Adaptation of microscopy with ultraviolet surface excitation for enhancing STEM and undergraduate education

Huang, Chi Z.  R.; Wood, Ronald W.; Demos, Stavros G.

doi:10.1117/1.jbo.23.12.121603

Title: Adaptation of microscopy with ultraviolet surface excitation for enhancing STEM and undergraduate education

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Abstract

Microscopy with ultraviolet surface excitation (MUSE) is investigated as a means to enhance curricula and education in the life sciences based on simplicity of use, the incorporation of inexpensive hardware, and the simplest methods of tissue preparation. Ultraviolet excitation in effect replaces tissue sectioning because it penetrates only a few micrometers below the tissue surface at the single cell level, preventing the generation of out-of-focus light. Although tissue autofluorescence may be used, image quality and content can be enhanced by a brief immersion in a solution of nontoxic fluorescent dyes that selectively highlight different cellular compartments. Safe mixed-dye powder combinations have been developed to provide students who have minimal lab proficiencies with a one-step tissue staining process for rapid tissue preparation.

Authors:

Huang, Chi Z. R. ^[1]; Wood, Ronald W. ^[2]; Demos, Stavros G. ^[3]

Univ. of Rochester, NY (United States). Dept. of Biomedical Engineering. Lab. for Laser Energetics
Univ. of Rochester, NY (United States). School of Medicine and Dentistry. Depts. of Obstetrics and Gynecology, Urology, and Neuroscience
Univ. of Rochester, NY (United States). Lab. for Laser Energetics

Publication Date:: Fri Jul 27 00:00:00 EDT 2018

Research Org.:: Univ. of Rochester, NY (United States)

Sponsoring Org.:: USDOE National Nuclear Security Administration (NNSA); USDOE Advanced Research Projects Agency - Energy (ARPA-E)

OSTI Identifier:: 1464124

Grant/Contract Number:: NA0001944; AR0000568

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Biomedical Optics

Additional Journal Information:: Journal Volume: 23; Journal Issue: 12; Journal ID: ISSN 1083-3668

Publisher:: SPIE

Country of Publication:: United States

Language:: English

Subject:: 47 OTHER INSTRUMENTATION; imaging systems; fluorescence microscopy; medical and biological imaging; tissue characterization

Citation Formats


                    Huang, Chi Z.  R., Wood, Ronald W., and Demos, Stavros G. Adaptation of microscopy with ultraviolet surface excitation for enhancing STEM and undergraduate education.  United States: N. p., 2018. 
Web.  doi:10.1117/1.jbo.23.12.121603.

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                    Huang, Chi Z.  R., Wood, Ronald W., & Demos, Stavros G. Adaptation of microscopy with ultraviolet surface excitation for enhancing STEM and undergraduate education.  United States.  https://doi.org/10.1117/1.jbo.23.12.121603

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                    Huang, Chi Z.  R., Wood, Ronald W., and Demos, Stavros G. Fri .  
"Adaptation of microscopy with ultraviolet surface excitation for enhancing STEM and undergraduate education".  United States.  https://doi.org/10.1117/1.jbo.23.12.121603.  https://www.osti.gov/servlets/purl/1464124.

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@article{osti_1464124,

  title        = {Adaptation of microscopy with ultraviolet surface excitation for enhancing STEM and undergraduate education},

  author       = {Huang, Chi Z.  R. and Wood, Ronald W. and Demos, Stavros G.},

  abstractNote = {Microscopy with ultraviolet surface excitation (MUSE) is investigated as a means to enhance curricula and education in the life sciences based on simplicity of use, the incorporation of inexpensive hardware, and the simplest methods of tissue preparation. Ultraviolet excitation in effect replaces tissue sectioning because it penetrates only a few micrometers below the tissue surface at the single cell level, preventing the generation of out-of-focus light. Although tissue autofluorescence may be used, image quality and content can be enhanced by a brief immersion in a solution of nontoxic fluorescent dyes that selectively highlight different cellular compartments. Safe mixed-dye powder combinations have been developed to provide students who have minimal lab proficiencies with a one-step tissue staining process for rapid tissue preparation.},

  doi          = {10.1117/1.jbo.23.12.121603},

  journal      = {Journal of Biomedical Optics},

  number       = 12,

  volume       = 23,

  place        = {United States},

  year         = {Fri Jul 27 00:00:00 EDT 2018},

  month        = {Fri Jul 27 00:00:00 EDT 2018}

}

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