Assessment of Bacterial Inoculant Delivery Methods for Cereal Crops
Abstract
Despite growing evidence that plant growth-promoting bacteria can be used to improve crop vigor, a comparison of the different methods of delivery to determine which is optimal has not been published. An optimal inoculation method ensures that the inoculant colonizes the host plant so that its potential for plant growth-promotion is fully evaluated. The objective of this study was to compare the efficacy of three seed coating methods, seedling priming, and soil drench for delivering three bacterial inoculants to the sorghum rhizosphere and root endosphere. The methods were compared across multiple time points under axenic conditions and colonization efficiency was determined by quantitative polymerase chain reaction (qPCR). Two seed coating methods were also assessed in the field to test the reproducibility of the greenhouse results under non-sterile conditions. In the greenhouse seed coating methods were more successful in delivering the Gram-positive inoculant (Terrabacter sp.) while better colonization from the Gram-negative bacteria (Chitinophaga pinensis and Caulobacter rhizosphaerae) was observed with seedling priming and soil drench. This suggested that Gram-positive bacteria may be more suitable for the seed coating methods possibly because of their thick peptidoglycan cell wall. We also demonstrated that prolonged seed coating for 12 h could effectively enhance themore »
- Authors:
-
- University of Nebraska, Lincoln, NE (United States)
- Publication Date:
- Research Org.:
- Univ. of Nebraska, Lincoln, NE (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1904452
- Grant/Contract Number:
- SC0014395
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Microbiology
- Additional Journal Information:
- Journal Volume: 13; Journal ID: ISSN 1664-302X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; inoculation; plant growth promoting bacteria; rhizosphere; endosphere; Chitinophaga; Caulobacter; Terrabacter; sorghum
Citation Formats
Chai, Yen Ning, Futrell, Stephanie, and Schachtman, Daniel P. Assessment of Bacterial Inoculant Delivery Methods for Cereal Crops. United States: N. p., 2022.
Web. doi:10.3389/fmicb.2022.791110.
Chai, Yen Ning, Futrell, Stephanie, & Schachtman, Daniel P. Assessment of Bacterial Inoculant Delivery Methods for Cereal Crops. United States. https://doi.org/10.3389/fmicb.2022.791110
Chai, Yen Ning, Futrell, Stephanie, and Schachtman, Daniel P. Wed .
"Assessment of Bacterial Inoculant Delivery Methods for Cereal Crops". United States. https://doi.org/10.3389/fmicb.2022.791110. https://www.osti.gov/servlets/purl/1904452.
@article{osti_1904452,
title = {Assessment of Bacterial Inoculant Delivery Methods for Cereal Crops},
author = {Chai, Yen Ning and Futrell, Stephanie and Schachtman, Daniel P.},
abstractNote = {Despite growing evidence that plant growth-promoting bacteria can be used to improve crop vigor, a comparison of the different methods of delivery to determine which is optimal has not been published. An optimal inoculation method ensures that the inoculant colonizes the host plant so that its potential for plant growth-promotion is fully evaluated. The objective of this study was to compare the efficacy of three seed coating methods, seedling priming, and soil drench for delivering three bacterial inoculants to the sorghum rhizosphere and root endosphere. The methods were compared across multiple time points under axenic conditions and colonization efficiency was determined by quantitative polymerase chain reaction (qPCR). Two seed coating methods were also assessed in the field to test the reproducibility of the greenhouse results under non-sterile conditions. In the greenhouse seed coating methods were more successful in delivering the Gram-positive inoculant (Terrabacter sp.) while better colonization from the Gram-negative bacteria (Chitinophaga pinensis and Caulobacter rhizosphaerae) was observed with seedling priming and soil drench. This suggested that Gram-positive bacteria may be more suitable for the seed coating methods possibly because of their thick peptidoglycan cell wall. We also demonstrated that prolonged seed coating for 12 h could effectively enhance the colonization of C. pinensis, an endophytic bacterium, but not the rhizosphere colonizing C. rhizosphaerae. In the field only a small amount of inoculant was detected in the rhizosphere. This comparison demonstrates the importance of using the appropriate inoculation method for testing different types of bacteria for their plant growth-promotion potential.},
doi = {10.3389/fmicb.2022.791110},
journal = {Frontiers in Microbiology},
number = ,
volume = 13,
place = {United States},
year = {Wed Jan 26 00:00:00 EST 2022},
month = {Wed Jan 26 00:00:00 EST 2022}
}
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