skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

This content will become publicly available on March 4, 2020

Title: Microscopy with ultraviolet surface excitation (MUSE) enables translation of optical biopsy principles to enhance life science education

Abstract

The translation of microscopy with ultraviolet surface excitation (MUSE) into a high school science classroom is investigated with the goal of providing a suitable new modality to enhance life science education. A key part of this effort is the development of laboratory exercises that can integrate the advanced capabilities of MUSE into a classroom setting. MUSE utilizes the unique property of ultraviolet light at wavelengths between 250 and 285 nm to propagate about 10 um into tissues, thus illuminating only the top cell layer. Furthermore this illumination is provided by a low-power UV LED source, which enables one to cost-efficiently implement this method into the educational environment. MUSE in education can eliminate the need for premade microscope slides and provide a far more engaging and rewarding experience for students.

Authors:
 [1];  [1];  [1]
  1. Univ. of Rochester, Rochester, NY (United States)
Publication Date:
Research Org.:
Univ. of Rochester, Rochester, NY (United States). Lab. for Laser Energetics
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA)
Contributing Org.:
Laboratory for Laser Energetics, University of Rochester
OSTI Identifier:
1502408
Report Number(s):
2018-268, 1479
2018-268, 1479, 2438
Grant/Contract Number:  
NA0003856
Resource Type:
Accepted Manuscript
Journal Name:
Proc. SPIE
Additional Journal Information:
Journal Volume: 10873; Conference: Photonics West, San Francisco, CA (United States), 2-7 Feb 2019
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; fluorescence microscope; ultraviolet fluorescence microscopy; curriculum; education; MUSE microscopy

Citation Formats

Kopp, Katherine A., Demos, Stavros G., and Kosc, Tanya Z. Microscopy with ultraviolet surface excitation (MUSE) enables translation of optical biopsy principles to enhance life science education. United States: N. p., 2019. Web. doi:10.1117/12.2515366.
Kopp, Katherine A., Demos, Stavros G., & Kosc, Tanya Z. Microscopy with ultraviolet surface excitation (MUSE) enables translation of optical biopsy principles to enhance life science education. United States. doi:10.1117/12.2515366.
Kopp, Katherine A., Demos, Stavros G., and Kosc, Tanya Z. Mon . "Microscopy with ultraviolet surface excitation (MUSE) enables translation of optical biopsy principles to enhance life science education". United States. doi:10.1117/12.2515366.
@article{osti_1502408,
title = {Microscopy with ultraviolet surface excitation (MUSE) enables translation of optical biopsy principles to enhance life science education},
author = {Kopp, Katherine A. and Demos, Stavros G. and Kosc, Tanya Z.},
abstractNote = {The translation of microscopy with ultraviolet surface excitation (MUSE) into a high school science classroom is investigated with the goal of providing a suitable new modality to enhance life science education. A key part of this effort is the development of laboratory exercises that can integrate the advanced capabilities of MUSE into a classroom setting. MUSE utilizes the unique property of ultraviolet light at wavelengths between 250 and 285 nm to propagate about 10 um into tissues, thus illuminating only the top cell layer. Furthermore this illumination is provided by a low-power UV LED source, which enables one to cost-efficiently implement this method into the educational environment. MUSE in education can eliminate the need for premade microscope slides and provide a far more engaging and rewarding experience for students.},
doi = {10.1117/12.2515366},
journal = {Proc. SPIE},
number = ,
volume = 10873,
place = {United States},
year = {2019},
month = {3}
}

Journal Article:
Free Publicly Available Full Text
This content will become publicly available on March 4, 2020
Publisher's Version of Record

Save / Share:

Works referenced in this record:

Characterizing the origin of autofluorescence in human esophageal epithelium under ultraviolet excitation
journal, January 2010

  • Lin, Bevin; Urayama, Shiro; Saroufeem, Ramez M. G.
  • Optics Express, Vol. 18, Issue 20, Article No. 21074
  • DOI: 10.1364/OE.18.021074