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Title: Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein

Abstract

The Orange Carotenoid Protein (OCP) is a cytosolic photosensor that is responsible for nonphotochemical quenching (NPQ) of the light-harvesting process in most cyanobacteria. Upon photoactivation by blue-green light, OCP binds to the phycobilisome antenna complex, providing an excitonic trap to thermally dissipate excess energy. At present, both the binding site and NPQ mechanism of OCP are unknown. Using an Anti-Brownian ELectrokinetic (ABEL) trap, we isolate single phycobilisomes in free solution, both in the presence and absence of activated OCP, to directly determine the photophysics and heterogeneity of OCPquenched phycobilisomes. Surprisingly, we observe two distinct OCP-quenched states, with lifetimes 0.09 ns (6% of unquenched brightness) and 0.21 ns (11% brightness). Photon-byphoton Monte Carlo simulations of exciton transfer through the phycobilisome suggest that the observed quenched states are kinetically consistent with either two or one bound OCPs, respectively, underscoring an additional mechanism for excitation control in this key photosynthetic unit.

Authors:
ORCiD logo [1];  [1]; ORCiD logo [2];  [2]; ORCiD logo [2]; ORCiD logo [1]
+ Show Author Affiliations
  1. Stanford Univ., CA (United States). Dept. of Chemistry
  2. Washington Univ., St. Louis, MO (United States). Dept. of Biology and Chemistry
Publication Date:
Research Org.:
Stanford Univ., CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1501915
Grant/Contract Number:  
FG02-07ER15892
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Squires, Allison H., Dahlberg, Peter D., Liu, Haijun, Magdaong, Nikki Cecil M., Blankenship, Robert E., and Moerner, W. E. Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein. United States: N. p., 2019. Web. doi:10.1038/s41467-019-09084-2.
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Squires, Allison H., Dahlberg, Peter D., Liu, Haijun, Magdaong, Nikki Cecil M., Blankenship, Robert E., & Moerner, W. E. Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein. United States. https://doi.org/10.1038/s41467-019-09084-2
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Squires, Allison H., Dahlberg, Peter D., Liu, Haijun, Magdaong, Nikki Cecil M., Blankenship, Robert E., and Moerner, W. E. Tue . "Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein". United States. https://doi.org/10.1038/s41467-019-09084-2. https://www.osti.gov/servlets/purl/1501915.
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@article{osti_1501915,
title = {Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein},
author = {Squires, Allison H. and Dahlberg, Peter D. and Liu, Haijun and Magdaong, Nikki Cecil M. and Blankenship, Robert E. and Moerner, W. E.},
abstractNote = {The Orange Carotenoid Protein (OCP) is a cytosolic photosensor that is responsible for nonphotochemical quenching (NPQ) of the light-harvesting process in most cyanobacteria. Upon photoactivation by blue-green light, OCP binds to the phycobilisome antenna complex, providing an excitonic trap to thermally dissipate excess energy. At present, both the binding site and NPQ mechanism of OCP are unknown. Using an Anti-Brownian ELectrokinetic (ABEL) trap, we isolate single phycobilisomes in free solution, both in the presence and absence of activated OCP, to directly determine the photophysics and heterogeneity of OCPquenched phycobilisomes. Surprisingly, we observe two distinct OCP-quenched states, with lifetimes 0.09 ns (6% of unquenched brightness) and 0.21 ns (11% brightness). Photon-byphoton Monte Carlo simulations of exciton transfer through the phycobilisome suggest that the observed quenched states are kinetically consistent with either two or one bound OCPs, respectively, underscoring an additional mechanism for excitation control in this key photosynthetic unit.},
doi = {10.1038/s41467-019-09084-2},
journal = {Nature Communications},
number = 1,
volume = 10,
place = {United States},
year = {Tue Mar 12 00:00:00 EDT 2019},
month = {Tue Mar 12 00:00:00 EDT 2019}
}
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Journal Article:
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https://doi.org/10.1038/s41467-019-09084-2
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Figures / Tables:

Fig. 1 Fig. 1 : OCP quenches fluorescence in the phycobilisome. a Structure of OCP in inactive orange form, from PDB 3MG142. A keto-carotenoid spans the interior of the N- and C-terminal domains (NTD and CTD, respectively), which are connected with a flexible linker and separate upon photoactivation. b Schematic of OCPmore » activation. Upon absorption of blue-green light, OCPO is converted to OCPR, which binds to and quenches an unknown location on the core of the phycobilisome (PB). c Structure of the truncated PB mutant used in this study (CB-PB from Synechocystis PCC 680351). Disc-shaped trimers of phycobiliproteins are joined by linker proteins into stacks that make up the core and rods of the PB. d Bulk fluorescence emission spectrum of PB in the absence (navy) and presence (maroon) of bound OCPR. Solid lines: area-normalized for total relative fluorescence intensity. Dotted line: peak-normalized. e Bulk lifetime decays for PB in the absence (light blue) and presence (light red) of bound OCPR. Single- and double-exponential fits are shown in navy and maroon, respectively. Gray: instrument response function, FWHM 50 ps« less
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Works referenced in this record:

Prokaryotes: The unseen majority
journal, June 1998

  • Whitman, W. B.; Coleman, D. C.; Wiebe, W. J.
  • Proceedings of the National Academy of Sciences, Vol. 95, Issue 12
  • DOI: 10.1073/pnas.95.12.6578

Biomimetic light-harvesting funnels for re-directioning of diffuse light
journal, February 2018

Method for trapping and manipulating nanoscale objects in solution
journal, February 2005

  • Cohen, Adam E.; Moerner, W. E.
  • Applied Physics Letters, Vol. 86, Issue 9
  • DOI: 10.1063/1.1872220

Single-molecule spectroscopy reveals photosynthetic LH2 complexes switch between emissive states
journal, June 2013

  • Schlau-Cohen, G. S.; Wang, Q.; Southall, J.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 27
  • DOI: 10.1073/pnas.1310222110

NMR resonance assignment and backbone dynamics of a C-terminal domain homolog of orange carotenoid protein
journal, September 2020

  • Maksimov, Eugene G.; Laptev, Gennady Yu.; Blokhin, Dmitriy S.
  • Biomolecular NMR Assignments, Vol. 15, Issue 1
  • DOI: 10.1007/s12104-020-09976-1

Electronic coupling of the phycobilisome with the orange carotenoid protein and fluorescence quenching
journal, May 2015

  • Stadnichuk, Igor N.; Krasilnikov, Pavel M.; Zlenko, Dmitry V.
  • Photosynthesis Research, Vol. 124, Issue 3
  • DOI: 10.1007/s11120-015-0148-3

Carotenoid–protein interaction alters the S1 energy of hydroxyechinenone in the Orange Carotenoid Protein
journal, March 2013

  • Polívka, Tomáš; Chábera, Pavel; Kerfeld, Cheryl A.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1827, Issue 3
  • DOI: 10.1016/j.bbabio.2012.10.005

Dissecting pigment architecture of individual photosynthetic antenna complexes in solution
journal, October 2015

  • Wang, Quan; Moerner, W. E.
  • Proceedings of the National Academy of Sciences, Vol. 112, Issue 45
  • DOI: 10.1073/pnas.1514027112

Phycobilisome rod mutants in Synechocystis sp. strain PCC6803
journal, December 2004

Single-molecule diffusometry reveals the nucleotide-dependent oligomerization pathways of Nicotiana tabacum Rubisco activase
journal, March 2018

  • Wang, Quan; Serban, Andrew J.; Wachter, Rebekka M.
  • The Journal of Chemical Physics, Vol. 148, Issue 12
  • DOI: 10.1063/1.5005930

Mass spectrometry footprinting reveals the structural rearrangements of cyanobacterial orange carotenoid protein upon light activation
journal, December 2014

  • Liu, Haijun; Zhang, Hao; King, Jeremy D.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1837, Issue 12
  • DOI: 10.1016/j.bbabio.2014.09.004

Single-Molecule Identification of Quenched and Unquenched States of LHCII
journal, February 2015

  • Schlau-Cohen, Gabriela S.; Yang, Hsiang-Yu; Krüger, Tjaart P. J.
  • The Journal of Physical Chemistry Letters, Vol. 6, Issue 5
  • DOI: 10.1021/acs.jpclett.5b00034

Spectroscopic Properties of the Carotenoid 3‘-Hydroxyechinenone in the Orange Carotenoid Protein from the Cyanobacterium Arthrospira maxima †
journal, March 2005

  • Polívka, Tomáš; Kerfeld, Cheryl A.; Pascher, Torbjörn
  • Biochemistry, Vol. 44, Issue 10
  • DOI: 10.1021/bi047473t

A Soluble Carotenoid Protein Involved in Phycobilisome-Related Energy Dissipation in Cyanobacteria
journal, March 2006

  • Wilson, Adjélé; Ajlani, Ghada; Verbavatz, Jean-Marc
  • The Plant Cell, Vol. 18, Issue 4
  • DOI: 10.1105/tpc.105.040121

A functional compartmental model of the Synechocystis PCC 6803 phycobilisome
journal, July 2017

  • van Stokkum, Ivo H. M.; Gwizdala, Michal; Tian, Lijin
  • Photosynthesis Research, Vol. 135, Issue 1-3
  • DOI: 10.1007/s11120-017-0424-5

Allophycocyanin B (?max 671, 618 nm): A new cyanobacterial phycobiliprotein
journal, January 1975

  • Glazer, A. N.; Bryant, D. A.
  • Archives of Microbiology, Vol. 104, Issue 1
  • DOI: 10.1007/BF00447294

Specificity of the Cyanobacterial Orange Carotenoid Protein: Influences of Orange Carotenoid Protein and Phycobilisome Structures
journal, December 2013

  • Jallet, Denis; Thurotte, Adrien; Leverenz, Ryan L.
  • Plant Physiology, Vol. 164, Issue 2
  • DOI: 10.1104/pp.113.229997

Detection and characterization of single molecules in aqueous solution
journal, November 1996

  • Zander, C.; Sauer, M.; Drexhage, K. H.
  • Applied Physics B Laser and Optics, Vol. 63, Issue 5
  • DOI: 10.1007/BF01828950

Watching Conformational and Photo-Dynamics of Single Fluorescent Proteins in Solution
journal, January 2010

ApcD, ApcF and ApcE are not required for the Orange Carotenoid Protein related phycobilisome fluorescence quenching in the cyanobacterium Synechocystis PCC 6803
journal, August 2012

  • Jallet, Denis; Gwizdala, Michal; Kirilovsky, Diana
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1817, Issue 8
  • DOI: 10.1016/j.bbabio.2011.11.020

Orange carotenoid protein burrows into the phycobilisome to provide photoprotection
journal, March 2016

  • Harris, Dvir; Tal, Ofir; Jallet, Denis
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 12
  • DOI: 10.1073/pnas.1523680113

Regulation of Light Harvesting in Green Plants
journal, June 1996

  • Horton, P.; Ruban, A. V.; Walters, R. G.
  • Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 47, Issue 1
  • DOI: 10.1146/annurev.arplant.47.1.655

Site, Rate, and Mechanism of Photoprotective Quenching in Cyanobacteria
journal, November 2011

  • Tian, Lijin; van Stokkum, Ivo H. M.; Koehorst, Rob B. M.
  • Journal of the American Chemical Society, Vol. 133, Issue 45
  • DOI: 10.1021/ja206414m

Carotenoid-induced quenching of the phycobilisome fluorescence in photosystem II-deficient mutant of Synechocystis sp.
journal, August 2004

Watching conformational- and photodynamics of single fluorescent proteins in solution
journal, January 2010

  • Goldsmith, Randall H.; Moerner, W. E.
  • Nature Chemistry, Vol. 2, Issue 3
  • DOI: 10.1038/nchem.545

Phycobilisomes: Structure and Dynamics
journal, October 1982

Dynamic aspects of phycobilisome structure: Phycobilisome turnover during nitrogen starvation in Synechococcus sp.
journal, January 1980

  • Yamanaka, Gregory; Glazer, Alexander N.
  • Archives of Microbiology, Vol. 124, Issue 1
  • DOI: 10.1007/BF00407026

Dramatic Domain Rearrangements of the Cyanobacterial Orange Carotenoid Protein upon Photoactivation
journal, February 2016

Natural strategies for photosynthetic light harvesting
journal, June 2014

  • Croce, Roberta; van Amerongen, Herbert
  • Nature Chemical Biology, Vol. 10, Issue 7
  • DOI: 10.1038/nchembio.1555

Extensive remodeling of a cyanobacterial photosynthetic apparatus in far-red light
journal, August 2014

A 12 A carotenoid translocation in a photoswitch associated with cyanobacterial photoprotection
journal, June 2015

The Photophysics of the Orange Carotenoid Protein, a Light-Powered Molecular Switch
journal, February 2012

  • Berera, Rudi; van Stokkum, Ivo H. M.; Gwizdala, Michal
  • The Journal of Physical Chemistry B, Vol. 116, Issue 8
  • DOI: 10.1021/jp2108329

Site, Rate, and Mechanism of Photoprotective Quenching in Cyanobacteria
journal, November 2011

  • Tian, Lijin; van Stokkum, Ivo H. M.; Koehorst, Rob B. M.
  • Journal of the American Chemical Society, Vol. 133, Issue 45
  • DOI: 10.1021/ja206414m

Regulation of Light Harvesting in Green Plants
journal, June 1996

  • Horton, P.; Ruban, A. V.; Walters, R. G.
  • Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 47, Issue 1
  • DOI: 10.1146/annurev.arplant.47.1.655

Non-Photochemical Quenching. A Response to Excess Light Energy
journal, April 2001

  • Müller, Patricia; Li, Xiao-Ping; Niyogi, Krishna K.
  • Plant Physiology, Vol. 125, Issue 4
  • DOI: 10.1104/pp.125.4.1558

Orange carotenoid protein burrows into the phycobilisome to provide photoprotection
journal, March 2016

  • Harris, Dvir; Tal, Ofir; Jallet, Denis
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 12
  • DOI: 10.1073/pnas.1523680113

Structure, function and evolution of the cyanobacterial orange carotenoid protein and its homologs
journal, July 2017

  • Kerfeld, Cheryl A.; Melnicki, Matthew R.; Sutter, Markus
  • New Phytologist, Vol. 215, Issue 3
  • DOI: 10.1111/nph.14670

Specificity of the Cyanobacterial Orange Carotenoid Protein: Influences of Orange Carotenoid Protein and Phycobilisome Structures
journal, December 2013

  • Jallet, Denis; Thurotte, Adrien; Leverenz, Ryan L.
  • Plant Physiology, Vol. 164, Issue 2
  • DOI: 10.1104/pp.113.229997

The orange carotenoid protein of Synechocystis PCC 68031Publication No. 15377.1
journal, November 1997

A functional compartmental model of the Synechocystis PCC 6803 phycobilisome
journal, July 2017

  • van Stokkum, Ivo H. M.; Gwizdala, Michal; Tian, Lijin
  • Photosynthesis Research, Vol. 135, Issue 1-3
  • DOI: 10.1007/s11120-017-0424-5

Controlling Light Harvesting with Light
journal, September 2016

  • Gwizdala, Michal; Berera, Rudi; Kirilovsky, Diana
  • Journal of the American Chemical Society, Vol. 138, Issue 36
  • DOI: 10.1021/jacs.6b04811

Phycobilisomes: Structure and Dynamics
journal, October 1982

Mass spectrometry footprinting reveals the structural rearrangements of cyanobacterial orange carotenoid protein upon light activation
journal, December 2014

  • Liu, Haijun; Zhang, Hao; King, Jeremy D.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1837, Issue 12
  • DOI: 10.1016/j.bbabio.2014.09.004

Essential role of two tyrosines and two tryptophans on the photoprotection activity of the Orange Carotenoid Protein
journal, March 2011

  • Wilson, Adjélé; Punginelli, Claire; Couturier, Mohea
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1807, Issue 3
  • DOI: 10.1016/j.bbabio.2010.12.009

Spectroscopic Properties of the Carotenoid 3‘-Hydroxyechinenone in the Orange Carotenoid Protein from the Cyanobacterium Arthrospira maxima †
journal, March 2005

  • Polívka, Tomáš; Kerfeld, Cheryl A.; Pascher, Torbjörn
  • Biochemistry, Vol. 44, Issue 10
  • DOI: 10.1021/bi047473t

Singlet oxygen production in photosystem II and related protection mechanism
journal, September 2008

  • Krieger-Liszkay, Anja; Fufezan, Christian; Trebst, Achim
  • Photosynthesis Research, Vol. 98, Issue 1-3
  • DOI: 10.1007/s11120-008-9349-3

Role of inter-domain cavity in the attachment of the orange carotenoid protein to the phycobilisome core and to the fluorescence recovery protein
journal, July 2015

  • Zlenko, Dmitry V.; Krasilnikov, Pavel M.; Stadnichuk, Igor N.
  • Journal of Biomolecular Structure and Dynamics, Vol. 34, Issue 3
  • DOI: 10.1080/07391102.2015.1042913

Direct single-molecule measurements of phycocyanobilin photophysics in monomeric C-phycocyanin
journal, August 2017

  • Squires, Allison H.; Moerner, W. E.
  • Proceedings of the National Academy of Sciences, Vol. 114, Issue 37
  • DOI: 10.1073/pnas.1705435114

Molecular Mechanism of Photoactivation and Structural Location of the Cyanobacterial Orange Carotenoid Protein
journal, December 2013

  • Zhang, Hao; Liu, Haijun; Niedzwiedzki, Dariusz M.
  • Biochemistry, Vol. 53, Issue 1
  • DOI: 10.1021/bi401539w

Photosynthesis of picoplankton in the oligotrophic ocean
journal, February 1983

  • Platt, Trevor; Rao, D. V. Subba; Irwin, Brian
  • Nature, Vol. 301, Issue 5902
  • DOI: 10.1038/301702a0

Probing Single Biomolecules in Solution Using the Anti-Brownian Electrokinetic (ABEL) Trap
journal, March 2012

  • Wang, Quan; Goldsmith, Randall H.; Jiang, Yan
  • Accounts of Chemical Research, Vol. 45, Issue 11
  • DOI: 10.1021/ar200304t

Carotenoid–protein interaction alters the S1 energy of hydroxyechinenone in the Orange Carotenoid Protein
journal, March 2013

  • Polívka, Tomáš; Chábera, Pavel; Kerfeld, Cheryl A.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1827, Issue 3
  • DOI: 10.1016/j.bbabio.2012.10.005

Prokaryotes: The unseen majority
journal, June 1998

  • Whitman, W. B.; Coleman, D. C.; Wiebe, W. J.
  • Proceedings of the National Academy of Sciences, Vol. 95, Issue 12
  • DOI: 10.1073/pnas.95.12.6578

Excited State Properties of 3′-Hydroxyechinenone in Solvents and in the Orange Carotenoid Protein from Synechocystis sp. PCC 6803
journal, May 2014

  • Niedzwiedzki, Dariusz M.; Liu, Haijun; Blankenship, Robert E.
  • The Journal of Physical Chemistry B, Vol. 118, Issue 23
  • DOI: 10.1021/jp5041794

Single-molecule spectroscopy reveals photosynthetic LH2 complexes switch between emissive states
journal, June 2013

  • Schlau-Cohen, G. S.; Wang, Q.; Southall, J.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 27
  • DOI: 10.1073/pnas.1310222110

Carotenoid-induced quenching of the phycobilisome fluorescence in photosystem II-deficient mutant of Synechocystis sp.
journal, August 2004

A carotenoid-protein from cyanobacteria
journal, October 1981

  • Kay Holt, Thomas; Krogmann, David W.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 637, Issue 3
  • DOI: 10.1016/0005-2728(81)90045-1

Phycobilisome core mutants of Synechocystis PCC 6803
journal, September 1995

  • Ajlani, Ghada; Vernotte, Claudie; DiMagno, Lisa
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1231, Issue 2
  • DOI: 10.1016/0005-2728(95)00086-X

Phycobilisome rod mutants in Synechocystis sp. strain PCC6803
journal, December 2004

Essential role of two tyrosines and two tryptophans on the photoprotection activity of the Orange Carotenoid Protein
journal, March 2011

  • Wilson, Adjélé; Punginelli, Claire; Couturier, Mohea
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1807, Issue 3
  • DOI: 10.1016/j.bbabio.2010.12.009

A comparative study of three signaling forms of the orange carotenoid protein
journal, May 2016

  • Maksimov, E. G.; Moldenhauer, M.; Shirshin, E. A.
  • Photosynthesis Research, Vol. 130, Issue 1-3
  • DOI: 10.1007/s11120-016-0272-8

Structural and functional dynamics of photosynthetic antenna complexes
journal, November 2015

  • Blankenship, Robert E.
  • Proceedings of the National Academy of Sciences, Vol. 112, Issue 45
  • DOI: 10.1073/pnas.1519063112

Local and global structural drivers for the photoactivation of the orange carotenoid protein
journal, September 2015

  • Gupta, Sayan; Guttman, Miklos; Leverenz, Ryan L.
  • Proceedings of the National Academy of Sciences, Vol. 112, Issue 41
  • DOI: 10.1073/pnas.1512240112

Switching an Individual Phycobilisome Off and On
journal, April 2018

  • Gwizdala, Michal; Botha, Joshua L.; Wilson, Adjélé
  • The Journal of Physical Chemistry Letters, Vol. 9, Issue 9
  • DOI: 10.1021/acs.jpclett.8b00767

Single-Molecule Identification of Quenched and Unquenched States of LHCII
journal, February 2015

  • Schlau-Cohen, Gabriela S.; Yang, Hsiang-Yu; Krüger, Tjaart P. J.
  • The Journal of Physical Chemistry Letters, Vol. 6, Issue 5
  • DOI: 10.1021/acs.jpclett.5b00034

Probing Single Biomolecules in Solution Using the Anti-Brownian Electrokinetic (ABEL) Trap
journal, March 2012

  • Wang, Quan; Goldsmith, Randall H.; Jiang, Yan
  • Accounts of Chemical Research, Vol. 45, Issue 11
  • DOI: 10.1021/ar200304t

Direct single-molecule measurements of phycocyanobilin photophysics in monomeric C-phycocyanin
journal, August 2017

  • Squires, Allison H.; Moerner, W. E.
  • Proceedings of the National Academy of Sciences, Vol. 114, Issue 37
  • DOI: 10.1073/pnas.1705435114

Non-Photochemical Quenching and Energy Dissipation in Plants, Algae and Cyanobacteria
book, January 2014

  • Demmig-Adams, Barbara; Garab, Gyozo; Adams III, William
  • Advances in Photosynthesis and Respiration
  • DOI: 10.1007/978-94-017-9032-1

Detection and characterization of single molecules in aqueous solution
journal, October 1996

  • Zander, C.; Sauer, M.; Drexhage, K. H.
  • Applied Physics B: Lasers and Optics, Vol. 63, Issue 5
  • DOI: 10.1007/s003400050118

Controlling Light Harvesting with Light
journal, September 2016

  • Gwizdala, Michal; Berera, Rudi; Kirilovsky, Diana
  • Journal of the American Chemical Society, Vol. 138, Issue 36
  • DOI: 10.1021/jacs.6b04811

Single-Molecule Identification of Coumarin-120 by Time-Resolved Fluorescence Detection:  Comparison of One- and Two-Photon Excitation in Solution
journal, June 1997

  • Brand, L.; Eggeling, C.; Zander, C.
  • The Journal of Physical Chemistry A, Vol. 101, Issue 24
  • DOI: 10.1021/jp963729w

Raman Optical Activity Reveals Carotenoid Photoactivation Events in the Orange Carotenoid Protein in Solution
journal, July 2017

  • Fujisawa, Tomotsumi; Leverenz, Ryan L.; Nagamine, Momoka
  • Journal of the American Chemical Society, Vol. 139, Issue 30
  • DOI: 10.1021/jacs.7b05193

In Vitro Reconstitution of the Cyanobacterial Photoprotective Mechanism Mediated by the Orange Carotenoid Protein in Synechocystis PCC 6803
journal, July 2011

  • Gwizdala, Michal; Wilson, Adjélé; Kirilovsky, Diana
  • The Plant Cell, Vol. 23, Issue 7
  • DOI: 10.1105/tpc.111.086884

The Crystal Structure of a Cyanobacterial Water-Soluble Carotenoid Binding Protein
journal, January 2003

Electronic coupling of the phycobilisome with the orange carotenoid protein and fluorescence quenching
journal, May 2015

  • Stadnichuk, Igor N.; Krasilnikov, Pavel M.; Zlenko, Dmitry V.
  • Photosynthesis Research, Vol. 124, Issue 3
  • DOI: 10.1007/s11120-015-0148-3

Orange Carotenoid Protein as a Control Element in an Antenna System Based on a DNA Nanostructure
journal, January 2017

Single-molecule diffusometry reveals the nucleotide-dependent oligomerization pathways of Nicotiana tabacum Rubisco activase
journal, March 2018

  • Wang, Quan; Serban, Andrew J.; Wachter, Rebekka M.
  • The Journal of Chemical Physics, Vol. 148, Issue 12
  • DOI: 10.1063/1.5005930

Cyanobacterial photoprotection by the orange carotenoid protein
journal, December 2016

Dramatic Domain Rearrangements of the Cyanobacterial Orange Carotenoid Protein upon Photoactivation
journal, February 2016

The time course of non-photochemical quenching in phycobilisomes of Synechocystis sp. PCC6803 as revealed by picosecond time-resolved fluorimetry
journal, September 2014

  • Maksimov, E. G.; Schmitt, F. -J.; Shirshin, E. A.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1837, Issue 9
  • DOI: 10.1016/j.bbabio.2014.01.010

Phycobilisomes Supply Excitations to Both Photosystems in a Megacomplex in Cyanobacteria
journal, November 2013

Phycobilisomes Supply Excitations to Both Photosystems in a Megacomplex in Cyanobacteria
journal, November 2013

A photoactive carotenoid protein acting as light intensity sensor
journal, August 2008

  • Wilson, A.; Punginelli, C.; Gall, A.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 33
  • DOI: 10.1073/pnas.0804636105

The Photophysics of the Orange Carotenoid Protein, a Light-Powered Molecular Switch
journal, February 2012

  • Berera, Rudi; van Stokkum, Ivo H. M.; Gwizdala, Michal
  • The Journal of Physical Chemistry B, Vol. 116, Issue 8
  • DOI: 10.1021/jp2108329

Light Harvesting and Blue-Green Light Induced Non-Photochemical Quenching in Two Different C-Phycocyanin Mutants of Synechocystis PCC 6803
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  • Tian, Lijin; van Stokkum, Ivo H. M.; Koehorst, Rob B. M.
  • The Journal of Physical Chemistry B, Vol. 117, Issue 38
  • DOI: 10.1021/jp309570u

Light Harvesting and Blue-Green Light Induced Non-Photochemical Quenching in Two Different C-Phycocyanin Mutants of Synechocystis PCC 6803
journal, December 2012

  • Tian, Lijin; van Stokkum, Ivo H. M.; Koehorst, Rob B. M.
  • The Journal of Physical Chemistry B, Vol. 117, Issue 38
  • DOI: 10.1021/jp309570u

Molecular Mechanism of Photoactivation and Structural Location of the Cyanobacterial Orange Carotenoid Protein
journal, December 2013

  • Zhang, Hao; Liu, Haijun; Niedzwiedzki, Dariusz M.
  • Biochemistry, Vol. 53, Issue 1
  • DOI: 10.1021/bi401539w

Site of non-photochemical quenching of the phycobilisome by orange carotenoid protein in the cyanobacterium Synechocystis sp. PCC 6803
journal, August 2012

  • Stadnichuk, Igor N.; Yanyushin, Mikhail F.; Maksimov, Evgeni G.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1817, Issue 8
  • DOI: 10.1016/j.bbabio.2012.03.023

Investigation of OCP-triggered dissipation of excitation energy in PSI/PSII-less Synechocystis sp. PCC 6803 mutant using non-linear laser fluorimetry
journal, July 2012

  • Kuzminov, F. I.; Karapetyan, N. V.; Rakhimberdieva, M. G.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1817, Issue 7
  • DOI: 10.1016/j.bbabio.2012.03.022

Watching Conformational and Photo-Dynamics of Single Fluorescent Proteins in Solution
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Singlet oxygen production in photosystem II and related protection mechanism
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  • Krieger-Liszkay, Anja; Fufezan, Christian; Trebst, Achim
  • Photosynthesis Research, Vol. 98, Issue 1-3
  • DOI: 10.1007/s11120-008-9349-3

Photosystem II fluorescence quenching in the cyanobacterium Synechocystis PCC 6803: involvement of two different mechanisms
journal, April 2000

  • El Bissati, K.; Delphin, E.; Murata, N.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1457, Issue 3
  • DOI: 10.1016/s0005-2728(00)00104-3

A photoactive carotenoid protein acting as light intensity sensor
journal, August 2008

  • Wilson, A.; Punginelli, C.; Gall, A.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 33
  • DOI: 10.1073/pnas.0804636105

Role of inter-domain cavity in the attachment of the orange carotenoid protein to the phycobilisome core and to the fluorescence recovery protein
journal, July 2015

  • Zlenko, Dmitry V.; Krasilnikov, Pavel M.; Stadnichuk, Igor N.
  • Journal of Biomolecular Structure and Dynamics, Vol. 34, Issue 3
  • DOI: 10.1080/07391102.2015.1042913

A Soluble Carotenoid Protein Involved in Phycobilisome-Related Energy Dissipation in Cyanobacteria
journal, March 2006

  • Wilson, Adjélé; Ajlani, Ghada; Verbavatz, Jean-Marc
  • The Plant Cell, Vol. 18, Issue 4
  • DOI: 10.1105/tpc.105.040121

Local and global structural drivers for the photoactivation of the orange carotenoid protein
journal, September 2015

  • Gupta, Sayan; Guttman, Miklos; Leverenz, Ryan L.
  • Proceedings of the National Academy of Sciences, Vol. 112, Issue 41
  • DOI: 10.1073/pnas.1512240112

Structural Determinants Underlying Photoprotection in the Photoactive Orange Carotenoid Protein of Cyanobacteria
journal, April 2010

  • Wilson, Adjele; Kinney, James N.; Zwart, Petrus H.
  • Journal of Biological Chemistry, Vol. 285, Issue 24
  • DOI: 10.1074/jbc.M110.115709

The time course of non-photochemical quenching in phycobilisomes of Synechocystis sp. PCC6803 as revealed by picosecond time-resolved fluorimetry
journal, September 2014

  • Maksimov, E. G.; Schmitt, F. -J.; Shirshin, E. A.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1837, Issue 9
  • DOI: 10.1016/j.bbabio.2014.01.010

Orange Carotenoid Protein as a Control Element in an Antenna System Based on a DNA Nanostructure
journal, January 2017

Extensive remodeling of a cyanobacterial photosynthetic apparatus in far-red light
journal, August 2014

A comparative study of three signaling forms of the orange carotenoid protein
journal, May 2016

  • Maksimov, E. G.; Moldenhauer, M.; Shirshin, E. A.
  • Photosynthesis Research, Vol. 130, Issue 1-3
  • DOI: 10.1007/s11120-016-0272-8

Non-Photochemical Quenching. A Response to Excess Light Energy
journal, April 2001

  • Müller, Patricia; Li, Xiao-Ping; Niyogi, Krishna K.
  • Plant Physiology, Vol. 125, Issue 4
  • DOI: 10.1104/pp.125.4.1558

Method for trapping and manipulating nanoscale objects in solution
journal, February 2005

  • Cohen, Adam E.; Moerner, W. E.
  • Applied Physics Letters, Vol. 86, Issue 9
  • DOI: 10.1063/1.1872220

X-ray phase-contrast tomography with a compact laser-driven synchrotron source
journal, April 2015

  • Eggl, Elena; Schleede, Simone; Bech, Martin
  • Proceedings of the National Academy of Sciences, Vol. 112, Issue 18
  • DOI: 10.1073/pnas.1500938112

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    Structural peculiarities of keto-carotenoids in water-soluble proteins revealed by simulation of linear absorption
    journal, January 2019

    • Pishchalnikov, Roman Y.; Yaroshevich, Igor A.; Slastnikova, Tatiana A.
    • Physical Chemistry Chemical Physics, Vol. 21, Issue 46
    • DOI: 10.1039/c9cp04508b
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