Cometabolic Vinyl Chloride Degradation at Acidic pH Catalyzed by Acidophilic Methanotrophs Isolated from Alpine Peat Bogs
Abstract
Remediation of toxic chlorinated ethenes via microbial reductive dechlorination can lead to ethene formation; however, the process stalls in acidic groundwater, leading to the accumulation of carcinogenic vinyl chloride (VC). This study explored the feasibility of cometabolic VC degradation by moderately acidophilic methanotrophs. Furthermore, two novel isolates, Methylomonas sp. strain JS1 and Methylocystis sp. strain MJC1, were obtained from distinct alpine peat bogs located in South Korea. Both isolates cometabolized VC with CH4 as the primary substrate under oxic conditions at pH at or below 5.5. VC cometabolism in axenic cultures occurred in the presence (10 μM) or absence (<0.01 μM) of copper, suggesting that VC removal had little dependence on copper availability, which regulates expression and activity of soluble and particulate methane monooxygenases in methanotrophs. The model neutrophilic methanotroph Methylosinus trichosporium strain OB3b also grew and cometabolized VC at pH 5.0 regardless of copper availability. Bioaugmentation of acidic peat soil slurries with methanotroph isolates demonstrated enhanced VC degradation and VC consumption below the maximum concentration level of 2 μg L–1. Community profiling of the microcosms suggested species-specific differences, indicating that robust bioaugmentation with methanotroph cultures requires further research.
- Authors:
-
[3];
[1]
- Korea Advanced Inst. Science and Technology (KAIST), Daejeon (South Korea)
- National Inst. of Biological Resources (NIBR), Incheon (South Korea)
- Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE; National Research Foundation of Korea (NRF); Ministry of Environment
- OSTI Identifier:
- 1822039
- Grant/Contract Number:
- AC05-00OR22725; 2015M3D3A1A01064881; NIBR201902111
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Environmental Science and Technology
- Additional Journal Information:
- Journal Volume: 55; Journal Issue: 9; Journal ID: ISSN 0013-936X
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; degradation; soils; magnetic properties; genomics; pH
Citation Formats
Choi, Munjeong, Yun, Taeho, Song, Min Joon, Kim, Jisun, Lee, Byoung-Hee, Löffler, Frank E., and Yoon, Sukhwan. Cometabolic Vinyl Chloride Degradation at Acidic pH Catalyzed by Acidophilic Methanotrophs Isolated from Alpine Peat Bogs. United States: N. p., 2021.
Web. doi:10.1021/acs.est.0c08766.
Choi, Munjeong, Yun, Taeho, Song, Min Joon, Kim, Jisun, Lee, Byoung-Hee, Löffler, Frank E., & Yoon, Sukhwan. Cometabolic Vinyl Chloride Degradation at Acidic pH Catalyzed by Acidophilic Methanotrophs Isolated from Alpine Peat Bogs. United States. https://doi.org/10.1021/acs.est.0c08766
Choi, Munjeong, Yun, Taeho, Song, Min Joon, Kim, Jisun, Lee, Byoung-Hee, Löffler, Frank E., and Yoon, Sukhwan. Mon .
"Cometabolic Vinyl Chloride Degradation at Acidic pH Catalyzed by Acidophilic Methanotrophs Isolated from Alpine Peat Bogs". United States. https://doi.org/10.1021/acs.est.0c08766. https://www.osti.gov/servlets/purl/1822039.
@article{osti_1822039,
title = {Cometabolic Vinyl Chloride Degradation at Acidic pH Catalyzed by Acidophilic Methanotrophs Isolated from Alpine Peat Bogs},
author = {Choi, Munjeong and Yun, Taeho and Song, Min Joon and Kim, Jisun and Lee, Byoung-Hee and Löffler, Frank E. and Yoon, Sukhwan},
abstractNote = {Remediation of toxic chlorinated ethenes via microbial reductive dechlorination can lead to ethene formation; however, the process stalls in acidic groundwater, leading to the accumulation of carcinogenic vinyl chloride (VC). This study explored the feasibility of cometabolic VC degradation by moderately acidophilic methanotrophs. Furthermore, two novel isolates, Methylomonas sp. strain JS1 and Methylocystis sp. strain MJC1, were obtained from distinct alpine peat bogs located in South Korea. Both isolates cometabolized VC with CH4 as the primary substrate under oxic conditions at pH at or below 5.5. VC cometabolism in axenic cultures occurred in the presence (10 μM) or absence (<0.01 μM) of copper, suggesting that VC removal had little dependence on copper availability, which regulates expression and activity of soluble and particulate methane monooxygenases in methanotrophs. The model neutrophilic methanotroph Methylosinus trichosporium strain OB3b also grew and cometabolized VC at pH 5.0 regardless of copper availability. Bioaugmentation of acidic peat soil slurries with methanotroph isolates demonstrated enhanced VC degradation and VC consumption below the maximum concentration level of 2 μg L–1. Community profiling of the microcosms suggested species-specific differences, indicating that robust bioaugmentation with methanotroph cultures requires further research.},
doi = {10.1021/acs.est.0c08766},
journal = {Environmental Science and Technology},
number = 9,
volume = 55,
place = {United States},
year = {Mon Apr 12 00:00:00 EDT 2021},
month = {Mon Apr 12 00:00:00 EDT 2021}
}
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