A bottom-up perspective on photodynamics and photoprotection in light-harvesting complexes using anti-Brownian trapping
Abstract
Single-molecule fluorescence spectroscopy allows direct, real-time observation of dynamic photophysical changes in light harvesting complexes. The Anti-Brownian ELectrokinetic (ABEL) trap is one such single-molecule method with useful advantages. This approach is particularly well-suited to make detailed spectroscopic measurements of pigment-protein complexes in solution phase because it enables extended-duration single-molecule observation by counteracting Brownian motion. This Perspective summarizes recent contributions by the authors and others that have utilized the unique capabilities of the ABEL trap to advance our understanding of phycobiliproteins and the phycobilisome complex, the primary light-harvesting apparatus of cyanobacteria. Monitoring the rich spectroscopic data from these measurements, which include brightness, fluorescence lifetime, polarization, and emission spectra, among other measurable parameters, has provided direct characterization of pigments and energy transfer pathways in the phycobilisome, spanning scales from single pigments and monomeric phycobiliproteins to higher order oligomers and protein-protein interactions of the phycobilisome complex. Importantly, new photophysical states and photodynamics were observed to modulate the flow of energy through the phycobilisome and suggest a previously unknown complexity in phycobilisome light harvesting and energy transport with a possible link to photoadaptive or photoprotective functions in cyanobacteria. Here, beyond deepening our collective understanding of natural light-harvesting systems, these and future discoveries may servemore »
- Authors:
-
[1];
[1];
[2];
[1]
- Stanford University, CA (United States)
- Stanford University, CA (United States); SLAC National Accelerator Lab., Menlo Park, CA (United States)
- Publication Date:
- Research Org.:
- SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States); Stanford Univ., CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division; USDOE
- OSTI Identifier:
- 1882690
- Alternate Identifier(s):
- OSTI ID: 1843432; OSTI ID: 1845343
- Grant/Contract Number:
- AC02-76SF00515; FG02-07ER15892
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Chemical Physics
- Additional Journal Information:
- Journal Volume: 156; Journal Issue: 7; Journal ID: ISSN 0021-9606
- Publisher:
- American Institute of Physics (AIP)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 14 SOLAR ENERGY; 77 NANOSCIENCE AND NANOTECHNOLOGY
Citation Formats
Squires, Allison H., Wang, Quan, Dahlberg, Peter D., and Moerner, W. E. A bottom-up perspective on photodynamics and photoprotection in light-harvesting complexes using anti-Brownian trapping. United States: N. p., 2022.
Web. doi:10.1063/5.0079042.
Squires, Allison H., Wang, Quan, Dahlberg, Peter D., & Moerner, W. E. A bottom-up perspective on photodynamics and photoprotection in light-harvesting complexes using anti-Brownian trapping. United States. https://doi.org/10.1063/5.0079042
Squires, Allison H., Wang, Quan, Dahlberg, Peter D., and Moerner, W. E. Wed .
"A bottom-up perspective on photodynamics and photoprotection in light-harvesting complexes using anti-Brownian trapping". United States. https://doi.org/10.1063/5.0079042. https://www.osti.gov/servlets/purl/1882690.
@article{osti_1882690,
title = {A bottom-up perspective on photodynamics and photoprotection in light-harvesting complexes using anti-Brownian trapping},
author = {Squires, Allison H. and Wang, Quan and Dahlberg, Peter D. and Moerner, W. E.},
abstractNote = {Single-molecule fluorescence spectroscopy allows direct, real-time observation of dynamic photophysical changes in light harvesting complexes. The Anti-Brownian ELectrokinetic (ABEL) trap is one such single-molecule method with useful advantages. This approach is particularly well-suited to make detailed spectroscopic measurements of pigment-protein complexes in solution phase because it enables extended-duration single-molecule observation by counteracting Brownian motion. This Perspective summarizes recent contributions by the authors and others that have utilized the unique capabilities of the ABEL trap to advance our understanding of phycobiliproteins and the phycobilisome complex, the primary light-harvesting apparatus of cyanobacteria. Monitoring the rich spectroscopic data from these measurements, which include brightness, fluorescence lifetime, polarization, and emission spectra, among other measurable parameters, has provided direct characterization of pigments and energy transfer pathways in the phycobilisome, spanning scales from single pigments and monomeric phycobiliproteins to higher order oligomers and protein-protein interactions of the phycobilisome complex. Importantly, new photophysical states and photodynamics were observed to modulate the flow of energy through the phycobilisome and suggest a previously unknown complexity in phycobilisome light harvesting and energy transport with a possible link to photoadaptive or photoprotective functions in cyanobacteria. Here, beyond deepening our collective understanding of natural light-harvesting systems, these and future discoveries may serve as inspiration for engineering improved artificial light-harvesting technologies.},
doi = {10.1063/5.0079042},
journal = {Journal of Chemical Physics},
number = 7,
volume = 156,
place = {United States},
year = {Wed Feb 16 00:00:00 EST 2022},
month = {Wed Feb 16 00:00:00 EST 2022}
}
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