Engineering Elastin-Like Polypeptide-Poly(ethylene glycol) Multiblock Physical Networks

Araújo, Andreia; Olsen, Bradley D.; Machado, Ana Vera

doi:10.1021/acs.biomac.7b01424

Title: Engineering Elastin-Like Polypeptide-Poly(ethylene glycol) Multiblock Physical Networks

Abstract

Hybrids of protein biopolymers and synthetic polymers are a promising new class of soft materials, as the advantages of each component can be complementary. A recombinant elastin-like polypeptide (ELP) was conjugated to poly(ethylene glycol) (PEG) by macromolecular coupling in solution to form multiblock ELP-PEG copolymers. The hydrated copolymer preserved the thermoresponsive properties from the ELP block and formed hydrogels with different transition temperatures depending on salt concentration. Small angle scattering indicates that the copolymer hydrogels form sphere-like aggregates with a “fuzzy” interface, while the films form a fractal network of nanoscale aggregates. Here, the use of solutions with different salt concentrations to prepare the hydrogels was found to influence the transition temperature, the mechanical properties, and the size of the nanoscale structure of the hydrogel without changing the secondary structure of the ELP. The salt variation and the addition of a plasticizer also affected the nanoscale structure and the mechanical characteristics of the films.

Authors:

Araújo, Andreia ^[1];

^[2];

^[1]

Univ. of Minho, Guimarães (Portugal)
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Publication Date:: Mon Dec 18 00:00:00 EST 2017

Research Org.:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Org.:: National Science Foundation (NSF)

OSTI Identifier:: 1437484

Resource Type:: Accepted Manuscript

Journal Name:: Biomacromolecules

Additional Journal Information:: Journal Volume: 19; Journal Issue: 2; Journal ID: ISSN 1525-7797

Publisher:: American Chemical Society

Country of Publication:: United States

Language:: ENGLISH

Subject:: 59 BASIC BIOLOGICAL SCIENCES; thin films; amides; peptides and proteins; copolymers; hydrogels; PEG; ELP; physical networks

Citation Formats


                    Araújo, Andreia, Olsen, Bradley D., and Machado, Ana Vera. Engineering Elastin-Like Polypeptide-Poly(ethylene glycol) Multiblock Physical Networks.  United States: N. p., 2017. 
Web.  doi:10.1021/acs.biomac.7b01424.

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                    Araújo, Andreia, Olsen, Bradley D., & Machado, Ana Vera. Engineering Elastin-Like Polypeptide-Poly(ethylene glycol) Multiblock Physical Networks.  United States.  https://doi.org/10.1021/acs.biomac.7b01424

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                    Araújo, Andreia, Olsen, Bradley D., and Machado, Ana Vera. Mon .  
"Engineering Elastin-Like Polypeptide-Poly(ethylene glycol) Multiblock Physical Networks".  United States.  https://doi.org/10.1021/acs.biomac.7b01424.  https://www.osti.gov/servlets/purl/1437484.

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@article{osti_1437484,

  title        = {Engineering Elastin-Like Polypeptide-Poly(ethylene glycol) Multiblock Physical Networks},

  author       = {Araújo, Andreia and Olsen, Bradley D. and Machado, Ana Vera},

  abstractNote = {Hybrids of protein biopolymers and synthetic polymers are a promising new class of soft materials, as the advantages of each component can be complementary. A recombinant elastin-like polypeptide (ELP) was conjugated to poly(ethylene glycol) (PEG) by macromolecular coupling in solution to form multiblock ELP-PEG copolymers. The hydrated copolymer preserved the thermoresponsive properties from the ELP block and formed hydrogels with different transition temperatures depending on salt concentration. Small angle scattering indicates that the copolymer hydrogels form sphere-like aggregates with a “fuzzy” interface, while the films form a fractal network of nanoscale aggregates. Here, the use of solutions with different salt concentrations to prepare the hydrogels was found to influence the transition temperature, the mechanical properties, and the size of the nanoscale structure of the hydrogel without changing the secondary structure of the ELP. The salt variation and the addition of a plasticizer also affected the nanoscale structure and the mechanical characteristics of the films.},

  doi          = {10.1021/acs.biomac.7b01424},

  journal      = {Biomacromolecules},

  number       = 2,

  volume       = 19,

  place        = {United States},

  year         = {Mon Dec 18 00:00:00 EST 2017},

  month        = {Mon Dec 18 00:00:00 EST 2017}

}

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Title: Engineering Elastin-Like Polypeptide-Poly(ethylene glycol) Multiblock Physical Networks

Abstract

Citation Formats

The mechanical efficiency of natural materials journal, July 2004

Utilizing Peptidic Ordering in the Design of Hierarchical Polyurethane/Ureas journal, April 2012

Engineering materials from proteins journal, September 2013

Bioinspired structural materials journal, October 2014

Poly(2-oxazoline)s as Smart Bioinspired Polymers: Poly(2-oxazoline)s as Smart Bioinspired Polymers journal, January 2010

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Engineering hybrid polymer-protein super-aligned nanofibers via rotary jet spinning journal, March 2014

Modular protein domains: an engineering approach toward functional biomaterials journal, August 2016

Synthesis and Application of Protein-Containing Block Copolymers journal, January 2015

Engineered Coiled-Coil Protein Microfibers journal, September 2014

Biological materials: Structure and mechanical properties journal, January 2008

Underwater Bonding with Charged Polymer Mimics of Marine Mussel Adhesive Proteins journal, July 2011

Coherently wired light-harvesting in photosynthetic marine algae at ambient temperature journal, February 2010

Laccases: blue enzymes for green chemistry journal, May 2006

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The synthetic polypentapeptide of elastin coacervates and forms filamentous aggregates journal, December 1974

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Self-Assembly of β-Sheets into Nanostructures by Poly(alanine) Segments Incorporated in Multiblock Copolymers Inspired by Spider Silk journal, June 2001

Synthesis and Characterization of Elastin−Mimetic Hybrid Polymers with Multiblock, Alternating Molecular Architecture and Elastomeric Properties journal, March 2009

Macromolecule-Induced Assembly of Coiled-Coils in Alternating Multiblock Polymers journal, September 2009

Bioactive hydrogels made from step-growth derived PEG–peptide macromers journal, May 2010

Tuning the Properties of Elastin Mimetic Hybrid Copolymers via a Modular Polymerization Method journal, May 2012

Arrested Phase Separation of Elastin-like Polypeptide Solutions Yields Stiff, Thermoresponsive Gels journal, November 2015

Toughening of Thermoresponsive Arrested Networks of Elastin-Like Polypeptides To Engineer Cytocompatible Tissue Scaffolds journal, January 2016

The Thiol-Michael Addition Click Reaction: A Powerful and Widely Used Tool in Materials Chemistry journal, September 2013

Differential scanning calorimetry studies on poly(ethylene glycol) with different molecular weights for thermal energy storage materials journal, October 2002

Rapid micropatterning by temperature-triggered reversible gelation of a recombinant smart elastin-like tetrablock-copolymer journal, January 2010

Obtaining information about protein secondary structures in aqueous solution using Fourier transform IR spectroscopy journal, February 2015

Electrophoretic Transport of Poly(ethylene glycol) Chains through Poly(acrylamide) Gel † journal, October 1999

Preparation and characterization of novel elastin-like polypeptide-collagen composites journal, February 2013

Structural investigation of the poly(pentapeptide) of elastin, poly(GVGVP), in the solid state journal, August 1999

Self-Assembly of PEGylated Peptide Conjugates Containing a Modified Amyloid β-Peptide Fragment journal, June 2010

Use of ionic liquid and microwave irradiation as a convenient, rapid and eco-friendly method for synthesis of novel optically active and thermally stable aromatic polyamides containing N-phthaloyl-l-alanine pendent group journal, April 2008

Fourier Transform Infrared Spectroscopic Analysis of Protein Secondary Structures journal, August 2007

Synthesis and Characterization of Polyethylene Glycol Mediated Silver Nanoparticles by the Green Method journal, May 2012

Infrared spectroscopy of proteins journal, September 2007

An Infrared Spectroscopic Study of the Conformational Transition of Elastin-Like Polypeptides journal, October 2007

Recombinant elastin-mimetic biomaterials: Emerging applications in medicine journal, December 2010

Thermoresponsive hybrid hydrogel of oxidized nanocellulose using a polypeptide crosslinker journal, February 2014

Optimization of ELP-intein mediated protein purification by salt substitution journal, August 2009

Self-assembly of block copolymers derived from elastin-mimetic polypeptide sequences journal, October 2002

The structural properties of a two-Yukawa fluid: Simulation and analytical results journal, February 2006

Rheological properties of peptide-based hydrogels for biomedical and other applications journal, January 2010

Protein polymer hydrogels by in situ, rapid and reversible self-gelation journal, July 2012

Stimuli-Responsive Thin Coatings Using Elastin-Like Polymers for Biomedical Applications journal, October 2009

Cell Response to RGD Density in Cross-Linked Artificial Extracellular Matrix Protein Films journal, October 2008

Physical properties of artificial extracellular matrix protein films prepared by isocyanate crosslinking journal, March 2004

Micro- and nano-structural characterization of six marine sponges of the class Demospongiae journal, April 2016

Multifunctional elastin-like polypeptide renders β-glucosidase enzyme phase transition and high stability journal, June 2019

Multifunctional elastin-like polypeptide renders β-glucosidase enzyme phase transition and high stability journal, June 2019

The mechanical efficiency of natural materials
journal, July 2004

Utilizing Peptidic Ordering in the Design of Hierarchical Polyurethane/Ureas
journal, April 2012

Engineering materials from proteins
journal, September 2013

Bioinspired structural materials
journal, October 2014

Poly(2-oxazoline)s as Smart Bioinspired Polymers: Poly(2-oxazoline)s as Smart Bioinspired Polymers
journal, January 2010

A Supramolecular-Hydrogel-Encapsulated Hemin as an Artificial Enzyme to Mimic Peroxidase
journal, June 2007

Engineering hybrid polymer-protein super-aligned nanofibers via rotary jet spinning
journal, March 2014

Modular protein domains: an engineering approach toward functional biomaterials
journal, August 2016

Synthesis and Application of Protein-Containing Block Copolymers
journal, January 2015

Engineered Coiled-Coil Protein Microfibers
journal, September 2014

Biological materials: Structure and mechanical properties
journal, January 2008

Underwater Bonding with Charged Polymer Mimics of Marine Mussel Adhesive Proteins
journal, July 2011

Coherently wired light-harvesting in photosynthetic marine algae at ambient temperature
journal, February 2010

Laccases: blue enzymes for green chemistry
journal, May 2006

A General Strategy to Convert the MerR Family Proteins into Highly Sensitive and Selective Fluorescent Biosensors for Metal Ions
journal, January 2004

The synthetic polypentapeptide of elastin coacervates and forms filamentous aggregates
journal, December 1974

Self-aggregation characteristics of recombinantly expressed human elastin polypeptides
journal, November 2001

Genetically Encoded Synthesis of Protein-Based Polymers with Precisely Specified Molecular Weight and Sequence by Recursive Directional Ligation: Examples from the Elastin-like Polypeptide System
journal, March 2002

Hydrazone self-crosslinking of multiphase elastin-like block copolymer networks
journal, March 2012

Chondrocytic differentiation of human adipose-derived adult stem cells in elastin-like polypeptide
journal, January 2006

Secondary Structure Formation and LCST Behavior of Short Elastin-Like Peptides
journal, August 2008

Swelling and Mechanical Behaviors of Chemically Cross-Linked Hydrogels of Elastin-like Polypeptides
journal, May 2003

In Situ Cross-Linking of Elastin-like Polypeptide Block Copolymers for Tissue Repair
journal, December 2007

Elastin as a biomaterial for tissue engineering
journal, October 2007

Protein- and peptide-modified synthetic polymeric biomaterials
journal, January 2010

Peptide-Polymer Conjugates: From Fundamental Science to Application
journal, April 2013

Therapeutic Protein–Polymer Conjugates: Advancing Beyond PEGylation
journal, September 2014

Smart hybrid materials by conjugation of responsive polymers to biomacromolecules
journal, November 2014

Thermoresponsive Self-Assembly of Short Elastin-Like Polypentapeptides and Their Poly(ethylene glycol) Derivatives
journal, January 2007

Elastin-Based Side-Chain Polymers Synthesized by ATRP
journal, August 2003

Self-Assembly of β-Sheets into Nanostructures by Poly(alanine) Segments Incorporated in Multiblock Copolymers Inspired by Spider Silk
journal, June 2001

Synthesis and Characterization of Elastin−Mimetic Hybrid Polymers with Multiblock, Alternating Molecular Architecture and Elastomeric Properties
journal, March 2009

Macromolecule-Induced Assembly of Coiled-Coils in Alternating Multiblock Polymers
journal, September 2009

Bioactive hydrogels made from step-growth derived PEG–peptide macromers
journal, May 2010

Tuning the Properties of Elastin Mimetic Hybrid Copolymers via a Modular Polymerization Method
journal, May 2012

Arrested Phase Separation of Elastin-like Polypeptide Solutions Yields Stiff, Thermoresponsive Gels
journal, November 2015

Toughening of Thermoresponsive Arrested Networks of Elastin-Like Polypeptides To Engineer Cytocompatible Tissue Scaffolds
journal, January 2016

The Thiol-Michael Addition Click Reaction: A Powerful and Widely Used Tool in Materials Chemistry
journal, September 2013

Differential scanning calorimetry studies on poly(ethylene glycol) with different molecular weights for thermal energy storage materials
journal, October 2002

Rapid micropatterning by temperature-triggered reversible gelation of a recombinant smart elastin-like tetrablock-copolymer
journal, January 2010

Obtaining information about protein secondary structures in aqueous solution using Fourier transform IR spectroscopy
journal, February 2015

Electrophoretic Transport of Poly(ethylene glycol) Chains through Poly(acrylamide) Gel ^†
journal, October 1999

Preparation and characterization of novel elastin-like polypeptide-collagen composites
journal, February 2013

Structural investigation of the poly(pentapeptide) of elastin, poly(GVGVP), in the solid state
journal, August 1999

Self-Assembly of PEGylated Peptide Conjugates Containing a Modified Amyloid β-Peptide Fragment
journal, June 2010

Use of ionic liquid and microwave irradiation as a convenient, rapid and eco-friendly method for synthesis of novel optically active and thermally stable aromatic polyamides containing N-phthaloyl-l-alanine pendent group
journal, April 2008

Fourier Transform Infrared Spectroscopic Analysis of Protein Secondary Structures
journal, August 2007

Synthesis and Characterization of Polyethylene Glycol Mediated Silver Nanoparticles by the Green Method
journal, May 2012

Infrared spectroscopy of proteins
journal, September 2007

An Infrared Spectroscopic Study of the Conformational Transition of Elastin-Like Polypeptides
journal, October 2007

Recombinant elastin-mimetic biomaterials: Emerging applications in medicine
journal, December 2010

Thermoresponsive hybrid hydrogel of oxidized nanocellulose using a polypeptide crosslinker
journal, February 2014

Optimization of ELP-intein mediated protein purification by salt substitution
journal, August 2009

Self-assembly of block copolymers derived from elastin-mimetic polypeptide sequences
journal, October 2002

The structural properties of a two-Yukawa fluid: Simulation and analytical results
journal, February 2006

Rheological properties of peptide-based hydrogels for biomedical and other applications
journal, January 2010

Protein polymer hydrogels by in situ, rapid and reversible self-gelation
journal, July 2012

Stimuli-Responsive Thin Coatings Using Elastin-Like Polymers for Biomedical Applications
journal, October 2009

Cell Response to RGD Density in Cross-Linked Artificial Extracellular Matrix Protein Films
journal, October 2008

Physical properties of artificial extracellular matrix protein films prepared by isocyanate crosslinking
journal, March 2004

Micro- and nano-structural characterization of six marine sponges of the class Demospongiae
journal, April 2016

Multifunctional elastin-like polypeptide renders β-glucosidase enzyme phase transition and high stability
journal, June 2019

Multifunctional elastin-like polypeptide renders β-glucosidase enzyme phase transition and high stability
journal, June 2019