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Title: Comparison of the plant growth-promotion performance of a consortium of Bacilli inoculated as endospores or as vegetative cells

Abstract

ABSTRACT The effect of three plant growth-promoting Bacillus strains inoculated either alone or as a consortium was tested on oat (Avena sativa) growth. The bioinoculants were applied as vegetative cells or endospores at low cell densities on the seeds and their effect was tested in sterile in vitro conditions, pot experiments, and a field trial. The in vitro seed germination assay showed that both individual bacterial inocula and bacterial consortia had positive effects on seed germination. Greenhouse pot experiments with sterile and non-sterile soil showed that consortia increased the total dry biomass of oat plants as compared to single strain inoculation and uninoculated controls. However, the positive impact on plant growth was less prominent when the bioinoculated strains had to compete with native soil microbes. Finally, the field experiment demonstrated that the consortium of vegetative cells was more efficient in promoting oat growth than the endospore consortium and the uninoculated control. Moreover, both consortia successfully colonized the roots and the rhizosphere of oat plants, without modifying the overall structure of the autochthonous soil microbial communities.

Authors:
 [1];  [1];  [1];  [1];  [1];  [2];  [1];  [1]
  1. Laboratory of Microbiology, University of Neuchâtel, 2000 Neuchâtel, Switzerland
  2. Laboratory of Microbiology, University of Neuchâtel, 2000 Neuchâtel, Switzerland, Vital-IT Group, Swiss Institute of Bioinformatics, 1015 Lausanne, Switzerland
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1570509
Resource Type:
Published Article
Journal Name:
FEMS Microbiology Ecology
Additional Journal Information:
Journal Name: FEMS Microbiology Ecology Journal Volume: 95 Journal Issue: 11; Journal ID: ISSN 0168-6496
Publisher:
Oxford University Press
Country of Publication:
Netherlands
Language:
English

Citation Formats

Hashmi, Isha, Paul, Christophe, Al-Dourobi, Andrej, Sandoz, Frederic, Deschamps, Priscilla, Junier, Thomas, Junier, Pilar, and Bindschedler, Saskia. Comparison of the plant growth-promotion performance of a consortium of Bacilli inoculated as endospores or as vegetative cells. Netherlands: N. p., 2019. Web. doi:10.1093/femsec/fiz147.
Hashmi, Isha, Paul, Christophe, Al-Dourobi, Andrej, Sandoz, Frederic, Deschamps, Priscilla, Junier, Thomas, Junier, Pilar, & Bindschedler, Saskia. Comparison of the plant growth-promotion performance of a consortium of Bacilli inoculated as endospores or as vegetative cells. Netherlands. doi:10.1093/femsec/fiz147.
Hashmi, Isha, Paul, Christophe, Al-Dourobi, Andrej, Sandoz, Frederic, Deschamps, Priscilla, Junier, Thomas, Junier, Pilar, and Bindschedler, Saskia. Thu . "Comparison of the plant growth-promotion performance of a consortium of Bacilli inoculated as endospores or as vegetative cells". Netherlands. doi:10.1093/femsec/fiz147.
@article{osti_1570509,
title = {Comparison of the plant growth-promotion performance of a consortium of Bacilli inoculated as endospores or as vegetative cells},
author = {Hashmi, Isha and Paul, Christophe and Al-Dourobi, Andrej and Sandoz, Frederic and Deschamps, Priscilla and Junier, Thomas and Junier, Pilar and Bindschedler, Saskia},
abstractNote = {ABSTRACT The effect of three plant growth-promoting Bacillus strains inoculated either alone or as a consortium was tested on oat (Avena sativa) growth. The bioinoculants were applied as vegetative cells or endospores at low cell densities on the seeds and their effect was tested in sterile in vitro conditions, pot experiments, and a field trial. The in vitro seed germination assay showed that both individual bacterial inocula and bacterial consortia had positive effects on seed germination. Greenhouse pot experiments with sterile and non-sterile soil showed that consortia increased the total dry biomass of oat plants as compared to single strain inoculation and uninoculated controls. However, the positive impact on plant growth was less prominent when the bioinoculated strains had to compete with native soil microbes. Finally, the field experiment demonstrated that the consortium of vegetative cells was more efficient in promoting oat growth than the endospore consortium and the uninoculated control. Moreover, both consortia successfully colonized the roots and the rhizosphere of oat plants, without modifying the overall structure of the autochthonous soil microbial communities.},
doi = {10.1093/femsec/fiz147},
journal = {FEMS Microbiology Ecology},
number = 11,
volume = 95,
place = {Netherlands},
year = {2019},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1093/femsec/fiz147

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Works referenced in this record:

Co-inoculation Effect of Rhizobia and Plant Growth Promoting Rhizobacteria on Common Bean Growth in a Low Phosphorus Soil
journal, February 2017

  • Korir, Hezekiah; Mungai, Nancy W.; Thuita, Moses
  • Frontiers in Plant Science, Vol. 08
  • DOI: 10.3389/fpls.2017.00141

Distinct Microbial Communities within the Endosphere and Rhizosphere of Populus deltoides Roots across Contrasting Soil Types
journal, July 2011

  • Gottel, Neil R.; Castro, Hector F.; Kerley, Marilyn
  • Applied and Environmental Microbiology, Vol. 77, Issue 17
  • DOI: 10.1128/AEM.05255-11

Efficiency of two inoculation methods of Pseudomonas putida on growth and yield of tomato plants
journal, January 2017

  • Hernández-Montiel, Luis G.; Chiquito Contreras, César J.; Murillo Amador, Bernardo
  • Journal of soil science and plant nutrition, Vol. 17, Issue 4
  • DOI: 10.4067/S0718-95162017000400012

Effect of inoculation of Bacillus licheniformis on tomato and pepper
journal, May 2004

  • García, Jose Antonio Lucas; Probanza, Agustín; Ramos, Beatriz
  • Agronomie, Vol. 24, Issue 4
  • DOI: 10.1051/agro:2004020

Appraisal of the crop-rotation effect of rhizobial inoculation on potato cropping systems in relation to soil bacterial communities
journal, November 2012


Effect of plant growth promoting rhizobacterial (PGPR) inoculation on growth in tomato (Solanum Lycopersicum L.) and characterization for direct PGP abilities in Morocco
journal, January 2017

  • M., Moustaine; R., Elkahkahi; A., Benbouazza
  • International Journal of Environment, Agriculture and Biotechnology, Vol. 2, Issue 2
  • DOI: 10.22161/ijeab/2.2.5

Specificity in Arabidopsis thaliana recruitment of root fungal communities from soil and rhizosphere
journal, April 2018


Identification of common molecular subsequences
journal, March 1981


Gibberellins producing Bacillus methylotrophicus KE2 supports plant growth and enhances nutritional metabolites and food values of lettuce
journal, December 2016


Stage 0 sporulation gene A as a molecular marker to study diversity of endospore-forming Firmicutes: Culture-independent study of endospore-forming Firmicutes
journal, September 2013

  • Wunderlin, Tina; Junier, Thomas; Roussel-Delif, Ludovic
  • Environmental Microbiology Reports, Vol. 5, Issue 6
  • DOI: 10.1111/1758-2229.12094

Effect of inoculation with a thermotolerant plant growth promoting Pseudomonas putida strain AKMP7 on growth of wheat ( Triticum spp.) under heat stress
journal, December 2011

  • Ali, Shaik. Zulfikar; Sandhya, Vardharajula; Grover, Minakshi
  • Journal of Plant Interactions, Vol. 6, Issue 4
  • DOI: 10.1080/17429145.2010.545147

Living inside plants: bacterial endophytes
journal, August 2011


Simplified and representative bacterial community of maize roots
journal, March 2017

  • Niu, Ben; Paulson, Joseph Nathaniel; Zheng, Xiaoqi
  • Proceedings of the National Academy of Sciences, Vol. 114, Issue 12
  • DOI: 10.1073/pnas.1616148114

Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform
journal, June 2013

  • Kozich, James J.; Westcott, Sarah L.; Baxter, Nielson T.
  • Applied and Environmental Microbiology, Vol. 79, Issue 17
  • DOI: 10.1128/AEM.01043-13

Alleviation of drought stress in the common bean (Phaseolus vulgaris L.) by co-inoculation with Paenibacillus polymyxa and Rhizobium tropici
journal, September 2008

  • Figueiredo, Márcia V. B.; Burity, Hélio A.; Martínez, Cosme R.
  • Applied Soil Ecology, Vol. 40, Issue 1
  • DOI: 10.1016/j.apsoil.2008.04.005

The competition between Rhizobium leguminosarum bv. viciae strains progresses until late stages of symbiosis
journal, August 2010


Heavy Metal Polluted Soils: Effect on Plants and Bioremediation Methods
journal, January 2014

  • Chibuike, G. U.; Obiora, S. C.
  • Applied and Environmental Soil Science, Vol. 2014
  • DOI: 10.1155/2014/752708

Sampling, defining, characterising and modeling the rhizosphere—the soil science tool box
journal, October 2008


Microbial Inoculants and Their Impact on Soil Microbial Communities: A Review
journal, January 2013

  • Trabelsi, Darine; Mhamdi, Ridha
  • BioMed Research International, Vol. 2013
  • DOI: 10.1155/2013/863240

Resistance, resilience, and redundancy in microbial communities
journal, August 2008

  • Allison, S. D.; Martiny, J. B. H.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue Supplement 1
  • DOI: 10.1073/pnas.0801925105

Next generation of microbial inoculants for agriculture and bioremediation
journal, October 2016

  • Baez-Rogelio, Antonino; Morales-García, Yolanda Elizabeth; Quintero-Hernández, Verónica
  • Microbial Biotechnology, Vol. 10, Issue 1
  • DOI: 10.1111/1751-7915.12448

A new primer for discrimination of arbuscular mycorrhizal fungi with polymerase chain reaction-denature gradient gel electrophoresis
journal, June 2005


The use of a simple flow cytometry method for rapid detection of spores in probiotic Bacillus licheniformis-containing tablets
journal, February 2017

  • Zheng, Xiao-Ling; Xiong, Zhi-Qiang; Wu, Jie-Qun
  • Food Science and Biotechnology, Vol. 26, Issue 1
  • DOI: 10.1007/s10068-017-0022-5

Universal chemical assay for the detection and determination of siderophores
journal, January 1987


phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data
journal, April 2013


Bioprospecting of aerobic endospore-forming bacteria with biotechnological potential for growth promotion of banana plants
journal, November 2016


Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture
journal, December 2011

  • Bhattacharyya, P. N.; Jha, D. K.
  • World Journal of Microbiology and Biotechnology, Vol. 28, Issue 4
  • DOI: 10.1007/s11274-011-0979-9

Effects of two different application methods of Burkholderia ambifaria MCI 7 on plant growth and rhizospheric bacterial diversity
journal, April 2002


Adapting Collaborative Software Development Techniques to Structural Engineering
journal, September 2015

  • Sempolinski, Peter; Thain, Douglas; Wei, Zhigang
  • Computing in Science & Engineering, Vol. 17, Issue 5
  • DOI: 10.1109/MCSE.2015.88

The SILVA ribosomal RNA gene database project: improved data processing and web-based tools
journal, November 2012

  • Quast, Christian; Pruesse, Elmar; Yilmaz, Pelin
  • Nucleic Acids Research, Vol. 41, Issue D1
  • DOI: 10.1093/nar/gks1219

Plant growth-promoting bacteria as inoculants in agricultural soils
journal, November 2015

  • Souza, Rocheli de; Ambrosini, Adriana; Passaglia, Luciane M. P.
  • Genetics and Molecular Biology, Vol. 38, Issue 4
  • DOI: 10.1590/S1415-475738420150053

Evaluation of the Effectiveness of Tomato-Associated Rhizobacteria Applied Singly or as Three-Strain Consortium for Biosuppression of Sclerotinia Stem Rot in Tomato
journal, January 2016

  • Abdeljalil, Nada Ouhaibi Ben; Renault, David
  • Journal of Microbial & Biochemical Technology, Vol. 8, Issue 4
  • DOI: 10.4172/1948-5948.1000302

Ability of bacterium Bacillus subtilis to produce cytokinins and to influence the growth and endogenous hormone content of lettuce plants
journal, May 2005


The bacterial species definition in the genomic era
journal, October 2006

  • Konstantinidis, Konstantinos T.; Ramette, Alban; Tiedje, James M.
  • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 361, Issue 1475
  • DOI: 10.1098/rstb.2006.1920

Plant Growth Promotion and Suppression of Bacterial Leaf Blight in Rice by Inoculated Bacteria
journal, August 2016


Effect of Different Plant Growth Promoting Rhizobacteria on Maize Seed Germination and Seedling Development
journal, January 2013

  • Noumavo, Pacôme A.; Kochoni, Eméric; Didagbé, Yédéou O.
  • American Journal of Plant Sciences, Vol. 04, Issue 05
  • DOI: 10.4236/ajps.2013.45125

In vitro antagonistic activity, plant growth promoting traits and phylogenetic affiliation of rhizobacteria associated with wild plants grown in arid soil
journal, December 2014

  • El-Sayed, Wael S.; Akhkha, Abdellah; El-Naggar, Moustafa Y.
  • Frontiers in Microbiology, Vol. 5
  • DOI: 10.3389/fmicb.2014.00651

Evaluation of six primer pairs targeting the nuclear rRNA operon for characterization of arbuscular mycorrhizal fungal (AMF) communities using 454 pyrosequencing
journal, November 2014


Bacillus spp. inoculation improves photosystem II efficiency and enhances photosynthesis in pepper plants
journal, January 2016

  • Samaniego-Gámez, Blancka Yesenia; Garruña, René; Tun-Suárez, José M.
  • Chilean journal of agricultural research, Vol. 76, Issue 4
  • DOI: 10.4067/S0718-58392016000400003

Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
journal, October 2009

  • Schloss, P. D.; Westcott, S. L.; Ryabin, T.
  • Applied and Environmental Microbiology, Vol. 75, Issue 23, p. 7537-7541
  • DOI: 10.1128/AEM.01541-09

Microbial inoculation of seed for improved crop performance: issues and opportunities
journal, May 2016


Soil Inoculation with Bacillus spp. Modifies Root Endophytic Bacterial Diversity, Evenness, and Community Composition in a Context-Specific Manner
journal, March 2018


OptiClust, an Improved Method for Assigning Amplicon-Based Sequence Data to Operational Taxonomic Units
journal, March 2017


Nutrient-induced spore germination of a Bacillus amyloliquefaciens biocontrol agent on wheat spikes
journal, March 2014

  • Crane, J. M.; Frodyma, M. E.; Bergstrom, G. C.
  • Journal of Applied Microbiology, Vol. 116, Issue 6
  • DOI: 10.1111/jam.12480

A new PGPR co-inoculated with Bradyrhizobium japonicum enhances soybean nodulation
journal, July 2014


Bacillus: A Biological Tool for Crop Improvement through Bio-Molecular Changes in Adverse Environments
journal, September 2017

  • Radhakrishnan, Ramalingam; Hashem, Abeer; Abd_Allah, Elsayed F.
  • Frontiers in Physiology, Vol. 8
  • DOI: 10.3389/fphys.2017.00667

Use of Agrochemical Fertilizers and Their Impact on Soil, Water and Human Health in the Khamargao Village of Mymensingh District, Bangladesh
journal, February 2005

  • ., Md. Muhibbullah; ., Salma Momotaz; ., A. T. Chowdhury
  • Journal of Agronomy, Vol. 4, Issue 2
  • DOI: 10.3923/ja.2005.109.115

Influence of introduced potential biocontrol agents on maize seedling growth and bacterial community structure in the rhizosphere
journal, November 2004


Effects of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient contents in organically growing raspberry
journal, December 2006


Transitions in bacterial communities along the 2000 km salinity gradient of the Baltic Sea
journal, April 2011

  • Herlemann, Daniel PR; Labrenz, Matthias; Jürgens, Klaus
  • The ISME Journal, Vol. 5, Issue 10
  • DOI: 10.1038/ismej.2011.41

New Culture Medium Containing Ionic Concentrations of Nutrients Similar to Concentrations Found in the Soil Solution †
journal, January 1991


Rapid In Situ Assay for Indoleacetic Acid Production by Bacteria Immobilized on a Nitrocellulose Membrane
journal, January 1991

  • Bric, John M.; Bostock, Richard M.; Silverstone, Sara E.
  • Applied and Environmental Microbiology, Vol. 57, Issue 2
  • DOI: 10.1128/AEM.57.2.535-538.1991

Chemically Defined, Synthetic Media for Sporulation and for Germination and Growth of Bacillus Subtilis
journal, January 1964


Studies on the Proteolytic Bacteria of Milk
journal, January 1931


Survival of Bacillus thuringiensis Spores in Soil †
journal, January 1985