Discovery, characterization, and metabolic engineering of Rieske non-heme iron monooxygenases for guaiacol O-demethylation

Bleem, Alissa; Kuatsjah, Eugene; Presley, Gerald N.; Hinchen, Daniel J.; Zahn, Michael; Garcia, David C.; Michener, William E.; König, Gerhard; Tornesakis, Konstantinos; Allemann, Marco N.; Giannone, Richard J.; McGeehan, John E.; Beckham, Gregg T.; Michener, Joshua K.

doi:10.1016/j.checat.2022.04.019

Title: Discovery, characterization, and metabolic engineering of Rieske non-heme iron monooxygenases for guaiacol O-demethylation

Journal Article · 20 May 2022 · Chem Catalysis

DOI: https://doi.org/10.1016/j.checat.2022.04.019 · OSTI ID:1873828

Bleem, Alissa ^[1]; Kuatsjah, Eugene ^[1]; Presley, Gerald N. ^[2]; Hinchen, Daniel J. ^[3]; Zahn, Michael ^[3]; Garcia, David C. ^[4]; Michener, William E. ^[5]; König, Gerhard ^[3]; Tornesakis, Konstantinos ^[3]; Allemann, Marco N. ^[6];

^[2]; McGeehan, John E. ^[3]; Beckham, Gregg T. ^[1];

^[2]

National Renewable Energy Lab. (NREL), Golden, CO (United States). Renewable Resources and Enabling Science Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Bioenergy Innovation (CBI)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Bioenergy Innovation (CBI); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of Portsmouth (United Kingdom). Inst. of Biological and Biomedical Sciences, Center for Enzyme Innovation
Univ. of Tennessee, Knoxville, TN (United States). Bredesen Center for Interdisciplinary Research and Graduate Education
National Renewable Energy Lab. (NREL), Golden, CO (United States). Renewable Resources and Enabling Science Center
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Aryl-O-demethylation is a common rate-limiting step in the catabolism of lignin-related compounds, including guaiacol. In this work, we used randomly barcoded transposon insertion sequencing (RB-TnSeq) in the bacterium Novosphingobium aromaticivorans to identify a Rieske-type guaiacol O-demethylase, GdmA. Similarity searches identified GdmA homologs in other bacteria, along with candidate reductase partners, denoted GdmB. GdmAB combinations were biochemically characterized for activity with several lignin-related substrates. Structural and sequence comparisons of vanillate- and guaiacol-specific O-demethylase active sites revealed conserved hallmarks of substrate specificity. GdmAB combinations were also evaluated in Pseudomonas putida KT2440, which does not natively utilize guaiacol. GdmAB from Cupriavidus necator N-1 demonstrated the highest rate of guaiacol turnover in vitro and in engineered P. putida strains and notably higher catalytic efficiency than a cytochrome P450 system (GcoAB) and the vanillate Rieske-type O-demethylase from P. putida (VanAB). The GdmAB O-demethylases described here expand the suite of options for microbial conversion of a model lignin-derived substrate.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725; AC36-08GO28308

OSTI ID:: 1873828

Journal Information:: Chem Catalysis, Journal Name: Chem Catalysis Journal Issue: 8 Vol. 2; ISSN 2667-1093

Publisher:: Cell PressCopyright Statement

Country of Publication:: United States

Language:: English

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