Label-free DNA biosensing by topological light confinement
Abstract
Large-area and transparent all-dielectric metasurfaces sustaining photonic bound states in the continuum (BICs) provide a set of fundamental advantages for ultrasensitive biosensing. BICs bridge the gap of large effective mode volume with large experimental quality factor. Relying on the transduction mechanism of reactive sensing principle, herein, we first numerically study the potential of subwavelength confinement driven by topological decoupling from free space radiation for BIC-based biosensing. Then, we experimentally combine this capability with minimal and low-cost optical setup, applying the devised quasi-BIC resonator for PNA/DNA selective biosensing with real-time monitoring of the binding event. A sensitivity of 20 molecules per micron squared is achieved, i.e. ≃0.01 pg. Further enhancement can easily be envisaged, pointing out the possibility of single-molecule regime. This work aims at a precise and ultrasensitive approach for developing low-cost point-of-care tools suitable for routine disease prescreening analyses in laboratory, also adaptable to industrial production control.
- Authors:
-
[1];
[1]
- National Research Council (CNR), Naples (Italy). Institute of Applied Sciences and Intelligent Systems
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Foundry
- National Research Council (CNR), Bari (Italy). Institute of Crystallography
- Univ. of Naples Federico II (Italy)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Materials Sciences & Engineering Division
- OSTI Identifier:
- 1861396
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nanophotonics (Online)
- Additional Journal Information:
- Journal Name: Nanophotonics (Online); Journal Volume: 10; Journal Issue: 17; Journal ID: ISSN 2192-8614
- Publisher:
- de Gruyter
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biosensors; bound states in the continuum; DNA; photonic crystals
Citation Formats
Zito, Gianluigi, Sanità, Gennaro, Guilcapi Alulema, Bryan, Lara Yépez, Sofía N., Lanzio, Vittorino, Riminucci, Fabrizio, Cabrini, Stefano, Moccia, Maria, Avitabile, Concetta, Lamberti, Annalisa, Mocella, Vito, Rendina, Ivo, and Romano, Silvia. Label-free DNA biosensing by topological light confinement. United States: N. p., 2021.
Web. doi:10.1515/nanoph-2021-0396.
Zito, Gianluigi, Sanità, Gennaro, Guilcapi Alulema, Bryan, Lara Yépez, Sofía N., Lanzio, Vittorino, Riminucci, Fabrizio, Cabrini, Stefano, Moccia, Maria, Avitabile, Concetta, Lamberti, Annalisa, Mocella, Vito, Rendina, Ivo, & Romano, Silvia. Label-free DNA biosensing by topological light confinement. United States. https://doi.org/10.1515/nanoph-2021-0396
Zito, Gianluigi, Sanità, Gennaro, Guilcapi Alulema, Bryan, Lara Yépez, Sofía N., Lanzio, Vittorino, Riminucci, Fabrizio, Cabrini, Stefano, Moccia, Maria, Avitabile, Concetta, Lamberti, Annalisa, Mocella, Vito, Rendina, Ivo, and Romano, Silvia. Wed .
"Label-free DNA biosensing by topological light confinement". United States. https://doi.org/10.1515/nanoph-2021-0396. https://www.osti.gov/servlets/purl/1861396.
@article{osti_1861396,
title = {Label-free DNA biosensing by topological light confinement},
author = {Zito, Gianluigi and Sanità, Gennaro and Guilcapi Alulema, Bryan and Lara Yépez, Sofía N. and Lanzio, Vittorino and Riminucci, Fabrizio and Cabrini, Stefano and Moccia, Maria and Avitabile, Concetta and Lamberti, Annalisa and Mocella, Vito and Rendina, Ivo and Romano, Silvia},
abstractNote = {Large-area and transparent all-dielectric metasurfaces sustaining photonic bound states in the continuum (BICs) provide a set of fundamental advantages for ultrasensitive biosensing. BICs bridge the gap of large effective mode volume with large experimental quality factor. Relying on the transduction mechanism of reactive sensing principle, herein, we first numerically study the potential of subwavelength confinement driven by topological decoupling from free space radiation for BIC-based biosensing. Then, we experimentally combine this capability with minimal and low-cost optical setup, applying the devised quasi-BIC resonator for PNA/DNA selective biosensing with real-time monitoring of the binding event. A sensitivity of 20 molecules per micron squared is achieved, i.e. ≃0.01 pg. Further enhancement can easily be envisaged, pointing out the possibility of single-molecule regime. This work aims at a precise and ultrasensitive approach for developing low-cost point-of-care tools suitable for routine disease prescreening analyses in laboratory, also adaptable to industrial production control.},
doi = {10.1515/nanoph-2021-0396},
journal = {Nanophotonics (Online)},
number = 17,
volume = 10,
place = {United States},
year = {Wed Nov 10 00:00:00 EST 2021},
month = {Wed Nov 10 00:00:00 EST 2021}
}
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