Genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems
Abstract
It is generally accepted that diverse, poorly characterized microorganisms reside deep within Earth’s crust. One such lineage of deep subsurface-dwelling bacteria is an uncultivated member of theFirmicutesphylum that can dominate molecular surveys from both marine and continental rock fracture fluids, sometimes forming the sole member of a single-species microbiome. Here, we reconstructed a genome from basalt-hosted fluids of the deep subseafloor along the eastern Juan de Fuca Ridge flank and used a phylogenomic analysis to show that, despite vast differences in geographic origin and habitat, it forms a monophyletic clade with the terrestrial deep subsurface genome of “CandidatusDesulforudis audaxviator” MP104C. While a limited number of differences were observed between the marine genome of “CandidatusDesulfopertinax cowenii” modA32 and its terrestrial relative that may be of potential adaptive importance, here it is revealed that the two are remarkably similar thermophiles possessing the genetic capacity for motility, sporulation, hydrogenotrophy, chemoorganotrophy, dissimilatory sulfate reduction, and the ability to fix inorganic carbon via the Wood-Ljungdahl pathway for chemoautotrophic growth. Our results provide insights into the genetic repertoire within marine and terrestrial members of a bacterial lineage that is widespread in the global deep subsurface biosphere, and provides a natural means to investigate adaptations specific tomore »
- Authors:
-
- Univ. of Hawaii at Manoa, Honolulu, HI (United States); Univ. of Southern California, Los Angeles, CA (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
- USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
- Bigelow Lab. for Ocean Sciences, East Boothbay, ME (United States)
- Univ. of Hawaii at Manoa, Kaneohe, HI (United States)
- Publication Date:
- Research Org.:
- USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1358388
- Alternate Identifier(s):
- OSTI ID: 1379633
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PeerJ
- Additional Journal Information:
- Journal Volume: 5; Journal ID: ISSN 2167-8359
- Publisher:
- PeerJ Inc.
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; deep subsurface; microorganisms; Firmicutes; Juan de Fuca Ridge; chemoautotrophy; basement biosphere; sulfate reduction; sporulation; genomic; metagenomic
Citation Formats
Jungbluth, Sean P., Glavina del Rio, Tijana, Tringe, Susannah G., Stepanauskas, Ramunas, and Rappé, Michael S. Genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems. United States: N. p., 2017.
Web. doi:10.7717/peerj.3134.
Jungbluth, Sean P., Glavina del Rio, Tijana, Tringe, Susannah G., Stepanauskas, Ramunas, & Rappé, Michael S. Genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems. United States. https://doi.org/10.7717/peerj.3134
Jungbluth, Sean P., Glavina del Rio, Tijana, Tringe, Susannah G., Stepanauskas, Ramunas, and Rappé, Michael S. Thu .
"Genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems". United States. https://doi.org/10.7717/peerj.3134. https://www.osti.gov/servlets/purl/1358388.
@article{osti_1358388,
title = {Genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems},
author = {Jungbluth, Sean P. and Glavina del Rio, Tijana and Tringe, Susannah G. and Stepanauskas, Ramunas and Rappé, Michael S.},
abstractNote = {It is generally accepted that diverse, poorly characterized microorganisms reside deep within Earth’s crust. One such lineage of deep subsurface-dwelling bacteria is an uncultivated member of theFirmicutesphylum that can dominate molecular surveys from both marine and continental rock fracture fluids, sometimes forming the sole member of a single-species microbiome. Here, we reconstructed a genome from basalt-hosted fluids of the deep subseafloor along the eastern Juan de Fuca Ridge flank and used a phylogenomic analysis to show that, despite vast differences in geographic origin and habitat, it forms a monophyletic clade with the terrestrial deep subsurface genome of “CandidatusDesulforudis audaxviator” MP104C. While a limited number of differences were observed between the marine genome of “CandidatusDesulfopertinax cowenii” modA32 and its terrestrial relative that may be of potential adaptive importance, here it is revealed that the two are remarkably similar thermophiles possessing the genetic capacity for motility, sporulation, hydrogenotrophy, chemoorganotrophy, dissimilatory sulfate reduction, and the ability to fix inorganic carbon via the Wood-Ljungdahl pathway for chemoautotrophic growth. Our results provide insights into the genetic repertoire within marine and terrestrial members of a bacterial lineage that is widespread in the global deep subsurface biosphere, and provides a natural means to investigate adaptations specific to these two environments.},
doi = {10.7717/peerj.3134},
journal = {PeerJ},
number = ,
volume = 5,
place = {United States},
year = {Thu Apr 06 00:00:00 EDT 2017},
month = {Thu Apr 06 00:00:00 EDT 2017}
}
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