Performance of abiotic stress-inducible synthetic promoters in genetically engineered hybrid poplar (Populus tremula × Populus alba)

Yang, Yongil; Shao, Yuanhua; Chaffin, Timothy A.; Lee, Jun Hyung; Poindexter, Magen R.; Ahkami, Amir H.; Blumwald, Eduardo; Stewart, Jr, C. Neal

doi:10.3389/fpls.2022.1011939

Title: Performance of abiotic stress-inducible synthetic promoters in genetically engineered hybrid poplar (Populus tremula × Populus alba)

Abstract

Abiotic stresses can cause significant damage to plants. For sustainable bioenergy crop production, it is critical to generate resistant crops to such stress. Engineering promoters to control the precise expression of stress resistance genes is a very effective way to address the problem. Here we developed stably transformed Populus tremula × Populus alba hybrid poplar (INRA 717-1B4) containing one-of-six s ynthetic d rought stress-inducible promoters (SDs; SD9-1, SD9-2, SD9-3, SD13-1, SD18-1, and SD18-3) identified previously by transient transformation assays. We screened green fluorescent protein (GFP) induction in poplar under osmotic stress conditions. Of six transgenic lines containing synthetic promoter, three lines (SD18-1, 9-2, and 9-3) had significant GFP expression in both salt and osmotic stress treatments. Each synthetic promoter employed heptamerized repeats of specific and short cis -regulatory elements (7 repeats of 7-8 bases). To verify whether the repeats of longer sequences can improve osmotic stress responsiveness, a transgenic poplar containing the synthetic promoter of the heptamerized entire SD9 motif (20 bases, containing all partial SD9 motifs) was generated and measured for GFP induction under osmotic stress. The heptamerized entire SD9 motif did not result in higher GFP expression than the shorter promoters consisting of heptamerized SD9-1, 9-2, and 9-3more »« less

Authors:: Yang, Yongil; Shao, Yuanhua; Chaffin, Timothy A.; Lee, Jun Hyung; Poindexter, Magen R.; Ahkami, Amir H.; Blumwald, Eduardo; Stewart, Jr, C. Neal

Publication Date:: Tue Oct 18 00:00:00 EDT 2022

Research Org.:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1893536

Alternate Identifier(s):: OSTI ID: 1900520

Report Number(s):: PNNL-SA-174829
Journal ID: ISSN 1664-462X; 1011939

Grant/Contract Number:: AC05-76RL01830; SC0018347

Resource Type:: Published Article

Journal Name:: Frontiers in Plant Science

Additional Journal Information:: Journal Name: Frontiers in Plant Science Journal Volume: 13; Journal ID: ISSN 1664-462X

Publisher:: Frontiers Media SA

Country of Publication:: Switzerland

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; synthetic biology; synthetic promoter; poplar; abiotic stress; cis-regulatory elements; bioenergy

Citation Formats


                    Yang, Yongil, Shao, Yuanhua, Chaffin, Timothy A., Lee, Jun Hyung, Poindexter, Magen R., Ahkami, Amir H., Blumwald, Eduardo, and Stewart, Jr, C. Neal. Performance of abiotic stress-inducible synthetic promoters in genetically engineered hybrid poplar (Populus tremula × Populus alba).  Switzerland: N. p., 2022. 
Web.  doi:10.3389/fpls.2022.1011939.

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                    Yang, Yongil, Shao, Yuanhua, Chaffin, Timothy A., Lee, Jun Hyung, Poindexter, Magen R., Ahkami, Amir H., Blumwald, Eduardo, & Stewart, Jr, C. Neal. Performance of abiotic stress-inducible synthetic promoters in genetically engineered hybrid poplar (Populus tremula × Populus alba).  Switzerland.  https://doi.org/10.3389/fpls.2022.1011939

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                    Yang, Yongil, Shao, Yuanhua, Chaffin, Timothy A., Lee, Jun Hyung, Poindexter, Magen R., Ahkami, Amir H., Blumwald, Eduardo, and Stewart, Jr, C. Neal. Tue .  
"Performance of abiotic stress-inducible synthetic promoters in genetically engineered hybrid poplar (Populus tremula × Populus alba)".  Switzerland.  https://doi.org/10.3389/fpls.2022.1011939.

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@article{osti_1893536,

  title        = {Performance of abiotic stress-inducible synthetic promoters in genetically engineered hybrid poplar (Populus tremula × Populus alba)},

  author       = {Yang, Yongil and Shao, Yuanhua and Chaffin, Timothy A. and Lee, Jun Hyung and Poindexter, Magen R. and Ahkami, Amir H. and Blumwald, Eduardo and Stewart, Jr, C. Neal},

  abstractNote = {Abiotic stresses can cause significant damage to plants. For sustainable bioenergy crop production, it is critical to generate resistant crops to such stress. Engineering promoters to control the precise expression of stress resistance genes is a very effective way to address the problem. Here we developed stably transformed Populus tremula × Populus alba hybrid poplar (INRA 717-1B4) containing one-of-six s ynthetic d rought stress-inducible promoters (SDs; SD9-1, SD9-2, SD9-3, SD13-1, SD18-1, and SD18-3) identified previously by transient transformation assays. We screened green fluorescent protein (GFP) induction in poplar under osmotic stress conditions. Of six transgenic lines containing synthetic promoter, three lines (SD18-1, 9-2, and 9-3) had significant GFP expression in both salt and osmotic stress treatments. Each synthetic promoter employed heptamerized repeats of specific and short cis -regulatory elements (7 repeats of 7-8 bases). To verify whether the repeats of longer sequences can improve osmotic stress responsiveness, a transgenic poplar containing the synthetic promoter of the heptamerized entire SD9 motif (20 bases, containing all partial SD9 motifs) was generated and measured for GFP induction under osmotic stress. The heptamerized entire SD9 motif did not result in higher GFP expression than the shorter promoters consisting of heptamerized SD9-1, 9-2, and 9-3 (partial SD9) motifs. This result indicates that shorter synthetic promoters (~50 bp) can be used for versatile control of gene expression in transgenic poplar. These synthetic promoters will be useful tools to engineer stress-resilient bioenergy tree crops in the future.},

  doi          = {10.3389/fpls.2022.1011939},

  journal      = {Frontiers in Plant Science},

  number       = ,

  volume       = 13,

  place        = {Switzerland},

  year         = {Tue Oct 18 00:00:00 EDT 2022},

  month        = {Tue Oct 18 00:00:00 EDT 2022}

}

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Title: Performance of abiotic stress-inducible synthetic promoters in genetically engineered hybrid poplar (Populus tremula × Populus alba)

Abstract

Citation Formats

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Core promoter sequence in yeast is a major determinant of expression level journal, May 2015

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Distinct Role of Core Promoter Architecture in Regulation of Light-Mediated Responses in Plant Genes journal, April 2014

Vessel-Specific Reintroduction of CINNAMOYL-COA REDUCTASE1 (CCR1) in Dwarfed ccr1 Mutants Restores Vessel and Xylary Fiber Integrity and Increases Biomass journal, November 2017

Translation regulation in plants: an interesting past, an exciting present and a promising future journal, March 2017

Building the Plant SynBio Toolbox through Combinatorial Analysis of DNA Regulatory Elements journal, July 2022

Strength comparison between cold-inducible promoters of Arabidopsis cor15a and cor15b genes in potato and tobacco journal, October 2013

Multiple Classes of Transcription Factors Regulate the Expression of VASCULAR-RELATED NAC-DOMAIN7, a Master Switch of Xylem Vessel Differentiation journal, September 2014

The Q-System as a Synthetic Transcriptional Regulator in Plants journal, March 2020

Identification of Plant Enhancers and Their Constituent Elements by STARR-seq in Tobacco Leaves journal, May 2020

The Expression of CARK1 or RCAR11 Driven by Synthetic Promoters Increases Drought Tolerance in Arabidopsis thaliana journal, July 2018

Transcript elongation factors: shaping transcriptomes after transcript initiation journal, November 2014

Determinants of enhancer and promoter activities of regulatory elements journal, October 2019

Plant synthetic biology journal, May 2015

Imaging of multiple fluorescent proteins in canopies enables synthetic biology in plants journal, December 2020

Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants journal, November 2006

Synthetic promoter designs enabled by a comprehensive analysis of plant core promoters journal, June 2021

Rational design and testing of abiotic stress‐inducible synthetic promoters from poplar cis ‐regulatory elements journal, February 2021

An atlas of active enhancers across human cell types and tissues journal, March 2014

Design of orthogonal regulatory systems for modulating gene expression in plants journal, May 2020

Specific Bacterial Pathogen Phytosensing Is Enabled by a Synthetic Promoter-Transcription Factor System in Potato journal, April 2022

Innovation in plant-based transient protein expression for infectious disease prevention and preparedness journal, February 2020

Synthetic promoters in planta journal, August 2015

Genome-wide mapping of autonomous promoter activity in human cells journal, December 2016

Plant synthetic promoters and transcription factors journal, February 2016

Genome sequence and genetic transformation of a widely distributed and cultivated poplar journal, October 2018

Abiotic stress responses in plants journal, September 2021

Model-driven generation of artificial yeast promoters journal, April 2020

Systematic interrogation of human promoters journal, January 2019

Systematic Localization of Common Disease-Associated Variation in Regulatory DNA journal, September 2012

Gene activation by metazoan enhancers: Diverse mechanisms stimulate distinct steps of transcription
journal, July 2016

Plant abiotic stress: a prospective strategy of exploiting promoters as alternative to overcome the escalating burden
journal, August 2015

The regulatory landscape of Arabidopsis thaliana roots at single-cell resolution
journal, June 2021

Cold-induced silencing by long antisense transcripts of an Arabidopsis Polycomb target
journal, December 2009

Eukaryotic core promoters and the functional basis of transcription initiation
journal, June 2018

Differentiation of core promoter architecture between plants and mammals revealed by LDSS analysis
journal, September 2007

Genetic Transformation of Populus trichocarpa Genotype Nisqually-1: A Functional Genomic Tool for Woody Plants
journal, November 2006

Deciphering eukaryotic gene-regulatory logic with 100 million random promoters
journal, December 2019

Single-cell transcriptome atlas and chromatin accessibility landscape reveal differentiation trajectories in the rice root
journal, April 2021

Plant synthetic biology innovations for biofuels and bioproducts
journal, October 2022

A gene-editing/complementation strategy for tissue-specific lignin reduction while preserving biomass yield
journal, September 2021

Plant-produced biopharmaceuticals: A case of technical developments driving clinical deployment
journal, September 2016

Transgenic tobacco plants overexpressing the heterologous lea gene Rab16A from rice during high salt and water deficit display enhanced tolerance to salinity stress
journal, June 2007

Core promoter sequence in yeast is a major determinant of expression level
journal, May 2015

NAC-MYB-based transcriptional regulation of secondary cell wall biosynthesis in land plants
journal, May 2015

Transgenic Poplar Designed for Biofuels
journal, September 2020

Abiotic Stresses: General Defenses of Land Plants and Chances for Engineering Multistress Tolerance
journal, December 2018

Heat Stress-Mediated Constraints in Maize (Zea mays) Production: Challenges and Solutions
journal, April 2022

Genome-wide assessment of sequence-intrinsic enhancer responsiveness at single-base-pair resolution
journal, December 2016

NIH Image to ImageJ: 25 years of image analysis
journal, June 2012

DNA assembly standards: Setting the low-level programming code for plant biotechnology
journal, August 2018

Paired-End Analysis of Transcription Start Sites in Arabidopsis Reveals Plant-Specific Promoter Signatures
journal, July 2014

A cis-regulatory atlas in maize at single-cell resolution
journal, May 2021

Transcriptional enhancers: from properties to genome-wide predictions
journal, March 2014

A Fruitful Decade Using Synthetic Promoters in the Improvement of Transgenic Plants
journal, November 2019

Metazoan promoters: emerging characteristics and insights into transcriptional regulation
journal, March 2012

Rational design of minimal synthetic promoters for plants
journal, August 2020

The Genome of Black Cottonwood, Populus trichocarpa (Torr. & Gray)
journal, September 2006

Gene silencing in plants: A diversity of pathways
journal, December 2013

Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor
journal, March 1999

Synthetic Switches and Regulatory Circuits in Plants
journal, January 2019

Distinct Role of Core Promoter Architecture in Regulation of Light-Mediated Responses in Plant Genes
journal, April 2014

Vessel-Specific Reintroduction of CINNAMOYL-COA REDUCTASE1 (CCR1) in Dwarfed ccr1 Mutants Restores Vessel and Xylary Fiber Integrity and Increases Biomass
journal, November 2017

Translation regulation in plants: an interesting past, an exciting present and a promising future
journal, March 2017

Building the Plant SynBio Toolbox through Combinatorial Analysis of DNA Regulatory Elements
journal, July 2022

Strength comparison between cold-inducible promoters of Arabidopsis cor15a and cor15b genes in potato and tobacco
journal, October 2013

Multiple Classes of Transcription Factors Regulate the Expression of VASCULAR-RELATED NAC-DOMAIN7, a Master Switch of Xylem Vessel Differentiation
journal, September 2014

The Q-System as a Synthetic Transcriptional Regulator in Plants
journal, March 2020

Identification of Plant Enhancers and Their Constituent Elements by STARR-seq in Tobacco Leaves
journal, May 2020

The Expression of CARK1 or RCAR11 Driven by Synthetic Promoters Increases Drought Tolerance in Arabidopsis thaliana
journal, July 2018

Transcript elongation factors: shaping transcriptomes after transcript initiation
journal, November 2014

Determinants of enhancer and promoter activities of regulatory elements
journal, October 2019

Plant synthetic biology
journal, May 2015

Imaging of multiple fluorescent proteins in canopies enables synthetic biology in plants
journal, December 2020

Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
journal, November 2006

Synthetic promoter designs enabled by a comprehensive analysis of plant core promoters
journal, June 2021

Rational design and testing of abiotic stress‐inducible synthetic promoters from poplar cis ‐regulatory elements
journal, February 2021

An atlas of active enhancers across human cell types and tissues
journal, March 2014

Design of orthogonal regulatory systems for modulating gene expression in plants
journal, May 2020

Specific Bacterial Pathogen Phytosensing Is Enabled by a Synthetic Promoter-Transcription Factor System in Potato
journal, April 2022

Innovation in plant-based transient protein expression for infectious disease prevention and preparedness
journal, February 2020

Synthetic promoters in planta
journal, August 2015

Genome-wide mapping of autonomous promoter activity in human cells
journal, December 2016

Plant synthetic promoters and transcription factors
journal, February 2016

Genome sequence and genetic transformation of a widely distributed and cultivated poplar
journal, October 2018

Abiotic stress responses in plants
journal, September 2021

Model-driven generation of artificial yeast promoters
journal, April 2020

Systematic interrogation of human promoters
journal, January 2019

Systematic Localization of Common Disease-Associated Variation in Regulatory DNA
journal, September 2012