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Title: Pseudomonas fluorescens transportome is linked to strain-specific plant growth promotion in Aspen seedlings under nutrient stress

Diverse communities of bacteria colonize plant roots and the rhizosphere. Many of these rhizobacteria are symbionts and provide plant growth promotion (PGP) services, protecting the plant from biotic and abiotic stresses and increasing plant productivity by providing access to nutrients that would otherwise be unavailable to roots. In return, these symbiotic bacteria receive photosynthetically-derived carbon (C), in the form of sugars and organic acids, from plant root exudates. PGP activities have been characterized for a variety of forest tree species and are important in C cycling and sequestration in terrestrial ecosystems. The molecular mechanisms of these PGP activities, however, are less well-known. In a previous analysis of Pseudomonas genomes, we found that the bacterial transportome, the aggregate activity of a bacteria's transmembrane transporters, was most predictive for the ecological niche of Pseudomonads in the rhizosphere. Here, we used Populus tremuloides Michx. (trembling aspen) seedlings inoculated with one of three Pseudomonas fluorescens strains (Pf0-1, SBW25, and WH6) and one Pseudomonas protegens (Pf-5) as a laboratory model to further investigate the relationships between the predicted transportomic capacity of a bacterial strain and its observed PGP effects in laboratory cultures. Conditions of low nitrogen (N) or low phosphorus (P) availability and the correspondingmore » replete media conditions were investigated. We measured phenotypic and biochemical parameters of P. tremuloides seedlings and correlated P fluorescens strain-specific transportomic capacities with P. tremuloides seedling phenotype to predict the strain and nutrient environment-specific transporter functions that lead to experimentally observed, strain, and media-specific PGP activities and the capacity to protect plants against nutrient stress. These predicted transportomic functions fall in three groups: (i) transport of compounds that modulate aspen seedling root architecture, (ii) transport of compounds that help to mobilize nutrients for aspen roots, and (iii) transporters that enable bacterial acquisition of C sources from seedling root exudates. Lastly, these predictions point to specific molecular mechanisms of PGP activities that can be directly tested through future, hypothesis-driven biological experiments.« less
Authors:
 [1] ;  [2] ;  [1] ;  [1] ;  [3]
  1. Argonne National Lab. (ANL), Lemont, IL (United States)
  2. West Virginia Univ., Morgantown, WV (United States)
  3. Argonne National Lab. (ANL), Lemont, IL (United States); Univ. of Illinois at Chicago, Chicago, IL (United States)
Publication Date:
Grant/Contract Number:
AC02-06CH11357
Type:
Accepted Manuscript
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
Journal Volume: 8; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Research Foundation
Research Org:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; plant growth promotion; Aspen; Pseudomonas; computational modeling; nitrogen; phosphorus; transportomics
OSTI Identifier:
1366508

Shinde, Shalaka, Cumming, Jonathan R., Collart, Frank R., Noirot, Philippe H., and Larsen, Peter E.. Pseudomonas fluorescens transportome is linked to strain-specific plant growth promotion in Aspen seedlings under nutrient stress. United States: N. p., Web. doi:10.3389/fpls.2017.00348.
Shinde, Shalaka, Cumming, Jonathan R., Collart, Frank R., Noirot, Philippe H., & Larsen, Peter E.. Pseudomonas fluorescens transportome is linked to strain-specific plant growth promotion in Aspen seedlings under nutrient stress. United States. doi:10.3389/fpls.2017.00348.
Shinde, Shalaka, Cumming, Jonathan R., Collart, Frank R., Noirot, Philippe H., and Larsen, Peter E.. 2017. "Pseudomonas fluorescens transportome is linked to strain-specific plant growth promotion in Aspen seedlings under nutrient stress". United States. doi:10.3389/fpls.2017.00348. https://www.osti.gov/servlets/purl/1366508.
@article{osti_1366508,
title = {Pseudomonas fluorescens transportome is linked to strain-specific plant growth promotion in Aspen seedlings under nutrient stress},
author = {Shinde, Shalaka and Cumming, Jonathan R. and Collart, Frank R. and Noirot, Philippe H. and Larsen, Peter E.},
abstractNote = {Diverse communities of bacteria colonize plant roots and the rhizosphere. Many of these rhizobacteria are symbionts and provide plant growth promotion (PGP) services, protecting the plant from biotic and abiotic stresses and increasing plant productivity by providing access to nutrients that would otherwise be unavailable to roots. In return, these symbiotic bacteria receive photosynthetically-derived carbon (C), in the form of sugars and organic acids, from plant root exudates. PGP activities have been characterized for a variety of forest tree species and are important in C cycling and sequestration in terrestrial ecosystems. The molecular mechanisms of these PGP activities, however, are less well-known. In a previous analysis of Pseudomonas genomes, we found that the bacterial transportome, the aggregate activity of a bacteria's transmembrane transporters, was most predictive for the ecological niche of Pseudomonads in the rhizosphere. Here, we used Populus tremuloides Michx. (trembling aspen) seedlings inoculated with one of three Pseudomonas fluorescens strains (Pf0-1, SBW25, and WH6) and one Pseudomonas protegens (Pf-5) as a laboratory model to further investigate the relationships between the predicted transportomic capacity of a bacterial strain and its observed PGP effects in laboratory cultures. Conditions of low nitrogen (N) or low phosphorus (P) availability and the corresponding replete media conditions were investigated. We measured phenotypic and biochemical parameters of P. tremuloides seedlings and correlated P fluorescens strain-specific transportomic capacities with P. tremuloides seedling phenotype to predict the strain and nutrient environment-specific transporter functions that lead to experimentally observed, strain, and media-specific PGP activities and the capacity to protect plants against nutrient stress. These predicted transportomic functions fall in three groups: (i) transport of compounds that modulate aspen seedling root architecture, (ii) transport of compounds that help to mobilize nutrients for aspen roots, and (iii) transporters that enable bacterial acquisition of C sources from seedling root exudates. Lastly, these predictions point to specific molecular mechanisms of PGP activities that can be directly tested through future, hypothesis-driven biological experiments.},
doi = {10.3389/fpls.2017.00348},
journal = {Frontiers in Plant Science},
number = ,
volume = 8,
place = {United States},
year = {2017},
month = {3}
}