Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics Setup
Abstract
Coupling microfluidics with microscopy has emerged as a powerful approach to study at cellular resolution the dynamics in plant physiology and root-microbe interactions (RMIs). Most devices have been designed to study the model plant Arabidopsis thaliana at higher throughput than conventional methods. However, there is a need for microfluidic devices which enable in vivo studies of root development and RMIs in woody plants. Here, we developed the RMI-chip, a simple microfluidic setup in which Populus tremuloides (aspen tree) seedlings can grow for over a month, allowing continuous microscopic observation of interactions between live roots and rhizobacteria. We find that the colonization of growing aspen roots by Pseudomonas fluorescens in the RMI-chip involves dynamic biofilm formation and dispersal, in keeping with previous observations in a different experimental set-up. Also, we find that whole-cell biosensors based on the rhizobacterium Bacillus subtilis can be used to monitor compositional changes in the rhizosphere but that the application of these biosensors is limited by their efficiency at colonizing aspen roots and persisting. These results indicate that functional imaging of dynamic root-bacteria interactions in the RMI-chip requires careful matching between the host plant and the bacterial root colonizer.
- Authors:
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1615031
- Alternate Identifier(s):
- OSTI ID: 1615760
- Grant/Contract Number:
- AC02-06CH11357
- Resource Type:
- Published Article
- Journal Name:
- Frontiers in Plant Science
- Additional Journal Information:
- Journal Name: Frontiers in Plant Science Journal Volume: 11; Journal ID: ISSN 1664-462X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- Switzerland
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Bacillus subtilis; Populus tremuloides; Pseudomonas fluorescens; biosensor; microfluidics; plant-microbe interactions; root-microbe interaction
Citation Formats
Noirot-Gros, Marie-Francoise, Shinde, Shalaka V., Akins, Chase, Johnson, Jessica L., Zerbs, Sarah, Wilton, Rosemarie, Kemner, Kenneth M., Noirot, Philippe, and Babnigg, Gyorgy. Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics Setup. Switzerland: N. p., 2020.
Web. doi:10.3389/fpls.2020.00408.
Noirot-Gros, Marie-Francoise, Shinde, Shalaka V., Akins, Chase, Johnson, Jessica L., Zerbs, Sarah, Wilton, Rosemarie, Kemner, Kenneth M., Noirot, Philippe, & Babnigg, Gyorgy. Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics Setup. Switzerland. https://doi.org/10.3389/fpls.2020.00408
Noirot-Gros, Marie-Francoise, Shinde, Shalaka V., Akins, Chase, Johnson, Jessica L., Zerbs, Sarah, Wilton, Rosemarie, Kemner, Kenneth M., Noirot, Philippe, and Babnigg, Gyorgy. Wed .
"Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics Setup". Switzerland. https://doi.org/10.3389/fpls.2020.00408.
@article{osti_1615031,
title = {Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics Setup},
author = {Noirot-Gros, Marie-Francoise and Shinde, Shalaka V. and Akins, Chase and Johnson, Jessica L. and Zerbs, Sarah and Wilton, Rosemarie and Kemner, Kenneth M. and Noirot, Philippe and Babnigg, Gyorgy},
abstractNote = {Coupling microfluidics with microscopy has emerged as a powerful approach to study at cellular resolution the dynamics in plant physiology and root-microbe interactions (RMIs). Most devices have been designed to study the model plant Arabidopsis thaliana at higher throughput than conventional methods. However, there is a need for microfluidic devices which enable in vivo studies of root development and RMIs in woody plants. Here, we developed the RMI-chip, a simple microfluidic setup in which Populus tremuloides (aspen tree) seedlings can grow for over a month, allowing continuous microscopic observation of interactions between live roots and rhizobacteria. We find that the colonization of growing aspen roots by Pseudomonas fluorescens in the RMI-chip involves dynamic biofilm formation and dispersal, in keeping with previous observations in a different experimental set-up. Also, we find that whole-cell biosensors based on the rhizobacterium Bacillus subtilis can be used to monitor compositional changes in the rhizosphere but that the application of these biosensors is limited by their efficiency at colonizing aspen roots and persisting. These results indicate that functional imaging of dynamic root-bacteria interactions in the RMI-chip requires careful matching between the host plant and the bacterial root colonizer.},
doi = {10.3389/fpls.2020.00408},
journal = {Frontiers in Plant Science},
number = ,
volume = 11,
place = {Switzerland},
year = {Wed Apr 15 00:00:00 EDT 2020},
month = {Wed Apr 15 00:00:00 EDT 2020}
}
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https://doi.org/10.3389/fpls.2020.00408
https://doi.org/10.3389/fpls.2020.00408
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Cited by: 18 works
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