A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion
Abstract
Microbial aromatic catabolism offers a promising approach to convert lignin, a vast source of renewable carbon, into useful products. Aryl-O-demethylation is an essential biochemical reaction to ultimately catabolize coniferyl and sinapyl lignin-derived aromatic compounds, and is often a key bottleneck for both native and engineered bioconversion pathways. Here, we report the comprehensive characterization of a promiscuous P450 aryl-O-demethylase, consisting of a cytochrome P450 protein from the family CYP255A (GcoA) and a three-domain reductase (GcoB) that together represent a new two-component P450 class. Though originally described as converting guaiacol to catechol, we show that this system efficiently demethylates both guaiacol and an unexpectedly wide variety of lignin-relevant monomers. Here, structural, biochemical, and computational studies of this novel two-component system elucidate the mechanism of its broad substrate specificity, presenting it as a new tool for a critical step in biological lignin conversion.
- Authors:
-
[2];
[4];
[6];
[7];
[6];
[8];
[6];
[1]
- Univ. of Portsmouth, Portsmouth (United Kingdom)
- National Renewable Energy Lab. (NREL), Golden, CO (United States); Montana State Univ., Bozeman, MT (United States)
- National Renewable Energy Lab. (NREL), Golden, CO (United States); Univ. of Campinas, Sao Paulo (Brazil)
- Univ. of California at Los Angeles, Los Angeles, CA (United States)
- National Renewable Energy Lab. (NREL), Golden, CO (United States); Univ. of California at Los Angeles, Los Angeles, CA (United States)
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Univ. of Campinas, Sao Paulo (Brazil)
- Univ. of Georgia, Athens, GA (United States)
- Montana State Univ., Bozeman, MT (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office
- OSTI Identifier:
- 1458818
- Report Number(s):
- NREL/JA-5100-71206
Journal ID: ISSN 2041-1723
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; enzyme discovery; protein family; enzyme characterization; lignin conversion
Citation Formats
Mallinson, Sam J. B., Machovina, Melodie M., Silveira, Rodrigo L., Garcia-Borras, Marc, Gallup, Nathan, Johnson, Christopher W., Allen, Mark D., Skaf, Munir S., Crowley, Michael F., Neidle, Ellen L., Houk, Kendall N., Beckham, Gregg T., DuBois, Jennifer L., and McGeehan, John E. A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion. United States: N. p., 2018.
Web. doi:10.1038/s41467-018-04878-2.
Mallinson, Sam J. B., Machovina, Melodie M., Silveira, Rodrigo L., Garcia-Borras, Marc, Gallup, Nathan, Johnson, Christopher W., Allen, Mark D., Skaf, Munir S., Crowley, Michael F., Neidle, Ellen L., Houk, Kendall N., Beckham, Gregg T., DuBois, Jennifer L., & McGeehan, John E. A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion. United States. https://doi.org/10.1038/s41467-018-04878-2
Mallinson, Sam J. B., Machovina, Melodie M., Silveira, Rodrigo L., Garcia-Borras, Marc, Gallup, Nathan, Johnson, Christopher W., Allen, Mark D., Skaf, Munir S., Crowley, Michael F., Neidle, Ellen L., Houk, Kendall N., Beckham, Gregg T., DuBois, Jennifer L., and McGeehan, John E. Wed .
"A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion". United States. https://doi.org/10.1038/s41467-018-04878-2. https://www.osti.gov/servlets/purl/1458818.
@article{osti_1458818,
title = {A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion},
author = {Mallinson, Sam J. B. and Machovina, Melodie M. and Silveira, Rodrigo L. and Garcia-Borras, Marc and Gallup, Nathan and Johnson, Christopher W. and Allen, Mark D. and Skaf, Munir S. and Crowley, Michael F. and Neidle, Ellen L. and Houk, Kendall N. and Beckham, Gregg T. and DuBois, Jennifer L. and McGeehan, John E.},
abstractNote = {Microbial aromatic catabolism offers a promising approach to convert lignin, a vast source of renewable carbon, into useful products. Aryl-O-demethylation is an essential biochemical reaction to ultimately catabolize coniferyl and sinapyl lignin-derived aromatic compounds, and is often a key bottleneck for both native and engineered bioconversion pathways. Here, we report the comprehensive characterization of a promiscuous P450 aryl-O-demethylase, consisting of a cytochrome P450 protein from the family CYP255A (GcoA) and a three-domain reductase (GcoB) that together represent a new two-component P450 class. Though originally described as converting guaiacol to catechol, we show that this system efficiently demethylates both guaiacol and an unexpectedly wide variety of lignin-relevant monomers. Here, structural, biochemical, and computational studies of this novel two-component system elucidate the mechanism of its broad substrate specificity, presenting it as a new tool for a critical step in biological lignin conversion.},
doi = {10.1038/s41467-018-04878-2},
journal = {Nature Communications},
number = 1,
volume = 9,
place = {United States},
year = {Wed Jun 27 00:00:00 EDT 2018},
month = {Wed Jun 27 00:00:00 EDT 2018}
}
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