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Title: A metallo-biopolymer conjugate of elastin-like polypeptide: photoluminescence enhancement in the coacervate microenvironment

Abstract

An optically active metallo-polymer assembly is demonstrated here via conjugation of a genetically engineered elastin-like polypeptide (ELP) and a ruthenium(II) polypyridyl complex. By taking advantage of the phase transition of ELPs in water, photophysical properties of the resultant conjugate are investigated for both phases, below and above the critical transition temperature. Upon coacervation, the luminescence of the metallo-ELP is greatly enhanced as a consequence of local effects on the metal–ligand luminophore. Finally, these findings open a possibility to harness the temperature control of stimuli-responsive properties of biopolymers.

Authors:
 [1];  [1];  [1];  [1];  [1]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Workforce Development for Teachers and Scientists (WDTS) (SC-27); USDOE National Nuclear Security Administration (NNSA); LANL Laboratory Directed Research and Development (LDRD) Program
OSTI Identifier:
1482928
Report Number(s):
LA-UR-18-21561
Journal ID: ISSN 0949-8257
Grant/Contract Number:  
AC52-06NA25396
Resource Type:
Accepted Manuscript
Journal Name:
JBIC Journal of Biological Inorganic Chemistry
Additional Journal Information:
Journal Volume: 23; Journal Issue: 7; Journal ID: ISSN 0949-8257
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; Biological Science; Inorganic and Physical Chemistry; Material Science

Citation Formats

Ghosh, Koushik, Elbert, Katherine C., Balog, Eva Rose M., Martinez, Jennifer S., and Rocha, Reginaldo C. A metallo-biopolymer conjugate of elastin-like polypeptide: photoluminescence enhancement in the coacervate microenvironment. United States: N. p., 2018. Web. doi:10.1007/s00775-018-1580-6.
Ghosh, Koushik, Elbert, Katherine C., Balog, Eva Rose M., Martinez, Jennifer S., & Rocha, Reginaldo C. A metallo-biopolymer conjugate of elastin-like polypeptide: photoluminescence enhancement in the coacervate microenvironment. United States. doi:10.1007/s00775-018-1580-6.
Ghosh, Koushik, Elbert, Katherine C., Balog, Eva Rose M., Martinez, Jennifer S., and Rocha, Reginaldo C. Fri . "A metallo-biopolymer conjugate of elastin-like polypeptide: photoluminescence enhancement in the coacervate microenvironment". United States. doi:10.1007/s00775-018-1580-6. https://www.osti.gov/servlets/purl/1482928.
@article{osti_1482928,
title = {A metallo-biopolymer conjugate of elastin-like polypeptide: photoluminescence enhancement in the coacervate microenvironment},
author = {Ghosh, Koushik and Elbert, Katherine C. and Balog, Eva Rose M. and Martinez, Jennifer S. and Rocha, Reginaldo C.},
abstractNote = {An optically active metallo-polymer assembly is demonstrated here via conjugation of a genetically engineered elastin-like polypeptide (ELP) and a ruthenium(II) polypyridyl complex. By taking advantage of the phase transition of ELPs in water, photophysical properties of the resultant conjugate are investigated for both phases, below and above the critical transition temperature. Upon coacervation, the luminescence of the metallo-ELP is greatly enhanced as a consequence of local effects on the metal–ligand luminophore. Finally, these findings open a possibility to harness the temperature control of stimuli-responsive properties of biopolymers.},
doi = {10.1007/s00775-018-1580-6},
journal = {JBIC Journal of Biological Inorganic Chemistry},
number = 7,
volume = 23,
place = {United States},
year = {2018},
month = {6}
}

Journal Article:
Free Publicly Available Full Text
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Figures / Tables:

Fig. 1 Fig. 1: Amino acid sequence of the polymer (ELP), chemical structure of the luminophore (Ru), and conjugation scheme (A = EDC/sulfo-NHS in phosphate buffer; pH 7.0).

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    Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.