies of nitrogen-fixing bacteria
journal, January 2014
- Gaby, John Christian; Buckley, Daniel H.
- Database, Vol. 2014
The SILVA ribosomal RNA gene database project: improved data processing and web-based tools
journal, November 2012
- Quast, Christian; Pruesse, Elmar; Yilmaz, Pelin
- Nucleic Acids Research, Vol. 41, Issue D1
New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0
journal, March 2010
- Guindon, Stéphane; Dufayard, Jean-François; Lefort, Vincent
- Systematic Biology, Vol. 59, Issue 3
MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space
journal, February 2012
- Ronquist, Fredrik; Teslenko, Maxim; van der Mark, Paul
- Systematic Biology, Vol. 61, Issue 3
The nitrogen-fixing gene (nifH) of Rhodopseudomonas palustris: a case of lateral gene transfer?
journal, July 2004
- Cantera, J. J. L.
- Microbiology, Vol. 150, Issue 7
Bacterial Associations with Marine Oscillatoria sp. (Trichodesmium sp.) Populations: Ecophysiological Implications1
journal, December 1989
- Paerly, Hans W.; Bebout, Brad M.; Prufert, Leslie E.
- Journal of Phycology, Vol. 25, Issue 4
Characterization of diatom–cyanobacteria symbioses on the basis of nifH, hetR and 16S rRNA sequences
journal, November 2006
- Foster, Rachel A.; Zehr, Jonathan P.
- Environmental Microbiology, Vol. 8, Issue 11
Phenotypic and genotypic characterization of multiple strains of the diazotrophic cyanobacterium, Crocosphaera watsonii , isolated from the open ocean
journal, February 2009
- Webb, Eric A.; Ehrenreich, Ian M.; Brown, Susan L.
- Environmental Microbiology, Vol. 11, Issue 2
Physiology of purple sulfur bacteria forming macroscopic aggregates in Great Sippewissett Salt Marsh, Massachusetts
journal, August 1993
- Seitz, Angelica P.; Nielsen, Tommy H.; Overmann, Jörg
- FEMS Microbiology Ecology, Vol. 12, Issue 4
Complexity of cyanobacterial exopolysaccharides: composition, structures, inducing factors and putative genes involved in their biosynthesis and assembly
journal, September 2009
- Pereira, Sara; Zille, Andrea; Micheletti, Ernesto
- FEMS Microbiology Reviews, Vol. 33, Issue 5
Whole genome comparison of six Crocosphaera watsonii strains with differing phenotypes
journal, July 2013
- Bench, Shellie R.; Heller, Philip; Frank, Ildiko
- Journal of Phycology, Vol. 49, Issue 4
Globally Distributed Uncultivated Oceanic N2-Fixing Cyanobacteria Lack Oxygenic Photosystem II
journal, November 2008
- Zehr, J. P.; Bench, S. R.; Carter, B. J.
- Science, Vol. 322, Issue 5904
Unicellular Cyanobacterium Symbiotic with a Single-Celled Eukaryotic Alga
journal, September 2012
- Thompson, A. W.; Foster, R. A.; Krupke, A.
- Science, Vol. 337, Issue 6101
Direct Measurement of O2-Depleted Microzones in Marine Oscillatoria: Relation to N2 Fixation
journal, July 1988
- Paerl, H. W.; Bebout, B. M.
- Science, Vol. 241, Issue 4864
Denitrifying Alphaproteobacteria from the Arabian Sea That Express nosZ , the Gene Encoding Nitrous Oxide Reductase, in Oxic and Suboxic Waters
journal, February 2013
- Wyman, Michael; Hodgson, Sylvia; Bird, Clare
- Applied and Environmental Microbiology, Vol. 79, Issue 8
Oxygen-Poor Microzones as Potential Sites of Microbial N2 Fixation in Nitrogen-Depleted Aerobic Marine Waters
journal, January 1987
- Paerl, Hans W.; Prufert, Leslie E.
- Applied and Environmental Microbiology, Vol. 53, Issue 5
Diazotrophy and Nitrogenase Activity in the Archaebacterium Methanosarcina barkeri 227
journal, January 1988
- Lobo, Anthony L.; Zinder, Stephen H.
- Applied and Environmental Microbiology, Vol. 54, Issue 7
Diazotrophic Diversity and Distribution in the Tropical and Subtropical Atlantic Ocean
journal, December 2005
- Langlois, R. J.; LaRoche, J.; Raab, P. A.
- Applied and Environmental Microbiology, Vol. 71, Issue 12
Unicellular, aerobic nitrogen-fixing cyanobacteria of the genus Cyanothece.
journal, March 1993
- Reddy, K. J.; Haskell, J. B.; Sherman, D. M.
- Journal of Bacteriology, Vol. 175, Issue 5
The metagenomics RAST server – a public resource for the automatic phylogenetic and functional analysis of metagenomes
journal, September 2008
- Meyer, F.; Paarmann, D.; D'Souza, M.
- BMC Bioinformatics, Vol. 9, Issue 1
Accelerated Profile HMM Searches
journal, October 2011
- Eddy, Sean R.
- PLoS Computational Biology, Vol. 7, Issue 10
FastTree 2 – Approximately Maximum-Likelihood Trees for Large Alignments
journal, March 2010
- Price, Morgan N.; Dehal, Paramvir S.; Arkin, Adam P.
- PLoS ONE, Vol. 5, Issue 3
Community Structure and Functional Gene Profile of Bacteria on Healthy and Diseased Thalli of the Red Seaweed Delisea pulchra
journal, December 2012
- Fernandes, Neil; Steinberg, Peter; Rusch, Doug
- PLoS ONE, Vol. 7, Issue 12
Discovery of an Endosymbiotic Nitrogen-Fixing Cyanobacterium UCYN-A in Braarudosphaera bigelowii (Prymnesiophyceae)
journal, December 2013
- Hagino, Kyoko; Onuma, Ryo; Kawachi, Masanobu
- PLoS ONE, Vol. 8, Issue 12
Anoxic aggregates - an ephemeral phenomenon in the pelagic environment?
journal, January 1997
- Ploug, H.; Kühl, M.; Buchholz-Cleven, B.
- Aquatic Microbial Ecology, Vol. 13
Evidence and a conceptual model for the co‑occurrence of nitrogen fixation and denitrification in heterotrophic marine sediments
journal, May 2013
- Fulweiler, Rw; Brown, Sm; Nixon, Sw
- Marine Ecology Progress Series, Vol. 482
No sign of denitrification in a Baltic Sea cyanobacterial bloom
journal, January 2002
- Hietanen, S.; Moisander, Ph; Kuparinen, J.
- Marine Ecology Progress Series, Vol. 242
Photosynthetic characteristics of Trichodesmium in the southwest Pacific Ocean: importance and significance
journal, May 2007
- Masotti, I.; Ruiz-Pino, D.; Le Bouteiller, A.
- Marine Ecology Progress Series, Vol. 338
Constitutive Extracellular Polysaccharide (EPS) Production by Specific Isolates of Crocosphaera watsonii
journal, January 2011
- Sohm, Jill A.; Edwards, Bethanie R.; Wilson, Brian G.
- Frontiers in Microbiology, Vol. 2
Two Strains of Crocosphaera watsonii with Highly Conserved Genomes are Distinguished by Strain-Specific Features
journal, January 2011
- Bench, Shellie R.; Ilikchyan, Irina N.; Tripp, H. James
- Frontiers in Microbiology, Vol. 2
Nitrogen fixation by algae in a Massachusetts salt marsh 1: N
journal, March 1978
- Carpenter, Edward J.; Van Raalte, Charlene D.; Valiela, Ivan
- Limnology and Oceanography, Vol. 23, Issue 2
Primary production of microalgae in sediments measured by oxygen microprofile, H 14 CO 3 - fixation, and oxygen exchange methods1 : Primary production of sediments
journal, July 1981
- Revsbech, Niels Peter; JØrgensen, Bo Barker; Brix, Ole
- Limnology and Oceanography, Vol. 26, Issue 4
Nitrogen fixation in freshwater, estuarine, and marine ecosystems. 1. Rates and importance
journal, January 1988
- Howarth, Robert W.; Marino, Roxanne; Lane, Judith
- Limnology and Oceanography, Vol. 33, Issue 4_part_2
Phylogenetic diversity of aggregate-attached vs. free-living marine bacterial assemblages
journal, July 1993
- DeLong, Edward F.; Franks, Diana G.; Alldredge, Alice L.
- Limnology and Oceanography, Vol. 38, Issue 5
Direct measurement of diffusivity within diatom aggregates containing transparent exopolymer particles
journal, January 2007
- Ploug, Helle; Passow, Uta
- Limnology and Oceanography, Vol. 52, Issue 1
Experiments linking nitrogenase gene expression to nitrogen fixation in the North Pacific subtropical gyre
journal, January 2007
- Zehr, Jonathan P.; Montoya, Joseph P.; Jenkins, Bethany D.
- Limnology and Oceanography, Vol. 52, Issue 1
Influence of the Amazon River plume on distributions of free-living and symbiotic cyanobacteria in the western tropical north Atlantic Ocean
journal, March 2007
- Foster, R. A.; Subramaniam, A.; Mahaffey, C.
- Limnology and Oceanography, Vol. 52, Issue 2
Cyanobacterial surface blooms formed by Aphanizomenon sp. and Nodularia spumigena in the Baltic Sea: Small-scale fluxes, pH, and oxygen microenvironments
journal, May 2008
- Ploug, Helle
- Limnology and Oceanography, Vol. 53, Issue 3
Influence of the Amazon River plume on distributions of free-living and symbiotic cyanobacteria in the western tropical north Atlantic Ocean
text, January 2007
- Foster, R. A.; Subramaniam, Ajit; Mahaffey, C.
- Columbia University
Works referencing / citing this record:
Diverse diazotrophs are present on sinking particles in the North Pacific Subtropical Gyre
journal, August 2018
- Farnelid, Hanna; Turk-Kubo, Kendra; Ploug, Helle
- The ISME Journal, Vol. 13, Issue 1
Diverse diazotrophs are present on sinking particles in the North Pacific Subtropical Gyre
journal, August 2018
- Farnelid, Hanna; Turk-Kubo, Kendra; Ploug, Helle
- The ISME Journal, Vol. 13, Issue 1