Cosolvent enhanced lignocellulosic fractionation tailoring lignin chemistry and enhancing lignin bioconversion

Zhao, Zhi-Min; Meng, Xianzhi; Scheidemantle, Brent; Pu, Yunqiao; Liu, Zhi-Hua; Li, Bing-Zhi; Wyman, Charles E.; Cai, Charles M.; Ragauskas, Arthur J.

doi:10.1016/j.biortech.2021.126367

Title: Cosolvent enhanced lignocellulosic fractionation tailoring lignin chemistry and enhancing lignin bioconversion

Journal Article · Thu Nov 18 00:00:00 UTC 2021 · Bioresource Technology

DOI: https://doi.org/10.1016/j.biortech.2021.126367 · OSTI ID:1843678

Zhao, Zhi-Min ^[1]; Meng, Xianzhi ^[2]; Scheidemantle, Brent ^[3];

^[4]; Liu, Zhi-Hua ^[5]; Li, Bing-Zhi ^[5]; Wyman, Charles E. ^[3]; Cai, Charles M. ^[3];

^[6]

Inner Mongolia Univ., Hohhot (China); Univ. of Tennessee, Knoxville, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States)
Univ. of California, Riverside, CA (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Tianjin Univ., Tianjin (China)
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Cosolvent Enhanced Lignocellulosic Fractionation (CELF) is an emerging solvolysis pretreatment to fractionate lignocellulosic biomass. Herein, the bioconversion performance of CELF lignin was fully evaluated for the first time. Results showed that CELF lignin possessed higher content of carboxylic acid OH, lower molecular weight, and disappeared β-O-4 and β-5 linkages compared to other two technical lignins including a conventional ethanol organosolv lignin (EOL) and a kraft lignin (KL). Rhodococcus opacus PD630 cell count from CELF lignin fermentation reached the highest value of 3.9 10⁷ CFU/mL, representing a 62.5% and 77.3% improvement over EOL and KL, respectively. Correspondingly, lipid yield reached 143 mg/L from CELF lignin, which was 36.2% and 26.5% higher than from EOL and KL, respectively. Principal component analysis (PCA) revealed that more carboxylic acid groups and lower molecular weight contributed to the enhanced bioconversion performance of CELF lignin. This study demonstrates that CELF lignin is a promising candidate for bioconversion.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: National Natural Science Foundation of China; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1843678

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Journal Issue: TBD Vol. 347; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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