Monitoring bacterial biofilms with a microfluidic flow chip designed for imaging with white-light interferometry
Abstract
There is a need for imaging and sensing instrumentation that can monitor transitions in biofilm structure in order to better understand biofilm development and emergent properties such as anti-microbial resistance. Herein, we expanded on our previously reported technique for measuring and monitoring the thickness and topology of live biofilms using white-light interferometry (WLI). A flow cell designed for WLI enabled the use of this non-disruptive imaging method for the capture of high resolution three-dimensional profile images of biofilm growth over time. The fine axial resolution (3 nm) and wide field of view (>1 mm by 1 mm) enabled detection of biofilm formation as early as three hours after inoculation of the flow cell with a live bacterial culture (Pseudomonas fluorescens). Additionally, WLI imaging facilitated monitoring the early stages of biofilm development and subtle variations in the structure of mature biofilms. Minimally-invasive imaging enabled monitoring of biofilm structure with surface metrology metrics (e.g., surface roughness). The system was used to observe a transition in biofilm structure that occurred in response to expsoure to a common antiseptic. In the future, WLI and the biofilm imaging cell described herein may be used to test the effectiveness of biofilm-specific therapies to combat common diseasesmore »
- Authors:
-
[2]
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Signatures Science and Technology Division
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1378001
- Report Number(s):
- PNNL-SA-126357
Journal ID: ISSN 1932-1058
- Grant/Contract Number:
- AC05-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biomicrofluidics
- Additional Journal Information:
- Journal Volume: 11; Journal Issue: 4; Journal ID: ISSN 1932-1058
- Publisher:
- American Institute of Physics (AIP)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE; 59 BASIC BIOLOGICAL SCIENCES; biofilm; flow cell; Interferometry; White light interferometry; Microscopy
Citation Formats
Brann, Michelle, Suter, Jonathan D., Addleman, R. Shane, and Larimer, Curtis. Monitoring bacterial biofilms with a microfluidic flow chip designed for imaging with white-light interferometry. United States: N. p., 2017.
Web. doi:10.1063/1.4985773.
Brann, Michelle, Suter, Jonathan D., Addleman, R. Shane, & Larimer, Curtis. Monitoring bacterial biofilms with a microfluidic flow chip designed for imaging with white-light interferometry. United States. https://doi.org/10.1063/1.4985773
Brann, Michelle, Suter, Jonathan D., Addleman, R. Shane, and Larimer, Curtis. Fri .
"Monitoring bacterial biofilms with a microfluidic flow chip designed for imaging with white-light interferometry". United States. https://doi.org/10.1063/1.4985773. https://www.osti.gov/servlets/purl/1378001.
@article{osti_1378001,
title = {Monitoring bacterial biofilms with a microfluidic flow chip designed for imaging with white-light interferometry},
author = {Brann, Michelle and Suter, Jonathan D. and Addleman, R. Shane and Larimer, Curtis},
abstractNote = {There is a need for imaging and sensing instrumentation that can monitor transitions in biofilm structure in order to better understand biofilm development and emergent properties such as anti-microbial resistance. Herein, we expanded on our previously reported technique for measuring and monitoring the thickness and topology of live biofilms using white-light interferometry (WLI). A flow cell designed for WLI enabled the use of this non-disruptive imaging method for the capture of high resolution three-dimensional profile images of biofilm growth over time. The fine axial resolution (3 nm) and wide field of view (>1 mm by 1 mm) enabled detection of biofilm formation as early as three hours after inoculation of the flow cell with a live bacterial culture (Pseudomonas fluorescens). Additionally, WLI imaging facilitated monitoring the early stages of biofilm development and subtle variations in the structure of mature biofilms. Minimally-invasive imaging enabled monitoring of biofilm structure with surface metrology metrics (e.g., surface roughness). The system was used to observe a transition in biofilm structure that occurred in response to expsoure to a common antiseptic. In the future, WLI and the biofilm imaging cell described herein may be used to test the effectiveness of biofilm-specific therapies to combat common diseases associated with biofilm formation such as cystic fibrosis and periodontitis.},
doi = {10.1063/1.4985773},
journal = {Biomicrofluidics},
number = 4,
volume = 11,
place = {United States},
year = {Fri Aug 18 00:00:00 EDT 2017},
month = {Fri Aug 18 00:00:00 EDT 2017}
}
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