Metagenomic insights into diazotrophic communities across Arctic glacier forefields
Abstract
Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes.
- Authors:
-
- School of Geographical Sciences, University of Bristol, UK
- School of Life Sciences, University of Bristol, UK
- DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, US
- GFZ German Research Centre for Geosciences, Telegrafenenberg, 14473 Potsdam, Germany, School of Earth and Environment, University of Leeds, LS2 9JT, Leeds, UK, Department of Earth Sciences, Free University of Berlin, Malteserstr, 74-100, Building A, 12249, Berlin, Germany
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC); Univ. of California, Oakland, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1461009
- Alternate Identifier(s):
- OSTI ID: 1543956
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Published Article
- Journal Name:
- FEMS Microbiology Ecology (Online)
- Additional Journal Information:
- Journal Name: FEMS Microbiology Ecology (Online) Journal Volume: 94 Journal Issue: 9; Journal ID: ISSN 1574-6941
- Publisher:
- Federation of European Microbiological Societies
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Microbiology; nitrogen fixation; metagenomics; forefield; Arctic; diversity; diazotrophs
Citation Formats
Nash, Maisie V., Anesio, Alexandre M., Barker, Gary, Tranter, Martyn, Varliero, Gilda, Eloe-Fadrosh, Emiley A., Nielsen, Torben, Turpin-Jelfs, Thomas, Benning, Liane G., and Sánchez-Baracaldo, Patricia. Metagenomic insights into diazotrophic communities across Arctic glacier forefields. Netherlands: N. p., 2018.
Web. doi:10.1093/femsec/fiy114.
Nash, Maisie V., Anesio, Alexandre M., Barker, Gary, Tranter, Martyn, Varliero, Gilda, Eloe-Fadrosh, Emiley A., Nielsen, Torben, Turpin-Jelfs, Thomas, Benning, Liane G., & Sánchez-Baracaldo, Patricia. Metagenomic insights into diazotrophic communities across Arctic glacier forefields. Netherlands. https://doi.org/10.1093/femsec/fiy114
Nash, Maisie V., Anesio, Alexandre M., Barker, Gary, Tranter, Martyn, Varliero, Gilda, Eloe-Fadrosh, Emiley A., Nielsen, Torben, Turpin-Jelfs, Thomas, Benning, Liane G., and Sánchez-Baracaldo, Patricia. Tue .
"Metagenomic insights into diazotrophic communities across Arctic glacier forefields". Netherlands. https://doi.org/10.1093/femsec/fiy114.
@article{osti_1461009,
title = {Metagenomic insights into diazotrophic communities across Arctic glacier forefields},
author = {Nash, Maisie V. and Anesio, Alexandre M. and Barker, Gary and Tranter, Martyn and Varliero, Gilda and Eloe-Fadrosh, Emiley A. and Nielsen, Torben and Turpin-Jelfs, Thomas and Benning, Liane G. and Sánchez-Baracaldo, Patricia},
abstractNote = {Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes.},
doi = {10.1093/femsec/fiy114},
journal = {FEMS Microbiology Ecology (Online)},
number = 9,
volume = 94,
place = {Netherlands},
year = {Tue Jun 12 00:00:00 EDT 2018},
month = {Tue Jun 12 00:00:00 EDT 2018}
}
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Figures / Tables:
Table 1: Summary statistics for TN and TOC across the four fore-fields (Midtre Lovenbreen Ml, Russell Rl, Storglaciaren St and Rabots Rb). The average, minimum, maximum and standard deviation (SD) across each fore-field is given. The detection limit for both TN and TOC was 0.1 mg g−1. Sites recording valuesmore »
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